Validation of a national genetic evaluation for methane emission in Holstein cattle.
Lactanet Canada launched a genomic evaluation for methane efficiency for Holsteins in April 2023, utilizing milk mid-infrared-predicted methane (CH4) emissions (CH4MIR) as a proxy. This study validated the methane efficiency genomic evaluation using genotyped cows with CH4MIR and CH4 records from GreenFeed systems (CH4GF), along with relative breeding values (RBV) for methane production and methane efficiency from the April 2023 evaluation. In Lactanet's methane efficiency evaluation, a higher RBV indicates more desirable, lower-emitting animals. For the validation, RBV were categorized into quintiles for the CH4MIR dataset and tertiles for the CH4GF dataset to evaluate trends across the RBV distribution. Mean CH4MIR decreased progressively across RBV quintiles for both traits, with all pairwise comparisons among quintiles significantly different. Similarly, CH4GF emissions declined across RBV tertiles, with significant differences observed between the lowest and highest tertiles. Additional analyses using RBV threshold categories confirmed that cows with the highest RBV consistently exhibited lower methane emissions. Linear regression analyses further demonstrated a negative relationship between RBV and methane emissions, supporting the predictive ability of the genomic evaluation. These findings confirm that Canada's genomic evaluation for methane efficiency effectively differentiates cows by methane emission potential, reinforcing its potential as a tool for genetic selection to reduce methane emissions in dairy cattle.