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Biomedical subjects

C Y Li

Publications and source records attributed to C Y Li.

At least 325 records · Page 18Linked to original sources
PubMed1988

Pathology of the lymph node and spleen in systemic mast cell disease.

The spleen and lymph node are two of the most common organs involved in systemic mast cell disease (SMCD). However, SMCD infiltrates in the spleen and lymph node have a broad spectrum of morphological patterns which can make it difficult to recognize the diagnosis, especially when specimens are examined from patients in whom SMCD is not suspected. We reviewed the pathological features of 16 spleen and 23 lymph node specimens from 19 patients which represented all available material from a series of 58 Mayo Clinic patients with SMCD. The purpose of this study was to investigate the pathological manifestations of SMCD involvement in the spleen and lymph node and to address difficulties in differential diagnosis. All compartments of the spleen and lymph node were found to be affected by SMCD. SMCD lesions in the spleen were found in a paratrabecular (92%), parafollicular (69%), follicular (15%), and a diffuse red pulp (8%) distribution. In the lymph node, mast cell infiltrates affected the paracortex (88%), the parafollicular region (50%), the follicles (25%), the medullary cords (13%), and the sinuses (6%). Mast cells were frequently found in a perivascular location, and associated eosinophilia was common. Because of the broad spectrum of histological manifestations of SMCD in the spleen and lymph node, a wide range of differential diagnoses is discussed including follicular lymphoma, T-cell lymphoma, monocytoid B-cell hyperplasia and lymphoma, Kaposi's sarcoma, and Langerhans' cell granulomatosis.

Diagnosis, Differential↗
PubMed1987

Senile cardiac amyloidosis with myocardial dysfunction. Diagnosis by endomyocardial biopsy and immunohistochemistry.

Senile cardiac amyloid discovered at autopsy is usually regarded as an incidental finding. However, in immunohistochemical studies of autopsy material, three distinct forms of senile cardiovascular amyloid have been characterized, including a systemic form that diffusely infiltrates the cardiac ventricles. The systemic form can be identified immunohistochemically with use of antiserum to human prealbumin. We diagnosed senile systemic amyloidosis causing cardiac dysfunction in five men (57 to 72 years old) by using antiserum to prealbumin in myocardial biopsy tissue. Clinically, the five patients were indistinguishable from patients with nonsecretory immunoglobulin-derived primary amyloidosis with cardiac involvement; only immunohistochemical staining of myocardial tissue distinguished between the two entities. This distinction is important, because the treatment and prognosis of the two disorders are different. We recommend immunohistochemical staining of myocardial tissue for prealbumin in patients with biopsy-proved cardiac amyloid in whom no monoclonal immunoglobulin light chain is detectable in the serum or urine.

Aged↗
PubMed1987

Megakaryoblastic transformation of chronic granulocytic leukemia.

Three cases of megakaryoblastic transformation of chronic granulocytic leukemia (CGL) are reported. In each case, the leukemic transformation had morphologic features suggesting megakaryocytic differentiation. This was confirmed by positive immunostaining with a monoclonal antibody (HP1-1D) specific for platelet and megakaryocyte glycoprotein IIb/IIIa antigen, which was expressed by the majority of the leukemic blasts in all three cases. Cases with evidence of multilineage differentiation of the leukemic transformation were excluded. A striking feature in two patients was the manifestation of lytic bone lesions and soft tissue masses at presentation. A biopsy of a lytic bone lesion and soft tissue mass in one patient revealed a megakaryoblastic leukemic infiltrate, which by immunocytochemical staining was positive for the megakaryocytic markers, glycoprotein IIb/IIIa antigen, and Factor VIII (von Willebrand factor) antigen. In contrast to granulocytic sarcomas, the megakaryoblastic sarcoma did not stain cytochemically for chloroacetate esterase. The mean survival after acute transformation was 5.3 months. The three cases of megakaryoblastic transformation represented a significant proportion of all CGL blastic transformation cases (ten cases) evaluated by bone marrow examination in our institution during a 13-month period. Megakaryoblastic transformation of CGL may occur more frequently than has been appreciated, and can present as lytic bone lesions or as soft tissue megakaryoblastic sarcomas.

Adult↗
PubMed1987

Acute lymphocytic leukemia: correlation of clinical features with immunocytochemical classification.

Many immunologic studies of acute lymphocytic leukemia (ALL) during the past decade have demonstrated the close correlation of immunologic phenotypes of ALL subclasses with the clinical presenting features and prognosis. However, the clinical application of conventional immunologic techniques had been very limited because of the requirement of a fresh sample to prepare the mononuclear cell suspensions for study. We studied 81 cases of ALL using immunoperoxidase stain for nuclear terminal deoxynucleotidyl transferase (TdT) and immunoalkaline phosphatase stain for surface markers (using monoclonal antibody J5 for common ALL antigen [CALLA], Leu-1 for pan-T antigen, and B1 for pan-B antigen) on air-dried smears. The cases were classified as common ALL (TdT+, CALLA+, pan-T-, and pan-B-) (41 cases), null-ALL (TdT+, CALLA-, pan-T-, and pan-B-) (19 cases), T-ALL (TdT+, CALLA-, pan-T+, and pan-B-) (nine cases), B-ALL (TdT-, CALLA-, pan-T-, and pan-B+) (six cases), pre-B-ALL (TdT+/-, CALLA+, pan-T-, and pan-B+) (four cases), or pre-T-ALL (TdT+, CALLA+, pan-T+, and pan-B-) (two cases). This subtyping of ALL correlated well with known clinical presenting features, prognosis, chromosome analysis in 35 cases with an abnormal clone, and conventional immunologic typing in 38 cases. The data suggest that these simple and practical immunocytochemical stains can be used for immunologic subclassification of ALL.

Chromosome Aberrations↗
PubMed1987

Synovial fluid analysis for diagnosis of amyloid arthropathy.

To detect the presence of amyloid, we studied synovial fluid samples from 7 patients who had biopsy-proven amyloidosis and clinical arthropathy and from 98 patients who had other forms of arthritis. Centrifuged sediments from synovial fluids of all 7 patients with amyloid and clinical arthropathy were positive on staining with Congo red, whereas the 98 others were negative. Under polarized microscopy, the amyloid appeared as an amorphous material with strong Congo red uptake and typical apple-green birefringence. These findings indicate that analysis of synovial fluid sediments with Congo red may represent a simple, yet sensitive, test for the diagnosis of amyloid arthropathy.

Aged↗
PubMed1987

Effects of patterned backgrounds on responses of lateral geniculate neurons in cat.

In the lateral geniculate nucleus (LGN) of paralyzed cats under urethane anaesthesia, an extensive disinhibitory region (DIR) outside the inhibitory surround of the receptive field (RF) was found in both sustained and transient cells. Its extent is comparable to that of McIlwain's periphery effect (1964). The responses to a light spot, flashed into different parts of the RF center, were used to assess the effect of different background patterns, located over the DIR, on responsiveness and receptive field organization. A straight line background cutting across the RF center led to a marked shrinkage in RF size and to a suppression of the center response. In sustained cells, these influences were not due to the light flux of the background, but were mainly due to the spatial property of the line itself. This was demonstrated by comparing the effect of a straight line background with that of a zigzag line or of distributed dots. The light flux for the different patterns and their spatial weighting was the same, so that they differed from each other solely in their form. A straight line background elicited much stronger suppression of the center response and more marked shrinkage of the RF than if the component dots are dispersed over a wider area, but keeping the radial distances of the individual dots from the RF-center constant. The data suggest that the dispersion of the component dots in different backgrounds plays an important role as response amplitude and RF diameter increase proportional to the dispersive area of the background patterns. For transient cells, all backgrounds used showed similar effects on center responses and RF diameter, indicating that for them it was the light flux of the backgrounds rather than their spatial property that caused the effects.

Action Potentials↗
PubMed1987

Cytochemical characterization of leukemic cells with numerous cytoplasmic granules.

Ten cases in which leukemic cells contained numerous cytoplasmic granules were examined by using a panel of cytochemical reactions. The diagnoses in the 10 cases were mast cell leukemia, chronic basophilic leukemia, and acute myeloid leukemia with basophilic differentiation in one case each, acute promyelocytic leukemia in two cases, acute megakaryoblastic leukemia in two cases, and blastic hairy cell leukemia in three cases. The cytochemical panel consisted of peroxidase, toluidine blue, chloroacetate esterase, aminocaproate esterase, tartrate-resistant acid phosphatase, and immunoalkaline phosphatase for platelet/megakaryocyte-specific antigen. The unusual cytologic features of leukemic cells in cases similar to our 10 cases have caused considerable diagnostic difficulties. In our 10 cases, however, the effective use of cytochemical studies helped to achieve accurate identification of the various types of leukemic cells. We conclude that the intelligent application of cytochemical techniques continues to be useful for the accurate cytodiagnosis of hematopoietic neoplasms.

Adult↗
PubMed1987

Blastic variant of hairy-cell leukemia.

Three patients with hairy-cell leukemia presented with an unusual combination of clinical and cytologic features. Clinical manifestations included constitutional symptoms, progressive left upper abdomen discomfort, and lymphadenopathy. All three had pancytopenia. Bone marrow aspirate was hypercellular, with extensive replacement by abnormal blastic mononuclear cells with a high nuclear/cytoplasmic ratio. Round, oval, or indented nuclei, with reticular chromatin and prominent nucleoli, and basophilic cytoplasm, with abundant large azurophilic granules, were noted. The bone marrow biopsy specimen showed diffuse involvement in two patients and patchy involvement in one, with absence of fibrosis. The abnormal cells were intensely positive by tartrate-resistant acid phosphatase stain. All of the patients responded well initially to splenectomy. One, who presented with multiple chromosomal abnormalities, had a relatively short survival. The other two are alive. One started therapy with chlorambucil 21 months after operation, and the other presented in a hyperleukocytotic phase five years after operation and responded dramatically to 2'-deoxycoformycin.

Aged↗
PubMed1987

Immunocytochemical identification of cells in serous effusions. Technical considerations.

Immunocytochemical methods were evaluated in order to find a practical one for cell identification on cytologic preparation of body fluids. The effects of fixatives and Triton X-100 treatment on the preservation of cell-type-specific antigens and cell morphologic characteristics were also examined. The method using the alkaline phosphatase-antialkaline phosphatase (APAAP) complex as the indicator is recommended because of its high specificity and sensitivity. With this method, currently available and potentially useful monoclonal antibodies were examined, and the antibodies that were useful for the identification of normal and neoplastic cells commonly present in body fluids were selected for practical applications.

Antibodies, Monoclonal↗
PubMed1987

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.

We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.

Azides↗
PubMed1987

Radiological features of systemic mast-cell disease.

Radiological studies were done on 23 patients with systemic mast-cell disease (SMCD). Significant changes occur most often in bones and less commonly in the gastrointestinal tract and other visceral organs. These changes may be related either to tissue infiltration by mast cells, or to the effect exerted on tissues by chemical mediators of the mast cells, although in some instances findings may be coincidental. Because the radiological changes are not unique to SMCD, their main value, in association with the clinical information, is in directing further studies for diagnostic confirmation and in estimating the extent of systemic involvement.

Adult↗
PubMed1987

Dual action of substance P in nociception and pain modulation at spinal level.

In the present study, the activation of the nociceptive dorsal horn neurons in rats by iontophoretic substance P (SP) has been observed. Thus, the role of SP in spinal nociception is further identified. In addition, the inhibitory effects of iontophoretic SP on nociceptive response (C response) of dorsal horn neurons can also be observed even in rats that have undergone dorsal half transection of spinal cord. These inhibitory effects can be partially blocked by pretreatment with iontophoretic bicuculline but not by naloxone. It indicates that the SP-induced inhibitory effects on the nociceptive response may be mainly mediated by the presynaptic inhibition in which gamma-aminobutyric acid (GABA) may be involved. In view of the fact that the inhibitory effect of stimulation of nucleus raphe magnus (NRM) on the nociceptive response of dorsal horn neurons in rats depleted of 5-HT with parachlorophenylalanine (pCPA) can be significantly blocked by iontophoretic SP-antagonist, it is supposed that SP may be involved in descending modulation on spinal pain transmission.

Animals↗
PubMed1987

Clinical significance of tartrate-sensitive and tartrate-resistant acid phosphatase indicated from the study of their biosynthetic mechanism.

The tartrate-sensitive prostatic acid phosphatase, bands 2 and 4, are found in the soluble cytosol, and absent in the polysome of the prostate, while the tartrate-resistant acid phosphatase band 5 is present in the polysome and the soluble cytosol of hairy cells. The mRNA isolated from the prostate catalyzes the incorporation of 3T leucine into a protein different from that of bands 2 and 4. On the other hand, the mRNA isolated from the hairy cells catalyzes the incorporation of 3T leucine into band 5. The different biosynthetic mechanism of these two types of acid phosphatases are discussed in light of their different clinical significance.

Acid Phosphatase↗
PubMed1987

Some observations on dynamic properties of receptive field organization of complex cells in cat visual cortex.

The dynamic properties of discharge fields (DFs) of the complex cells in areas 17 and 18 of cat's brain were observed. The sequence of activation for different areas in the DF indicated a concentric arrangement of latency distribution. The DE organization was proved to be dependent closely on velocity of stimulus movement. Twin- or triple-DFs were found for some cells, each responded selectively to one of the opposite directions of movement.

Animals↗