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Biomedical subjects

C Zinn

Publications and source records attributed to C Zinn.

At least 37 records · Page 2Linked to original sources
PubMed1997

Characteristics of hypersensitivity reactions and identification of a unique 49 kd IgE-binding protein (Hal-m-1) in abalone (Haliotis midae).

BACKGROUND: There is a paucity of published data on the clinical presentation and the nature of the allergens involved in hypersensitivity to mollusks. This study reports the clinical and immunologic findings in 38 patients with reported immediate and delayed adverse reactions to abalone (Haliotis midae, Class Gastropoda). METHODS: Patients were recruited as part of a South African seafood allergy survey. Allergic symptoms were assessed by a self-administered questionnaire. A total of 38 patients with abalone sensitivity were recruited. Specific IgE responses to abalone and other mollusks were studied by using RAST and inhibition ELISAs. Skin prick tests and lymphocyte proliferation assays were also performed on several of the subjects. Allergenic components of Haliotis midae were identified with Western blotting. RESULTS: Twenty five of the 38 patients in the study were first seen with immediate symptoms, and 13 had delayed reactions. Seventeen of the sera tested were RAST positive. Skin prick tests responses with abalone extract were positive in all subjects with positive RAST responses (n = 8) and in 6 of 13 subjects with negative RAST responses. Five of the subjects with positive RAST responses had positive results on Western blotting and demonstrated binding to two major allergens with molecular weights of 38 and 49 kd. The 49 kd IgE-binding protein has been designated as Hal-m-1. CONCLUSIONS: Abalone allergens are heat-stable proteins with molecular weights of 38 and 49 kd, later designated as Hal-m-1 according to International Union of Immunological Societies allergen nomenclature regulation. Our studies indicate a clear clinical and immunologic heterogeneity in patients reactive to abalone.

Adolescent↗
PubMed1997

The spectrum of allergy to South African bony fish (Teleosti). Evaluation by double-blind, placebo-controlled challenge.

OBJECTIVE: The aim of this study was to assess the spectrum of allergy to South African bony fish (Class Teleosti), crustaceans and molluscs and to confirm or refute suspected allergy, specifically to bony fish, by double-blind, placebo-controlled food challenge (DBPCFC). DESIGN: Patients were recruited by means of a seafood allergy questionnaire. Subjects with reported allergy to hake, yellowtail, salmon and mackerel were investigated by means of skin-prick tests, RASTs and Western blot analysis. For those subjects with test results that were either all negative or equivocal, a definitive diagnosis of clinical sensitivity was made on the basis of DBPCFC. SETTING: Volunteer population-based cohort in the Western Cape. PARTICIPANTS: 105 volunteer subjects with suspected fish allergy were recruited by advertising in the local press. MAIN OUTCOME: Species-specific bony fish allergy was confirmed or refuted by DBPCFC. RESULTS: The four most common seafood species reported to cause adverse reactions were prawns (46.7%), crayfish (43.8%), abalone (35.2%) and black mussels (33.3%). The four most common bony fish species to cause reactions were hake (24.8%), yellowtail (21.9%), salmon (15.2%) and mackerel (15.2%). Seven DBPCFCs were performed and two open challenges. Skin-prick tests produced one false-negative result. Western blots produced one false-negative and one false-positive result. The RAST had a 100% correlation with DBPCFC. CONCLUSIONS: Local bony fish represent a significant cause of clinical reactions to seafood in the Western Cape. Although skin-prick tests, RASTs and Western blotting tests assist in the documentation of an IgE responder state, confirmation of clinical sensitivity can only be made with certainty by means of DBPCFC.

Adolescent↗