Biomedical subjects

Chih-Ming Ho

Publications and source records attributed to Chih-Ming Ho.

21 records · Page 2Linked to original sources

PubMed2003

Cloacogenic adenocarcinoma of the vulva presenting as recurrent Bartholin's gland infection.

Primary adenocarcinoma of the vulva of the cloacogenic type is extremely rare. We report the case of a 49-year-old patient with a 6-year history of recurrent left Bartholin's gland infection and a 1-year history of an ulcerated tumor on the similar lower aspect of the left labium majus. Fine needle biopsy revealed adenocarcinoma and wide local excision with bilateral superficial inguinal lymph node dissection was performed. The patient was well and disease-free at 24 months after the operation. This case provides a good example of why an early biopsy should be performed for any persistent anogenital lesion. Although the reported data on cloacogenic adenocarcinoma are limited, a wide local excision appears to be the most widely used treatment strategy for any vulvar cancer of 2 cm or less in diameter.

Adenocarcinoma↗

PubMed2002

An electrochemical detection scheme for identification of single nucleotide polymorphisms using hairpin-forming probes.

Single nucleotide polymorphisms are implicated as having a significant role in regulating growth, development and, thereby, human health and disease. We have developed a method for identifying single nucleotide genetic alterations by combining hairpin-forming DNA probes and electrochemical detection of sandwich DNA hybridization. Incorporation of hairpin-forming competitor probes and the catalyzed reporter deposition amplification system further improves assay specificity by 7-fold and sensitivity by 100-fold. We have demonstrated that the system successfully identified the factor V Leiden mutations from human blood specimens.

DNA Probes↗

PubMed2002

Endothelial cell dynamics under pulsating flows: significance of high versus low shear stress slew rates (d(tau)/dt).

Shear stress modulates endothelial cell (EC) remodeling via realignment and elongation. We provide the first evidence that the upstroke slopes of pulsatile flow, defined as shear stress slew rates (positive d(tau)/dt), affect significantly the rates at which ECs remodel. We designed a novel flow system to isolate various shear stress slew rates by precisely controlling the frequency, amplitude, and time-averaged shear stress (tau(ave)) of pulsatile flow. Bovine aortic endothelial cell (BAEC) monolayers were exposed to three conditions: (1) pulsatile flow (1 Hz) at high slew rate (293 dyn/cm2 s), (2) pulsatile flow (1 Hz) at low slew rate (71 dyn/cm2s), and (3) steady laminar flow at d(tau)/dt = 0. All of the three conditions were operated at tau(ave) = 50 dyn/cm2. BAEC elongation and alignment were measured over 17 h. We were able to demonstrate the effects of shear stress slew rates ((tau)/dt) on EC remodeling at a fixed spatial shear stress gradient (d(tau)/dx). We found that pulsatile flow significantly increased the rates at which EC elongated and realigned, compared to steady flow at d(tau)/dt = 0. Furthermore, EC remodeling was faster in response to high than to low slew rates at a given tau(ave).

Animals↗