Biomedical subjects

Casey J LeBlanc

Publications and source records attributed to Casey J LeBlanc.

4 recordsLinked to original sources

PubMed2007

Serial alterations in digital hemodynamics and endothelin-1 immunoreactivity, platelet-neutrophil aggregation, and concentrations of nitric oxide, insulin, and glucose in blood obtained from horses following carbohydrate overload.

OBJECTIVE: To quantify changes in endothelium-derived factors and relate those changes to various aspects of digital hemodynamics during the prodromal stages of carbohydrate overload (CHO)-induced laminitis in horses. ANIMALS: 20 adult horses without abnormalities of the digit. PROCEDURES: Digital and jugular venous blood samples were collected at 1-hour intervals (for assessment of endothelin-1 [ET-1] immunoreactivity and measurement of glucose, insulin, and nitric oxide [NO] concentrations) or 4-hour intervals (CBC and platelet-neutrophil aggregate assessment) for 8 hours or 16 hours after induction of CHO-associated laminitis in horses treated with an ET-1 antagonist. Effects of treatment, collection site, and time and the random effects of horse on each variable were analyzed by use of a repeated-measures model. Where treatment and collection site had no significant effect, data were combined. RESULTS: Compared with baseline values, CHO resulted in changes in several variables, including a significant increase from baseline in digital blood ET-like immunoreactivity at 11 hours; digital blood ET-like immunoreactivity was significantly greater than that in jugular venous blood at 8, 9, 11, and 12 hours. Digital and jugular venous blood concentrations of glucose increased from baseline significantly at 3, 4, and 5 hours; insulin concentration increased significantly at 5 hours; and the number of platelet-neutrophil aggregates increased significantly at 12 hours. CONCLUSIONS AND CLINICAL RELEVANCE: In horses, concurrent increases in venous blood ET-1 immunoreactivity, insulin and glucose concentrations, and platelet-neutrophil aggregates support a role of endothelial dysfunction in the pathogenesis of CHO-induced laminitis.

Animals↗

PubMed2005

Effect of dietary fish oil and vitamin E supplementation on hematologic and serum biochemical analytes and oxidative status in young dogs.

Fifteen healthy dogs received a basal diet supplemented with either 12.4 g of sunflower oil, 0.6 g of sunflower oil and 7 g of menhaden fish oil, or 0.6 g of sunflower oil and 7 g of menhaden fish oil plus 0.18 g of alpha-tocopherol acetate for twelve weeks. There was no significant diet effect on platelet aggregation, lipid peroxidation, or standard hematologic and biochemical parameters, with the exception of decreased triglycerides in dogs supplemented with fish oil. These data demonstrate that this level of fish oil supplementation in dogs does not require vitamin E supplementation above recommended dosage and may prove beneficial in the treatment of hyperlipidemia in the dog.

Animals↗

PubMed2004

Firm rib mass aspirate from a dog.

A 9-year-old intact male Miniature Schnauzer presented laterally recumbent, cachexic, and dehydrated with multiple firm bone masses and a bilaterally enlarged prostate. Fine-needle aspiration of a rib mass revealed numerous basophilic polygonal to fusiform cells predominantly found in small to large clusters. The cells exhibited cytologic criteria of malignancy and infrequently displayed large cytoplasmic vacuoles containing finely- to coarsely-stippled azurophilic material. The cytologic diagnosis was metastatic adenocarcinoma and was suspected to be prostatic or transitional cell in origin because of the azurophilic vacuoles within malignant cells. Gross and histologic findings confirmed the clinical and cytologic diagnosis of prostatic adenocarcinoma with widespread metastasis. Cytochemical and immunohistochemical investigation confirmed glycogen was a component of the vacuolar material. The vacuoles observed in the tumor in this case are not a consistent finding in tumors of the prostate or urinary bladder; however, when associated with an epithelial tumor, they may aid in limiting the differentials of the primary tumor and in the selection of further diagnostics.

Adenocarcinoma↗

PubMed2003

Passive sampling screening method using thin-layer chromatography plates.

An in situ solid-phase extraction method, thin-layer chromatography (TLC) passive sampling, was investigated as a screening method for determining the presence of organic compounds in water using laboratory experiments and field applications. The TLC passive sampler developed in this research enables the spatial and temporal distributions of organic compounds to be determined qualitatively with little expense. The materials for the sampler developed here cost dollar 1.60 each. By first identifying the areas where a pollutant is present using the TLC passive sampling screening method, total analytical costs for monitoring programs may be reduced by eliminating unnecessary conventional analyses for locations where the pollutant is not present. Two organophosphorus pesticides, diazinon and chlorpyrifos, were used as a model for the development and as a potential application of the TLC passive sampling method. Passive sampler adsorption kinetics, enrichment factors, and extraction efficiencies were determined from batch experiments with exposure times ranging from 1 h to 4 weeks. In field applications, TLC passive sampling was conducted in natural and engineered systems with two silica gel extraction media, C2 and C18. Diazinon and chlorpyrifos were detected by analyzing the adsorbed compounds by high-performance liquid chromatography with ultraviolet detection.

Chromatography, High Pressure Liquid↗