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Biomedical subjects

C Zhou

Publications and source records attributed to C Zhou.

At least 145 records · Page 8Linked to original sources
PubMed1998

[Infection of cryptosporidium in child patients with diarhea in Changsha].

Fecal specimens were collected from 102 outpatients with diarrhea in Children's Hospital from March to October, 1997. The fecal smears were examined for cryptosporozoites with the rapid modified acid-fast two-step methods. The positive rate was 4.90%(5/102). There was no significant difference between female and male (P > 0.05). The positive rates of 1-month-, 5-year-, and 10-year-old groups were 4.05% (3/74), 7.14%(1/14), and 7.14(1/14), respectively. We conclude that there is crytosporidium infection in child patients with diarrhea in different sex and age groups.

Adolescent↗
PubMed1998

[The clinical evaluation of endometrial carcinoma following irradiation for cervical cancer].

OBJECTIVE: To study the characteristics and clinical features of endometrial carcinoma occurred after pelvic irradiation. METHODS: Clinical data of 27 cases of endometrial carcinoma occurred after irradiation for cervical cancer were reviewed, estrogen receptor (ER) and progesterone receptor (PR) status were studied in 17 cases by immunohistochemistry. RESULTS: The incidence of endometrial carcinoma occurred following pelvic irradiation was 0.19%. The mean age of patients was 63 year old. The mean incubation period from pelvic irradiation to diagnosis of the disease was 15.6 years. The proportion of advanced cases was 51.9% (14/27). The expression of ER and PR was very low (11.8%, 2/17). The original sites of 5 cases with stage I were in fundus or corner of the uterus, which received lower dose irradiation formerly. Operation was the best choice of treatment, 19 out of 27 cases received this modality. The survival rate of 3 and 5 year was 56.8%, 37.8%, respectively. CONCLUSION: The incidence of endometrial carcinoma occurred following pelvic irradiation for cervical cancer was higher than that of cancer occurred spontaneously, the proportion of advanced cases was high, the expression of ER and PR was very low, operation was the mainly method of treatment. The prognosis was poorer than that of spontaneous disease.

Aged↗
PubMed1998

[Telomerase activity in human primary endometrial carcinoma].

OBJECTIVE: To investigate the possible relationship between telomerase expression and progression of endometrial carcinoma. METHODS: Thirty-four primary endometrial carcinoma and 6 normal endometrial tissue specimens were examined by means of telomeric repeat amplification protocol (TRAP) assay for telomerase activity. RESULTS: Of 34 endometrial cancer specimens, 28 (82.4%) showed telomerase activity, whereas 1 of 6 (16.7%) benign endometrial tissues exhibited telomerase activity. CONCLUSION: Telomerase expression might contribute to the progression of endometrial carcinoma and could be used as its tumor marker.

Adenocarcinoma↗
PubMed1998

[CT findings of calcified liver metastases].

OBJECTIVE: To observe the CT findings of 13 cases with calcified liver metastases. METHODS: The primary tumors were 10 cases of colonic carcinoma and 3 cases of rectal carcinoma. CT and ultrasonic examinations of these 13 cases revealed calcified liver metastases. One case had laparotomy and biopsy, pathological diagnosis was liver metastases with necrosis and focal calcifications. RESULTS: There were 3 types of CT findings of calcified liver metastases: (1) sand-like calcifications conglomerate into a mass or circular clump, (2) irregular patchy calcifications, (3) small punctate calcifications. These calcifications were either in the center or the periphery of a single or multiple low density masses. CONCLUSION: Calcified liver metastases are uncommon. The primary tumor is usually colon carcinoma. Calcification can also be seen in carcinomas of the ovary, stomach and breast metastasized to the liver and primary carcinoma of the liver. When calcified liver metastasis is revealed by CT in patient with unknown primary tumor, colon cancer may be suggested.

Adult↗
PubMed1998

[Ovarian metastasis from endometrial carcinoma: a report of 22 cases].

OBJECTIVE: To study the clinical characteristics of ovarian metastasis from endometrial carcinoma and its relation to pathologic features and prognosis. METHODS: Twenty-two cases of histologically confirmed ovarian metastasis from endometrial carcinoma were retrospectively reviewed. Prognostic factors such as clinical staging, grade, myometrial infiltration, recurrence and treatment methods were analyzed. RESULTS: The median age of this series was 50.6. Incidence of ovarian metastasis accounted for 4.4% in the same period. Ten out of 22 cases were in clinical stage I, 6 in stage II, and 6 in stage III. The treatment of choice was operation combined with radiotherapy. The overal 5- and 10-year survival rate was 61.5 and 55.9%, respectively. CONCLUSION: Ovarian metastasis from endometrial carcinoma is associated with poor prognosis. Aggressive treatment after operation is required. For young patients in early stage of the disease, circumscript attitude should be taken as to preserve the ovary or not.

Adenocarcinoma↗
PubMed1998

[Construction and secretory expression of single-chain Fv fragment M97 with therapeutic potential against tumor invasion and metastasis].

OBJECTIVE: To manufacture single chain Fv antibody fragment for cancer targeting therapy. METHODS: Based on recombinant phage display techniques, the construction, screening and expression of functional single-chain Fv fragment (scFv) from murine hybridoma C2H5 which secretes antibody specifically binding to (92 kD) type IV collagenase were performed. By RT-PCR for VH and VL genes, assembly of scFv genes and cloning into phagemid pCANTAB5E, transformation of TG1 cells and M13KO7 helper phage rescue, a recombinant phage scFv library with titre of 5 x 10(9) pfu/ml was established. Panning against immobilized type IV collagenase (92 kD) was performed by one round. Finally, 30 antigen-positive recombinant phage clones were isolated and identified by ELISA. RESULTS: DNA sequencing result showed that the entire gene of scFv against type IV collagenase (92 kD) designated as scFv-M97 is 732 bp, consisting of the 351 bp VH gene encoding 117 amino acids, the 336 bp VL gene encoding 112 amino acids and a 45 bp linker encoding (Gly4Ser)3. The antigen-positive phage with titre of 10(10) pfu/ml was used to infect HB2151 cells in which soluble scFv was expressed. After induction of Lac promotor by 1 mM IPTG for 20 h, the scFv-M97 yield of HB2151 in SBA medium is about 2 micrograms/ml. Immuno-blot indicated that the 27 kD scFv-M97 retained the affinity and specificity of the original intact antibody to type IV collagenase (92 kD). CONCLUSIONS: Single chain antibody scFv-M97 expressed by secretion was successfuly produced. As type IV collagenase plays an important role in tumor invasion and metastasis by the mechanism of proteolytic degradation of type IV collagen in the basement membrane, scFv-M97 may have therapeutic potential against tumor invasion and metastasis.

Amino Acid Sequence↗
PubMed1998

[Clinical study on Shenqi Fuzheng injection combined with chemotherapy in treating malignant tumor of digestive tract].

OBJECTIVE: To assess the clinical effect and safety of Shenqi Fuzheng Injection (SQFZI) combined with chemotherapy in treating malignant tumor of digestive tract. METHODS: One hundred and eighty-two patients were randomly divided into 3 groups, the combined therapy group (Group A), the SQFZI group (Group B) and the chemotherapy group (Group C). The remission and stabilization effect on cancer were observed. RESULTS: The remission rate and stabilizing rate of Group A were 20.2% and 87.9%, and those of the Group C were 15.6% and 68.9%, the difference between the two groups was significant, P < 0.05. The symptom and living quality improving rate of Group A were 78.8% and 42.4% separately, those of the Group B were 73.7% and 68.4% and of the Group C 37.8% and 26.7% respectively, the inter-group differences were significant, P < 0.05. The weight gain rate and effective rate of Group A were 45.5% and 90.9%, of Group C were 13.3% and 40.0%, P < 0.05. SQFZI showed good protective effect on hematopoietic system, 7.7% patients in Group A with WBC count lower than 4 x 10(9)/L after treatment, while in Group C, the percentage reached 22.2%. Moreover, SQFZI could raise activity of NK cell, macrophage and T-lymphcyte subgroups, without any injury on heart, liver and kidney function or other adverse reaction. CONCLUSION: SQFZI combined with chemotherapy has certain effect of remitting and stablizing on digestive tract tumor, the clinical effect is significant to patients with Qi-Deficiency, and is safe and reliable.

Adult↗
PubMed1998

[A study on EEG-Encephalofluctuographic characteristics in alpha frequency band in pilots].

The frequency-fluctuation characteristics in alpha frequency band were studied by EEG-Encephalofluctuographic Technology (EEG-ET) in 261 pilots. The electrical activity was recorded from 12 scalp monopolar leads of the international 10-20 system using linked ear lobes as reference. The components of the competitive frequency-fluctuation structure in alpha frequency band was analysed. The results showed that the predominant frequency component of the competitive structure was 9 Hz in the 261 normal healthy pilots. The second was 10 Hz. And the third was 7 Hz. The other competitive frequency components were inhibited. The relative entropy value (percentage) of the competivity structure was calculated for each EEG derivation of each pilot. We also analysed the spectra of the EEG-Encephalofluctuograph. We found that there was a dominant power peak at 21.39mHz in 57 pilots who complained in-flight blackout sometimes during acceleration.

Acceleration↗
PubMed1998

[Ergonomical study on Chinese speech warning].

Ergonomical experiments on speech warning under noise background were carried out in 40 healthy males, aged 20-33. Through the determination of auditory reaction time to the Chinese speech warning under dual-tasks and the subjective evalution of the suitable time length of main warning voice by the subject, the optimum parameters of Chinese speech warning in accordance with space ergonomics were determined. It was found that: suitable time length of main warning voice is 0.35-0.55s, main interval is 0.15-0.35s, speech speed is 4-6 word/s, and sentence interval is 0.2-0.4s. Meanwhile, the analysis of heart rate (HR) and heart rate variability (HRV) demonstrated that the speech warning using aforementioned parameters didn't increase the operator's work load. The results can serve as the objective ergonomical basis and the evaluation criterion for design of speech warning in manned space vehicle.

Adult↗
PubMed1998

[Psychological study of parameter selection for Chinese speech warning].

To study the effects of the parameters of Chinese speech warning on cognitive property, and to obtain the optimal parameters in accordance with cognitive property, experiments of cognitive property for Chinese speech warning was carried out in 20 healthy males. Combining our study on the sensation-reaction time, the optimal parameters in accordance with space ergonomics [correction of ergonormics] and cognitive property were determined: suitable time length of prewarning sound is 0.35 approximately 0.55 s; prewarning interval is 0.3-0.4 s; interval between sentences is 0.3-0.4 s; word number of warning sentence < or = 7. The result can serve as the objective ergonomical basis for the design of Chinese speech warning system in manned space vehicle, and as the evaluation criterion.

Adult↗
PubMed1998

[Determination of organochlorine pesticide residues in herba Asari by GC].

OBJECTIVE: To provide a theoretical guide for the production and application of Herba Asari. METHOD: A GC method for multiresidue analysis was used. RESULT: Fifteen samples of Herba Asari were detected with the method for the first time. The contents of organochlorine pesticide residues in most of the samples were under the Chinese national limits for grains and green vegetables (GB 2707-2763-81). The other samples were polluted seriously. CONCLUSION: Some suggestions have been put forward to help keep down pesticide residue pollution in Chinese herbal medicines.

Chromatography, Gas↗
PubMed1998

[Problems and solutions in the use of RAPD to the identification of the Chinese drugs "xi-xin" (Herba asari) and its substitutes].

The use of random amplified polymorphic DNAs (RAPD) on the identification of crude drugs has a few special problems, e.g., the influence of the degree of degradation of DNA templates on the amplified products, the reliability of results by one or two primers and the application range of RAPD, etc., so the problems were discussed through researches on Herba Asari. In this experiment, the DNA of twenty-one samples from different locations and collected at different time were extracted. The suitable concentrations of DNA templates and the suitable primers were selected. After this, the DNA of these samples were amplified by RAPD. The results showed that the concentration and the degree of degradation of DNA templates, and the different sources of crude drugs affected the result of RAPD assay. Some suggestions were made to solve these problems, such as the selection of DNA templates concentration, the screening of suitable primers, and the use of contrast groups and cluster analysis. Furthermore, the application range of RAPD to the identification of crude drugs was discussed. All of these will provide the guide to the use of RAPD for the identification of crude drugs.

Asarum↗
PubMed1998

[Evaluation on the anti-hepatitis C virus enzyme-linked immunosorbent diagnostic kits].

Ten different anti-HCV ELISA kits, four manufactured from abroad (ABBOTT, Innogenetics, Murex, Monolisa) and six in China, were evaluated by China national anti-HCV panel(100 positive, 98 negative) and Boston Biomedical Inc. (BBI) anti-HCV mixted titer performance panel(23 positive and 2 negative). The detective rates in 100 China national anti-HCV positive panel were 98% by ABBOTT EIA 2.0, 96% by BHY, SKH and XWK, 95% by BBS and HHM, 97% by SCJ, and that in 23 BBI anti-HCV positive panel were 100% by ABBOTT EIA2.0, but 91% by Murex, 87% by Innogenetics and Monolisa, 74% by BHY, 87% by SKH, 83% by XWK and HHM, 70% by BBS, 91% by SCJ. The specificity for all negative panels were similar in all kits. According to the results of HCV RIBA test (Ortho HCV RIBA 2.0), the structural antigens contained in all kits were almost the same, but the antigenicity of HCV nonstructural proteins were different, in the kits made in China they were lower than that made from abroad.

Antibodies, Viral↗
PubMed1998

[Study on pharmacological actions of pingxiaozhitong pill].

Pingxiaozhitong pill was administered ig. once daily for 7 successive days at dosages (4,2,2 g/kg). It was shown markelly suppressive effects on solid sarcoma 180 (S180) and Ehrlich aseites carcinoma (EAC) and synergism effect on cytoxan inhibiting S180 in mice. It had significant analgesic effect. 4,2 g/kg pingxiaozhitong pill enhanced effects on phagocysis of monocyte macrophage and value of hemagglutinin in serum in mice.

Adjuvants, Immunologic↗
PubMed1998

[A caries survey in Shanghai Penglai district]

OBJECTIVE: Investigate the oral epidemic disease occurance to get the essential data and provide the standard for supervising the result of education of oral health and the measures of oral epidemic disease probation.METHODS:Random sample by equal proportion and divide into groups.The standard of second whole national investigation of oral epidemic disease is accepted in this investigation.RESULTS:The caries prevalence rate are:5 year old group 63.1% 12 year-old group 28.6% 15 year-old group 31.8%,18 year-old group 43.6%,35-44 year-old group 41.3%,65-74 year-old group 61.8%.CONCLUSION:This statistics states:(1)The rates of caries have distinguish differences between different age groups.(2)The caries prevalence rate and the DMFT is rather less than the result of Shanghai area which join the second whole national investigation of oral epidemic disease.

Journal Article↗
PubMed1998

[Application of three-dimension luminescence method to the identification of petroleum pollutants].

As three-dimension luminescence method can provide abundant sample information, it is applied to the identification of petroleum pollutants. In this paper, the whole analytical process of three-dimension luminescence method is described, including sample disposal, analytical conditions, analytical process, especially the automation in acquisition, disposal and differentiation of analytical data.

English Abstract↗