Biomedical subjects

Changhe Zhou

Publications and source records attributed to Changhe Zhou.

4 recordsLinked to original sources

PubMed2003

Multifunctional double-layered diffractive optical element.

We propose a novel multifunctional double-layered diffractive optical element (DOE) based on the fractional Talbot effect. This DOE consists of two layers: one is the encoding layer, in which multiple sub-DOEs, i.e., multiple optical functions, are encoded; the other is the decoding layer, which is a properly designed Talbot illuminator. This DOE can perform each of the multiple optical functions one by one by shifting the encoding layer. Experimental results demonstrate that this method is efficient. This device should be highly interesting for integrated optics, optical interconnection, secure optical storage, and dynamic optical fiber communications.

Journal Article↗

PubMed2003

Miniaturized three-dimensional endoscopic imaging system based on active stereovision.

A miniaturized three-dimensional endoscopic imaging system is presented. The system consists of two imaging in channels that can be used to obtain an image from an object of interest and to project as tructured light onto the imaged object to measure the surface topology. The structured light was generated with a collimated monochromatic light source and a holographic binary phase grating. The imaging and projection channels were calibrated by use of a modified pinhole camera. The surface profile was extracted by use of triangulation between the projected feature points and the two channel ofthe endoscope. The imaging system was evaluated in three-dimensional measurements of several objects with known geometries. The results show that surface profiles of the objects with different surfaces and dimensions can be obtained at high accuracy. The in vivo measurements at tissue sites of human skin and an oral cavity demonstrated the potential of the technique for clinical applications.

Depth Perception↗

PubMed2003

Repression of gene expression by Arabidopsis HD2 histone deacetylases.

The four HD2 proteins of Arabidopsis thaliana (AtHD2A-D) belong to a unique class of histone deacetylases that is plant specific. Previously, we have demonstrated that one of the members, AtHD2A, can mediate transcriptional repression when targeted to the promoter of a reporter gene. Here, we report that AtHD2B and AtHD2C can also repress gene expression. AtHD2A and AtHD2C differ from AtHD2B and AtHD2D in the composition of their structural domains. Our data show that both structural types play a role in the repression of gene transcription. We demonstrate that AtHD2A can mediate gene repression through interactions with transcription factors in plants. By fusing AtHD2A with the DNA-binding domain of the plant transcriptional factor Pti4, the expression of a GCC box containing reporter gene was repressed. We also demonstrated repression of a GUS gene with GAL4 enhancers using transgenic plants that expressed a GAL4/AtHD2A fusion gene. Furthermore, the expression of the GAL4/AtHD2A protein using the seed-specific napin promoter (NAP2) and the constitutive tCUP promoter demonstrated that repression of transgenes could be achieved in a tissue-specific or unrestricted manner. Targeting of HD2 proteins to specific promoters using transcription factor DNA-binding domains may therefore provide a new technology for silencing target genes and pathways in plants as well as for assessing the function of unknown transcription factors.

Arabidopsis↗

PubMed2002

Nonvolatile holograms in LiNbO3:Fe:Cu by use of the bleaching effect.

We report our observation of a bleaching effect under an ultraviolet exposure in LiNbO3:Fe:Cu crystals. Two three-step recording-transferring-fixing schemes are proposed to record nonvolatile photorefractive holograms in such crystals. In the schemes two red laser beams and an ultraviolet illumination are used selectively to write the charge grating inthe shallow-level Fe centers, to develop the charge grating inthe deep-level Cu centers by transferring the charge grating in the Fe centers, and to fix only thecharge grating in the Cu centers for unerasable read-out. Experimental results, verifications, and an optimal recording scheme are given. A comparison of the lithium niobate crystals of the same double-doping system of Fe:Mn, Ce:Mn, Ce:Cu, and Fe:Cu is outlined.

Journal Article↗