Biomedical subjects

Chien-Hung Liu

Publications and source records attributed to Chien-Hung Liu.

3 recordsLinked to original sources

PubMed2006

Characterization of the Soj/Spo0J chromosome segregation proteins and identification of putative parS sequences in Helicobacter pylori.

The Soj and Spo0J proteins, together with one or more parS sequences, are crucial to chromosome segregation and the progression of cell cycle in many bacteria. In Helicobacter pylori, genes coding for Soj and a plasmid replication-partition-related protein containing a Spo0J or ParB conserved domain, together with two putative parS sites identified in this study, were found to be located within the origin-proximal 20-30% of the circular chromosome. Recombinant H. pylori Spo0J bound specifically to the two putative parS sequences and that of Bacillus subtilis. In addition, hydrolysis of ATP by H. pylori Soj was accelerated in the presence of parS and/or Spo0J. Protein-protein interactions, intracellular levels, and subcellular localization of Soj and Spo0J were analyzed through polyclonal antibodies directed against recombinant Soj and Spo0J. This study was the first implication of the existence of a functional parABS system in H. pylori.

Adenosine Triphosphatases↗

PubMed2004

Development of a diffraction-type optical triangulation sensor.

We propose a diffraction-type optical triangulation sensor based on the diffraction theorem and the laser triangulation method. The advantage of the proposed sensor is that it obtains not only the linear displacement of a moving object but also its three angular motion errors. The developed sensor is composed mainly of a laser source, two quadrant detectors, and a reflective diffraction grating. The reflective diffraction grating can reflect the incident laser beam into several diffractive rays, and two quadrant detectors were set up for detecting the position of 0- and + 1-order diffraction rays. According to the optical triangulation relationship between the spatial incident angles of a laser beam and the output coordinates of two quadrant detectors, the displacement and the three angular motion errors of a moving object can be obtained simultaneously.

Journal Article↗

PubMed2004

Simple three-dimensional laser angle sensor for three-dimensional small-angle measurement.

A simple three-dimensional (3D) laser angle sensor for 3D measurement of small angles based on the diffraction theorem and on ray optics analysis is presented. The possibility of using position-sensitive detectors and a reflective diffraction grating to develop a 3D angle sensor was investigated and a prototype 3D laser angle sensor was designed and built. The system is composed of a laser diode, two position-sensitive detectors, and a reflective diffraction grating. The diffraction grating, mounted upon the rotational center of a 3D rotational stage, divides an incident laser beam into several diffracted rays, and two position-sensitive detectors are set up for detecting the positions of +/-1st-order diffracted rays. According to the optical path relationship between the three angular motions and the output coordinates of the two position-sensitive detectors, the 3D angles can be obtained through kinematic analysis. The experimental results show the feasibility of the proposed 3D laser angular sensor. Use of this system as an instrument for high-resolution measurement of small-angle rotation is proposed.

Journal Article↗