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Biomedical subjects

C Xu

Publications and source records attributed to C Xu.

At least 289 records · Page 16Linked to original sources
PubMed1995

[Clinical and experimental researches in the inhibition of bile pigment lithogenesis by acupuncture and moxibustion].

Clinical subjects were the patients in whom cholecystectomy and choledochotomy were performed with T tube drainage. Four groups of acupoints were established according to different point prescriptions. Under the strict control of experimental conditions and fixation of electrical acupuncture stimulation parameters, the changes of hepatic bile output were observed in five different stages before, during and after acupuncture, and compared with those in control group not treated by acupuncture. The results showed that electrical acupuncture of Ganshu (Back-Shu point) (GB 18) and Qimen (Front-Mu point) (LI 14) could obviously promote the secretion of hepatic bile. The immediate effect was superior to that in other groups. In a group not treated by acupuncture, hepatic bile output gradually decreased as time went on. In animal experiment, a model of bile pigment lithogenesis was made in guinea pig. The animals were randomly divided into five groups of control, simultaneous acupuncture, simultaneous moxibustion, re-acupuncture after one week and re-acupuncture after two weeks. The results indicated that electrical acupuncture or moxibustion of relevant acupoints such as Ganshu (GB 18) and Qimen (LI 14) could really very effectively inhibit the animal lithogenesis caused by lithogenesis food. A tendency was observed towards that the earlier acupuncture was performed, the better the preventive effect on lithogenesis. In the influence on lithogenesis bile, both acupuncture and moxibustion could reduce the contents of biliary total bilirubin and free bilirubin and the activity of biliary beta-glucuronidase. Acupuncture also produced an effect in lowering hepatic beta-glucuronidase but moxibustion didn't. Hepatohistological observation showed that lithogenous food could also cause fatty degeneration of liver, and moxibustion could markedly inhibit its progress. Both acupuncture and moxibustion didn't remarkably influence the content of serum cholesterol.

Animals↗
PubMed1995

[The mRNA expression of CD2, CD4 and CD8 in peripheral blood lymphocytes from the patients with acute lymphoblastic leukemia and non-Hodgkin's lymphoma].

The paper for the first time reports the detection of the levels of CD2, CD4 and CD8 mRNA expression in peripheral blood lymphocytes from 9 cases with T-acute lymphoblastic leukemia (ALL) and 10 cases with T-non-Hodgkin's lymphoma (NHL) by using RNA dot blotting technique. It was found that the levels of CD2 mRNA in the two groups of patients were significantly higher than those in the normals. The ratios of the CD4 to CD8 mRNA were also increased in patients with NHL. The significance of this study was discussed.

Adult↗
PubMed1994

Heat shock inhibits and activates different protein degradation pathways and proteinase activities in Neurospora crassa.

In Neurospora crassa, heat shock treatment inhibits proteolytic activity. ATP-independent proteinases were analysed after polyacrylamide gel electrophoresis using renaturing gelatine gels. Proteinases of 24, 29, and 130 kDa were shown to be inhibited by heat shock and were further characterized as to their properties. A major part of the heat shock-induced inhibition is probably due to suppression of de novo synthesis of proteinases as deduced from experiments with cycloheximide. During several hours of recovery from heat shock, the inhibition of overall protein degradation and ATP-independent proteinases is reversed. Azocasein assays as well as pulse-chase experiments further showed that ATP-dependent protein degradation is only slightly affected by heat shock. Two ATP-binding proteinases of about 60 and 160 kDa even show an increased activity after heat shock. The degradation rate of heat shock proteins is inhibited by heat shock treatment, indicating that they are degraded by ATP-independent proteinases. Western blot analysis of a approximately 40-kDa degradation product of HSP70 containing its amino terminal portion revealed a reduction in the amount of this peptide after heat shock.

Adenosine Triphosphate↗
PubMed1994

GBR 12909 and 12935 block dopamine uptake into brain synaptic vesicles as well as nerve endings.

GBR 12909 and 12935, commonly used as potent neuronal dopamine uptake blockers, also inhibit dopamine uptake into brain synaptic vesicles. The concentrations required for the latter activity (34-45 nM) are one order of magnitude higher than those required for inhibiting neuronal uptake of dopamine (1-6 nM). In contrast, the two activities differ by three orders of magnitude for cocaine (137 microM versus 0.35 microM). We propose that the vesicular effect of GBR-type dopamine uptake blockers should be taken into account when interpreting in vivo experiments.

Animals↗
PubMed1994

Design of a cryogenic videocamera-recorder and image processing system and calculation of volumes of red cells.

Since Rowe reported that the storage technique for red cells at very low temperatures had been realized successfully, many experts who work in the fields of cryobiology and medicine have turned their attention to this storage technique for tissues and organs. Since the first quantitative cryomicroscope was made successfully about 20 years ago, it has been possible to observe changes in shape and phase. Particularly the image processing technique has laid the foundation for quantitative analysis of the relationship between changes in shape and damage from freezing of cells and cooling rate and storage temperature. In this work, we constructed a system consisting of a cryomicroscope, a videocamera-recorder, and an image processor. We carried out many experiments with red cells in cold storage and have established a model for calculating the volumes of red cells. In our experiments we also dynamically traced the shape changes of cells with various experimental parameters.

Blood Preservation↗
PubMed1994

Fluid wall shear stress measurements in a model of the human abdominal aorta: oscillatory behavior and relationship to atherosclerosis.

Clinically significant atherosclerosis in the human aorta is most common in the infrarenal segment. This study was initiated to test the hypothesis that flowfield properties are closely related to the localization of plaques in this segment of the arterial system. Wall shear stress was calculated from magnetic resonance velocity measurements of pulsatile flow in an anatomically accurate model of the human abdominal aorta. The wall shear stress values were compared with intimal thickening from 15 post-mortem aortas measured by quantitative morphometry of histological cross sections obtained at standard locations. Wall shear stress oscillated in direction throughout most of the infrarenal aorta, most prominently in the distal region. The time-averaged mean wall shear stress (-1.7 to 1.4 dyn/cm2) was lowest near the posterior wall in this region. These hemodynamic parameters coincided with the locations of maximal intimal thickening. Statistical correlation between oscillatory shear and intimal thickness yielded r = 0.79, P < 0.00001. Low mean shear stresses correlated nearly as well (r = -0.75, P < 0.00005). Comparison of our data with surface maps of Sudan Red staining and early lesions as reported by others revealed similar conclusions. In contrast, pulse and maximum shear stresses did not correlate with plaque localization as has been shown for other sites of selective involvement by atherosclerosis (r < 0.345). Simulated exercise conditions markedly changed the magnitude and pattern of wall shear stress in the distal abdominal aorta. These results demonstrate that in the infrarenal aorta, regions of low mean and oscillating wall shear stresses are predisposed to the development of plaque while regions of relatively high wall shear stress tend to be spared.

Adult↗
PubMed1994

Lineage-specific and differentiation-dependent expression of K12 keratin in rabbit corneal/limbal epithelial cells: cDNA cloning and northern blot analysis.

Corneal epithelial cells synthesize an acidic (55 kDa) K12 and a basic (64 kDa) K3 keratin as their major differentiation products during an advanced stage of differentiation. In this paper, we describe the cDNA cloning of rabbit K12 keratin. We used a 36 base pairs (bp) oligonucleotide corresponding to a consensus sequence of many known acidic keratins as a probe to screen a cDNA library of normal rabbit corneal epithelium. Several partial cDNA clones were isolated. Hybrid-selection showed that the 3'keratin chain-specific portion of the cDNA hybridizes with K12 mRNA. A rabbit antiserum raised against the C-terminus of the cDNA-deduced amino acid sequence recognizes, in immunoblotting, the K12 keratin. In situ hybridization showed that K12 mRNA is present in all cell layers of central corneal epithelium, but in only the suprabasal cells of limbal epithelium indicating a parallel expression pattern between K12 and K3. Cultured rabbit corneal epithelial cells initially synthesize K14/K5 keratins, but later when the cells become heavily stratified they synthesize large quantities of K12 and K3 mRNAs, as detected by Northern blotting. Cultured esophageal epithelial cells do not make K12 mRNA confirming the tissue-specificity of K12 expression. Although it has been suggested that conjunctival epithelial cells can trans-differentiate into a bona fide corneal epithelium, we showed here that cultured conjunctival cells do not synthesize significant amounts of K12/K3 mRNAs. These results strongly suggest that conjunctival epithelial cells, whose differentiation can be modulated significantly by the extracellular matrix, form a lineage intrinsically distinct from the corneal/limbal epithelial lineage.

Amino Acid Sequence↗
PubMed1994

The optimization of technological condition in the fermentation process of glutamate by pattern recognition method.

The technological condition in the fermentation process of fermentation glutamate (such as pH value, temperature, ventilation rate, etc.) were optimized by computerized pattern recognition method. The visible optimum region may be found based on the mapping from the multi-dimensional pattern space into a plane. It is then transformed along the reciprocal direction into the original data space using Monte Carlo simulation, so the orientation of optimization and the best combination of all parameters can be determined. A new mathematical model is being proposed based on the experimental evidence in production. The transfer ratio of glucose to glutamic acid, the production capacity and the glutamic acid concentration increase 2.9%, 1.45% and 2.65% respectively by operating this optimization method. The method has been widely extended to factories and has granted in decreasing the expense of raw materials and that of the production cost.

Fermentation↗
PubMed1994

[Epidemiological study on the infections of Mycoplasma and Chlamydia in immoral persons and healthy controls in seven areas of China].

1,284 immoral persons and 1,161 healthy controls, from seven areas of China, were detected for the antibodies to Ureaplasma urealyticum (Uu), Mycoplasma hominis (Mh), and Chlamydia trachomatis (Ct) by indirect hemoagglutination (IHA). The results showed that: (1) in the immoral persons, the positive rates of antibody was the highest in Uu (25.47%), higher in Ct (18.22%), and lower in Mh (8.80%); (2) the levels of antibody to Mh, Uu, and Ct were significantly higher in the immoral persons than in the healthy controls; and (3) the immoral persons were commonly complicated with infections caused by Uu and Ct. It was indicated that the immoral persons were risk population of Mycoplasma and/or Chlamydial veneral diseases, and it must be noted for us controlling veneral diseases.

Adolescent↗
PubMed1994

[The effect of a promoter P28-1 of Bacillus subtilis on Streptomyces differentiation].

A 1.1kb promoter P28-1 was inserted into pUC19. After then, the P28-1 was subcloned into the HindIII-EcoRI sites of the high copy number Streptomyces promoter probe plasmid pIJ4083 containing xy1E reporter gene. This recombinant plasmid was designated as pIJ4498. When pIJ4498 was introduced into Streptomyces coelicolor J1501 protoplasts, transformants conferred a white phenotype, whereas the vector pIJ4083 gave rise to colonies of normal, dark grey appearance which is the same as that of J1501 itself. After confirming pIJ4498 structure with some restriction enzymes, it was also introduced into whiG mutant (C71). Crude enzyme extracts were isolated from J1501/pIJ4498, J1501/pIJ4083 and C71/pIJ4498 respectively, the crude enzyme extract from J1501/pIJ4498 could oxidize catechol (colourless) to 2-hydroxy muconic semialdehyde (yellow colour), but crude enzyme extracts from J1501/pIJ4083 and C71/pIJ4498 could not oxidize catechol to 2-hydroxymuconic semialdehyde. The results indicated that P28-1 might be recognised by sigma whiG RNA polymerase, and activated the xylE reporter gene expression and reduced J1501 sporulation.

Bacillus subtilis↗
PubMed1994

Heat shock response and cytotoxicity in C6 rat glioma cells: structure-activity relationship of different alcohols.

In C6 rat glioma cells, the n-alcohols methanol, ethanol, propanol, and butanol and the aromatic alcohol phenol all induce heat shock proteins (HSPs) of high molecular mass (68, 70, 90, and 110 kDa) when applied for 1 hr. The lowest alcohol concentrations that induce HSP synthesis cause about 20% cell death, as determined by neutral red assay. HSP induction thus occurs at alcohol concentrations close to the highest tolerable dose. The cytotoxicity and the potential of alcohols to induce the synthesis of HSPs increase with chain length and are correlated with the lipophilicity of the alcohols. A clear structure-activity relationship is observed for both parameters. A calculation of the putative membrane concentrations of these alcohols reveals that cytotoxic effects (50% cell death) occur at nearly the same membrane concentration (approximately 0.2 M). This also holds true for the lowest HSP 68-inducing alcohol concentrations, but at a lower concentration (approximately 0.12 M). The activities of major proteinases are affected by both heat shock and alcohols. The effects of alcohols also depend on the lipophilicity of the alcohols. Effective concentrations again are close to the highest tolerable dose. The stress reactions measured in terms of significant changes in HSP synthesis and proteinase activity provide information about the mechanisms by which toxic agents act on the cell.

Alcohols↗
PubMed1993

Identification of a cDNA for a human high-molecular-weight B-cell growth factor.

Proliferation is necessary for many of the phenotypic changes that occur during B-cell maturation. Further differentiation of mature B cells into plasma cells or memory B cells requires additional rounds of proliferation. In this manuscript, we describe a cDNA for a human B-cell growth factor we call high-molecular-weight B-cell growth factor (HMW-BCGF). Purified HMW-BCGF has been shown to induce B-cell proliferation, inhibit immunoglobulin secretion, and selectively expand certain B-cell subpopulations. Studies using antibodies to HMW-BCGF and its receptor have suggested that HMW-BCGF, while produced by T cells and some malignant B cells, acts predominantly on normal and malignant B cells. The HMW-BCGF cDNA was identified by expression cloning using a monoclonal antibody and polyclonal antisera to HMW-BCGF. Protein produced from the cDNA induced B-cell proliferation, inhibited immunoglobulin secretion, and was recognized in immunoblots by anti-HMW-BCGF antibodies. The amino acid sequence of HMW-BCGF deduced from the cDNA predicts a secreted protein of 53 kDa with three potential N-linked glycosylation sites. The identification of this cDNA will allow further studies examining physiologic roles of this cytokine. We propose to call it interleukin 14.

Amino Acid Sequence↗