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Biomedical subjects

C Wolf

Publications and source records attributed to C Wolf.

At least 235 records · Page 13Linked to original sources
PubMed1985

[Intrapulmonary percussion. A new method of respiratory therapy].

Intrapulmonary percussion with a high-frequency jet system is a new method in the treatment of respiratory impairment. It imitates the conventional percussion massage of the thorax by introducing high-frequency gas oscillations (300 impulses per minute) into the tracheobronchial system. In 14 patients with abnormal alveolo-arterial oxygen differences due to bronchial mucostasis ventilation was satisfactorily improved by its application.

Humans↗
PubMed1985

A retroviral myc gene induces preneoplastic transformation of lymphocytes in a bursal transplantation assay.

Treatment of chicken embryos with cyclophosphamide results in ablation of bursal lymphocytes. Bursal follicles can be reconstructed by infusion of embryonic bursal cells. Histologic examination of reconstituting bursal follicles showed that the first lymphocytes to appear were large pyrinophilic lymphoblasts that lined up adjacent to the bursal basement membrane and appeared to serve as progenitors for the differentiation of bursal medullary lymphocytes. When these cells were infected with the avian myelocytomatosis virus HB1 bearing a v-myc oncogene they appeared to home to the region of the bursal basement membrane but failed to differentiate. Instead, they formed structures indistinguishable from the preneoplastic transformed follicles that develop during bursal lymphomagenesis induced by lymphoid leukosis viruses. The DNA from these transformed follicles contained the HB1 v-myc gene but lacked the ability to transform NIH/3T3 mouse cells. Therefore these preneoplastic lesions were induced directly by HB1 myc and did not require the expression of Blym-1 or similar oncogenes. Exploitation of this transplantation technique with the chicken bursa will provide a useful method for assessing the stage-specific activity of oncogenes in vivo.

Animals↗
PubMed1985

Stress and ulceration: medico-legal aspects.

This paper outlines the possibility of acute, and perhaps, fatal, if not diagnosed, stress ulcers precipitated by trauma, surgery, or other potentiating events. It should once again be emphasized that prevention is the best treatment for the stress-induced ulcer, and that this prevention may occur at both a secondary level (defusion of emotive or physical precipitators) and a primary level (reduction of gastric acid and/or bleeding).

Arousal↗
PubMed1985

Ventilatory control in diabetes mellitus.

The ventilatory control system was evaluated in a group of 40 diabetic patients (20 with autonomic neuropathy (AN), and 20 without autonomic neuropathy) and 20 controls. The ventilatory increase in response to transient hypoxia was less in diabetics without AN than in controls, but even weaker in diabetics with AN. This pattern was repeated in the ventilatory response to hypercapnia. There was no correlation between the presence of abnormal respiratory responses and the duration of diabetes.

Adult↗
PubMed1985

[Calcium oxalate stones and hyperoxaluria secondary to treatment with pyridoxilate].

Pyridoxilate is a salt formed from glyoxylic acid and pyridoxine. It has been used therapeutically as an antianoxic drug in the treatment of various arterial complaints. Its use is based theoretically on its ability to block the conversion of glyoxylic acid into oxalic acid. The following cases suggest, however, that pyridoxilate can cause stones. Intraperitoneal injection of glyoxylate in doses of 130 mg/kg will cause oxalate stones in rats. The same effect results from injection of 427 mg/kg pyridoxilate (i.e. an equivalent dose of glyoxylate). In human subjects, intravenous injection of 200 mg of pyridoxilate results in a fourfold increase in the urinary oxalic acid content in the two hours following the injection. Thirteen cases of chronic progressive oxalate stone disease have recently been reported in patients receiving a prolonged course of pyridoxilate at 450 to 600 mg daily. Eight of these patients had no previous history of lithiasis. Oxaluria levels of 80 to 100 mg daily are observed in all cases of lithiasis in patients receiving pyridoxilate. The levels fell after cessation of the pyridoxilate treatment, and reverted to normal (30 mg/24 hours) in all but three patients. These three patients maintained levels of close to 50 mg and all three had a previous history of urolithiasis.

Aged↗
PubMed1985

[Continuous sympathetic blockade in frostbite of the extremities].

Continuous epidural anesthesia, started 6 h after severe cold injury of the extremities and continued for 3 days, resulted in complete clinical remission of the frozen areas of the feet. In contrast, repeated stellate ganglion block, started 72 h after cold injury, failed to prevent the development of necroses in the frozen parts of the hands, so that plastic surgery was required.

Adult↗
PubMed1985

[Hemofiltration in chronic heart failure].

30 patients with severe congestive heart failure (NYHA IV) unresponsive to medical management were treated by continuous hemofiltration (CHF). 57% of patients received arteriovenous CHF and 43% of patients venovenous, machine assisted CHF over 95 +/- 31 hours. A reduction of body edemas was achieved. The removal of body fluid by CHF between 2 and 40 kg led to a reduction of body edemas and short-term clinical improvement. Furthermore CHF treatment induced hemodynamic improvement with a reduction of central venous pressure (18 +/- 6 cm H2O pre CHF vs 8 +/- 4 cm H2O post CHF p less than 0.01) and a reduction of left ventricular filling pressure (22 +/- 6 mm Hg vs 14 +/- 5 mm Hg, p less than 0.01), while the left ventricular ejection fraction remained unchanged. Patients with low serum sodium levels (less than 132 mval/l) benefited most. While 28/30 of patients has short-term clinical improvement between 2 and 8 weeks, 38% of patients had long-term benefits.

Adult↗
PubMed1985

[Iatrogenic oxalic lithiasis caused by pyridoxilate].

Piridoxilate (P) is given in cases of angina pectoris or arteritis. It is an intramolecular association of glyoxylic hemiacetal salts of pyridoxine. Glyoxylate has a membranous protective action; pyridoxine is used for the theoretical purpose of preventing oxidation of glyoxylic acid to oxalic acid. We have observed 12 patients with an active calcium oxalate lithiasis who had been taking P for many years. Hyperoxaluria was present in all patients and decreased significantly when the drug was interrupted. Significant hyperoxaluria was also observed in volunteers after ingestion of P (600 mg per day) or i.v. (200 mg). We have obtained experimental calcium oxalate urinary stones after intraperitoneal injection of P to Wistar rats. Piridoxilate represents an important lithogenic factor.

Aged↗
PubMed1984

Effects of AY 9944 on low density lipoprotein metabolism in cultured human fibroblasts.

Treatment of cultured human fibroblasts with the hypocholesterolemic drug AY 9944 resulted in a marked increase in low density lipoprotein internalization and degradation for concentrations up to 5 X 10(-6)M. Low density lipoprotein binding was less affected. Concentrations above 5 X 10(-6)M resulted in a relative decrease in low density lipoprotein degradation, whereas binding and internalization plateaued. The stimulation of low density lipoprotein internalization took place within the first hours of incubation of cells with the drug, which suggests a direct effect on the cell membrane. Such phenomenon could account at least partially for the hypocholesterolemic effect of the drug, besides its inhibitory effect on 7-dehydrocholesterol reductase.

Cell Line↗
PubMed1984

Binding of a spin-labelled chlorpromazine analogue to calmodulin.

The binding of a spin-labelled derivative of chlorpromazine to calmodulin was investigated by e.s.r. spectrometry. The completion of the spectroscopic changes requires the presence of 4 Ca2+ ions per calmodulin molecule. The influences of various physicochemical factors (pH, ionic strength) are discussed in relation to the nature (hydrophobic and polar) of the interactions that hold the drug-calmodulin complex together.

Calcium↗
PubMed1984

Timing of pubertal growth and maturation in the first Zurich longitudinal growth study.

Definitions and normal age values are given for start, peak and end of the pubertal growth spurt. In spite of a sex difference in chronological age and bone age, general maturity at the start and at the peak of the growth spurt was identical in both sexes, i.e. it occurred at the same stage of skeletal maturation and at the same relative distance from adult height. Normal values for the main steps of sexual maturation are also given. The overall pattern of the growth spurt, skeletal maturation and sexual maturation was strikingly similar in both sexes.

Adolescent↗
PubMed1983

Linoleate incorporation into rat liver membranes phospholipids: effect on plasma membrane ATPase activities and physical properties.

Plasma membrane phospholipids were modified by incubation in the presence of linoleyl-CoA with or without added lysolecithin (LPC) for various length of time. In the absence of LPC, a maximum of 10 nmoles linoleyl-phosphatidylcholine (PC) were synthesized and the ATPase specific activities were not affected whereas in the presence of LPC, when linoleyl-PC synthesis rose from 10 to 80 nmoles, the ATPase activities were decreased. The decrease was similar in the Na,K- or in the Mg-dependent-ATPase and reached maximally 30-40%. LPC by itself did not modify the ATPases. A concomitant decrease in DPH polarization was observed when linoleate was incorporated into phospholipids. We concluded that the decreased ATPase specific activities may be due to an increased fluidity of membranes produced by linoleyl- PC synthesis. We compare this modulation of ATPases by the membrane fluidity with the specific effect of linoleyl- PC species on adenylate cyclase.

Adenosine Triphosphatases↗
PubMed1983

Effects of phenothiazines on low density lipoprotein metabolism in cultured human fibroblasts.

Treatment of cultured human fibroblasts with trifluoperazine or chlorpromazine resulted in a biphasic effect on low density lipoprotein (LDL) catabolism, depending upon the dose. At up to 10(-5) M, a marked increase in LDL binding, internalization and degradation was observed. This phenomenon took place within the first hours of incubation with the drugs, suggesting a direct effect on cell membrane physical characteristics, probably related to the lipophilic properties of phenothiazines. Concentrations above 2 X 10(-5) M resulted in a relative decrease in LDL binding and internalization, and in a dramatic decrease in LDL degradation, which may be related to an inhibition of calmodulin-dependent processes.

Calmodulin↗
PubMed1983

Protein-stimulated enrichment of human platelet membranes in linoleylphosphatidylcholines. Effect upon adenylate cyclase and fluidity.

In order to study the effect of linoleyl enrichment of platelet membranes upon adenylate cyclase activity and membrane fluidity, manipulations of platelet phospholipids are carried out with phosphatidylcholine-loaded high-density lipoproteins (HDL) or phospholipid-exchange protein and phospholipid-cholesterol mixed vesicles. Incubation with HDL does not appear to be valuable for this purpose. On the other hand, phospholipid-exchange protein and mixed vesicles can be used successfully. Phospholipid-exchange protein stimulated 3-fold the spontaneous exchange of 2-linoleylphosphatidylcholine between the vesicles and the platelets. Linoleyl enrichment of platelets by dilinoleylphosphatidylcholine is about 25% and by 2-linoleylphosphatidylcholine is about 45-50%. The unsaturation index remains constant when the enrichment is performed using dilinoleylphosphatidylcholine but it increases with 2-linoleylphosphatidylcholine. Basal and prostaglandin E1-stimulated adenylate cyclase activities are not modified by dilinoleylphosphatidylcholine, while they increase significantly in the case of 2-linoleylphosphatidylcholine. There is no significant variation in diphenyl hexatriene fluorescence polarization parameters, either with dilinoleylphosphatidylcholine or with 2-linoleylphosphatidylcholine.

Adenylyl Cyclases↗
PubMed1983

[Improvement of tolerance of dialysis sessions using bicarbonate baths. Results of a controlled study].

Benefits of using bicarbonate (B) containing dialysates instead of acetate (A) dialysates is still controversial. A single blind study was therefore performed using patients as their own control. Thirteen males and 10 females (mean age 44 years), with a poor dialysis tolerance were included in the study. 14 patients were dialyzed one week with dialysate A (A 38 mmol/l, CO3H 0, Na 140, K 1.5, Ca 1.75, Mg 0.75, Cl 108.5) and then one week with dialysate B (A 9, CO3H 29, Na 140, K 1.5, Ca 1.75, Mg 0.75, Cl 108.5); 9 patients were dialyzed one week with B and then one week with A. Measured osmolarity was 302 mOsm/l for dialysate A and 291 for dialysate B. A Unimat dialysate generator from Bellco was used in both cases. Student paired t tests were used for comparisons. Predialysis mean values of body weight, systolic and diastolic blood pressures, serum Na, K, Ca, P, BUN, and creatinin did not differed significantly with A and B dialysates. Hypotensive episodes (19.6% vs 36.2% p less than 0.05), headaches (5.8% vs 14.5%, p less than 0.05), and vomiting (8.0% vs 18.5%, p less than 0.10) were less frequent with B than with A. Postdialysis blood CO3H, K and P were higher with B than with A (all p less than 0.05). Results confirm, in acute conditions, the better tolerance of bicarbonate dialysis comparatively to acetate dialysis.

Acetates↗
PubMed1982

[Differential diagnostic problems and polygraphic studies in a case of Pickwickian syndrome].

The case report of a male patient with the Pickwickian syndrome is presented. The results of the relevant neurophysiological examinations (polygraphic registration of EEG, electrocardiogram, respiration, thorax excursions, submental and intercostal electromyogram, electrooculogram) give an insight into the pathomechanisms and enable the differential diagnosis to be made between this condition and narcolepsy and also the Kleine-Levin syndrome; respiratory investigations (spirometry, hypercapnic ventilatory response: mouth occlusion pressure) make it possible to differentiate between the Pickwickian and Ondine's curse syndrome. The therapeutic management is discussed.

Adult↗