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Biomedical subjects

C Y Lin

Publications and source records attributed to C Y Lin.

At least 487 records · Page 27Linked to original sources
PubMed1971

Dissociation of N(2) Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea.

The dissociation of N(2) gas-induced monomeric ribosomes from the pea root was studied by varying the concentration of KCl (or NH(4)Cl) and MgCl(2) in the presence of dithiothreitol. These monoribosomes were shown to dissociate completely into subunits at 0.5m KCl or NH(4)Cl in the presence of 5 mm MgCl(2). The 40S subunits were more susceptible to structural change in KCl than were the 60S subunits. On the other hand, the 60S subunits appeared to be more labile to NH(4)Cl.The activity of the subunits relative to aminoacyl-tRNA binding and peptide bond formation was investigated using subunits derived from 0.5 m KCl (or NH(4)Cl) in the absence and presence of 5 mm MgCl(2). The 40S subunits were active in aminoacyl-tRNA binding only when dissociated in the presence of MgCl(2). The 40S and 60S subunits combined in the presence of poly U were active in incorporation of (14)C-phenylalanine from (14)C-phenylalanyl-tRNA only when dissociation was achieved in the presence of 5 mm MgCl(2). The KCl-dissociated subunits were much more active in protein synthesis than NH(4)Cl-dissociated subunits.

Journal Article↗
PubMed1967

Absence of a Causal Relationship between Auxin-Induced Growth and Changes in the Content of Ascorbic and Dehydroascorbic Acids in Excised Plant Tissues.

The data reported indicate that the oxidation-reduction balance of the ascorbic acid system is not causally related to the auxin-regulation of cell elongation. There was no shift in the ascorbic acid (AA) to dehydroascorbic acid (DHA) ratio with growth-promoting concentration of auxin in several plant tissues. The AA to DHA ratio was experimentally increased without altering the growth rate. Inhibition of growth by supra-optimal auxin was associated with a decrease in the AA to DHA ratio. Since the AA to DHA ratio was lowered by EDTA treatment without altering growth, it seems unlikely that the decrease in the AA to DHA ratio related to the inhibition of growth by high levels of auxin.

Journal Article↗