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C Wittekind

Publications and source records attributed to C Wittekind.

At least 181 records · Page 10Linked to original sources
PubMed1988

High spontaneous premature labor rate in insulin-dependent diabetic pregnant women: an association with poor glycemic control and urogenital infection.

The incidence of spontaneously occurring premature labor in insulin-dependent diabetic pregnancies is unclear, because previous studies have been confounded by a high rate of iatrogenic prematurity. The purpose of this study was to determine, in a large population of insulin-dependent diabetic pregnant women, the rate of spontaneous occurrence of premature labor and the various factors that may affect it. We hypothesized a priori that spontaneously occurring premature labor occurs at a high rate in insulin-dependent diabetic pregnant women, mainly because of poor control of diabetes during pregnancy, and is related to the presence of polyhydramnios and hypomagnesemia. One hundred forty-five insulin-dependent diabetic women undergoing 181 pregnancies were recruited since 1978 in an interdisciplinary prospective study. The goals of glucose control were a fasting blood glucose less than 100 mg/dL and a 90-minute postprandial glucose less than 140 mg/dL. The rate of spontaneous premature labor, 31.1%, was significantly higher (P less than .01) than that in a control population managed by the same obstetricians in similar clinical settings (20.2%). The following variables were not significantly associated with the onset of premature labor: maternal age, parity, gravidity, diabetic class according to White, presence of renal disease or retinopathy, previous elective abortion, chronic hypertension or pregnancy-induced hypertension, cigarette smoking, first-trimester or post-20 weeks' gestation vaginal bleeding, maternal serum magnesium concentration, or polyhydramnios.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1987

Immunohistological analysis of lymphocyte subpopulations infiltrating breast carcinomas and benign lesions.

We characterized different subpopulations of infiltrating mononuclear cells using 8 monoclonal antibodies (MAbs) on serial cryosections of breast tissue from 85 cancer patients and 32 samples of benign lesions, and the ABC technique. In general, lymphocytes were found more frequently and more abundantly in cancerous lesions. The infiltrates consisted mainly of T-cells in close contact with malignant cell-nests. T-helper/inducer cells clearly predominated over T-suppressor/cytotoxic cells in neoplastic tissues, whereas in benign tissues the T-helper/suppressor ratios seemed to be well balanced. While a few MAb Leu-7 (HNK-I)-reactive NK cells were found in the stroma of the breast tumors, none could be identified in the noncancerous lesions. The correlation of these data with histology and tumor stage of patients has been evaluated by a quantitative approach using planimetry in an interactive registration system.

Adult↗
PubMed1987

The influence of the wet-fixed Papanicolaou and the air-dried Giemsa techniques on nuclear parameters in breast cancer cytology: a cytomorphometric study.

The influence of fixation and staining on morphometric nuclear parameters in cytologic samples of breast lesions has been investigated with respect to the wet-fix and the air-dry techniques. Aspiration biopsy material from 55 benign and malignant breast lesions was smeared onto slides and stained with a routine Papanicolaou technique (after wet fixation) or with the Azur B-eosin Y stain (after air drying). Tumors were grouped according to both histological grade and lymph node status. Nuclear area, diameter, and perimeter were measured from each specimen with an image analyzer and correlated to grade and stage of the disease. All nuclear parameters were significantly smaller in wet-fixed slides. The coefficient of variation was smaller in air-dried samples, which allowed better discrimination between different groups. Nuclear perimeter had the highest discriminative power. Unintentional air drying in wet-fixed smears increased the coefficient of variation for all nuclear parameters. Air drying of cytologic smears is recommended for morphometric investigations if chromatin texture is of minor importance.

Azure Stains↗
PubMed1987

Computerized morphometric image analysis of cytologic nuclear parameters in breast cancer.

The value of nuclear morphometry in the preoperative fine needle aspiration (FNA) cytologic diagnosis of mammary lesions was investigated and correlated with the lymph node status of the patients. The subjects consisted of four groups of patients: 49 with invasive ductal carcinomas (18 with no positive nodes, 16 with one to three positive nodes and 15 with four or more positive nodes) and 14 patients with benign lesions. The FNA specimens were smeared onto slides and stained by the May-Grünwald-Giemsa technique. The area, perimeter and maximum diameter of 100 randomly chosen nuclei were both measured with the IBAS image analysis system and semiquantitatively estimated with an eyepiece micrometer. For all three parameters, significant differences were found between benign and malignant lesions. The mean nuclear perimeter allowed the morphometric discrimination between all four groups with statistical significance; nuclear area and maximum diameter did not discriminate patients with invasive carcinoma and one to three positive nodes from those with no positive nodes or more than three positive nodes. Morphometry proved to be far superior to eyepiece measurements with respect to accuracy and reproducibility of the results. The results suggest that nuclear perimeter can be used as an additional parameter not only for the FNA cytologic diagnosis of breast cancer, but also for the estimation of patients' prognosis.

Adult↗
PubMed1986

Localization of CEA, HCG, lysozyme, alpha-1-antitrypsin, and alpha-1-antichymotrypsin in gastric cancer and prognosis.

Carcinoembryonic antigen (CEA), beta-human chorionic gonadotropin (HCG), alpha-1-antichymotrypsin (ACT), alpha-1-antitrypsin (AAT) and Lysozyme (LYS) were traced by immunoperoxidase staining in gastric carcinomas. The immunohistological results were evaluated in relation to histological types (WHO and Laurén), stage of disease, grade and survival time. CEA was demonstrated in 96% of the tumours, HCG in 34%, ACT in 78%, AAT in 42%, and LYS in 71%. Comparing the staining patterns of the antigens and the intensity of staining some differences were notable. Except for signet-ring cell carcinomas, all of which were intensively positive, CEA expression decreased significantly with loss of differentiation. This observation was not seen with the other marker substances. None of the tested markers was characteristic for one particular histological type, nor could they be correlated with the tumour stage or grade. The marker positivity of CEA, ACT and LYS was not related to survival time. For HCG only, a correlation between tissue expression and a restricted survival time was established. Patients with AAT positive carcinomas had a significantly better survival probability.

Carcinoembryonic Antigen↗
PubMed1984

Localization of CEA, beta-HCG, SP1, and keratin in the tissue of lung carcinomas. An immunohistochemical study.

One hundred and twenty seven cases of lung tumors were studied by the immunoperoxidase technique for the presence of CEA and beta-HCG. Twenty-nine of these tumors were additionally stained for keratin and SP1, CEA and SP1 could be demonstrated in 80% of the studied cases, while beta-HCG was found in only 9%. SP1 revealed an almost identical staining pattern to CEA and keratin was found only in squamous cell carcinomas. The tissue positivity of none of these three markers correlated with tumor size, lymphnodal involvement or histological type.

Adenocarcinoma↗
PubMed1984

Immunohistological localization of beta-HCG in breast carcinomas.

As reports about HCG positivity in serum or tissue of patients with breast cancer show divergent results, the present investigation was undertaken to study the frequency of positive staining for beta-HCG in breast cancer tissue and to correlate the staining pattern with the histology of the tumor and stage of disease. With the immunoperoxidase technique we studied 129 cases of breast carcinomas. We obtained a positive staining for beta-HCG in 12%. However, in most of the tumors only very few cells contained beta-HCG. Morphologically the positive cells could not be distinguished from beta-HCG-negative cells. We conclude from our data that until now the beta-HCG staining provides no immediate useful information for the management of patients.

Adult↗
PubMed1982

CEA positivity in sera and breast tumor tissues obtained from the same patients.

It is well known that the concentration of serum CEA correlates best with colorectal carcinomas but less well with other solid tumors. The aim of the present study was to elucidate further the poor relationship between confirmed primary mammary carcinomas and CEA serum levels, 108 of these tumors have hence been studied for their tissular CEA positivity and the results obtained were compared with the CEA serum values found preoperatively in the same patients. Techniques employed were the indirect immunoperoxydase method for the histologic studies and the enzymoimmuno-assay for the serum measurements. The discordance and concordance of the CEA results obtained with the two techniques were analyzed in respect to the histologic types of tumors classified following the ICD-O nomenclature. Our results seem to prove that: 1) although most tumors produce the antigen only a fraction of them releases it, and 2) the most common histologic type of tumor is the one secreting the antigen the less frequently. These observations are apparently independant of the tumor stages T1-T4, N- or N+, M-.

Adenocarcinoma, Scirrhous↗
PubMed1982

Immuno-histochemical localization of tissue polypeptide antigen (TPA) and carcino-embryonic antigen (CEA) in breast cancer. A comparative study.

The presence of Tissue Polypeptide Antigen (TPA) and Carcino-embryonic Antigen (CEA) in serial sections of 32 breast carcinomas and one case of neurofibrosarcoma was demonstrated by immunocytochemistry using the indirect peroxidase technique. The neurofibrosarcoma was negative for TPA and CEA. All 32 specimens of breast cancer were positive for TPA and/or CEA. In 12 cases the staining was strongly positive for both TPA and CEA. In other cases either TPA or CEA was more abundant in the tumor. The overall concordance of staining was 58%, which is in relative agreement with the correlation between values for TPA and CEA in serum from patients with breast cancer. The finding that in addition to the difference between tumors, there was a difference within tumors was interesting. This indicated that TPA and CEA may be synthesized during partly different phases of the proliferative cycle.

Antigens, Neoplasm↗
PubMed1982

[Immunohistological studies for demonstration of carcinoembryonic antigen in benign breast lesions].

The carcinoembryonic antigen (CEA) was studied in paraffin sections of 114 benign breast lesions and in 65 tumor-bearing breasts using the indirect immune peroxidase technique. CEA was observed in 21% of the cases studied. In mastopathies without carcinoma it was found in 64%. The results obtained in the different forms of benign lesions suggest a rising CEA positivity with a growing severity of the mastopathies. The observed higher positivity of tissue CEA can be taken as an expression of a higher cellular proliferative activity in mastopathies as compared to fibroadenomas. No statistically significant differences were seen in mastopathies associated with breast carcinomas as compared to mastopathies without neoplasia.

Adenofibroma↗
PubMed1982

[Comparative immunohistological studies of the localization of the carcinoembryonic antigen in benign and malignant breast tissue].

By means of immunoperoxidase techniques carcinoembryonic antigen (CEA) has been localized first in carcinomas of the gastrointestinal tract and then also in other malignant tissues, such as carcinomas of the female genital tract and the breast. There is still an obvious need for intensive studies of the possible effects which the initial tissue processing, i. e. different fixation procedures may have on the localization results of CEA. By using the two step HRP-labelled antibody technique, we studied comparatively the effect of the most employed fixations on tissues from patients with mammary carcinomas and benign breast lesions: 1. Formalin-fixed and paraplast-embedded tissues 2. Cryostat sections of tissues fixed in ethanol and acetone 3. Parablast embedded tissues fixed by the freeze substitution method. The latter sections were tested whether there was any difference in the CEA localization when using the immunofluorescence or immunoperoxidase staining techniques. We concluded from our studies that routinely fixed paraplast sections, stained by the two step HRP-labelled antibody sandwich technique gave sufficient results for the demonstration and localization of CEA.

Breast Neoplasms↗