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Biomedical subjects

C Williams

Publications and source records attributed to C Williams.

At least 523 records · Page 29Linked to original sources
PubMed1987

The influence of training on endurance and blood lactate concentration during submaximal exercise.

The purpose of this study was to examine the effect of short-term training on maximum oxygen uptake (VO2 max) and two different measures of endurance performance. Endurance was determined for 15 female subjects (7 training, 8 control) as (1) exercise time to exhaustion at 80% VO2 max (T80%) and (2) the highest relative exercise intensity tolerable during a 30-minute test (T30 min), before and after a 6-week training period. In addition, VO2 max and the work rate equivalent to a blood lactate concentration of 4 mmol.l-1 (OBLA) were determined. Maximum oxygen uptake increased by 24% (p less than 0.01) for the training group (TG) and 7% (p less than 0.01) for the control group (CG). Cumulative average work rate (CAWR) during T30 min increased by 25% for the TG while there was no change for the CG. No significant difference was found pre- and post-training in the %VO2 max (estimated from CAWR) at which the TG and CG performed T30 min. Exercise time to exhaustion on T80% increased by 347% (p less than 0.01) and 16% (NS) for the TG and the CG respectively. Good correlations were found between VO2 max and CAWR (W) (pre-training r = 0.84; post-training r = 0.83), OBLA (W) and CAWR (W) (pre-training r = 0.89; post-training r = 0.88) and change in endurance time and the change in submaximal blood lactate concentration (r = 0.70, p less than 0.01). The results of this study suggest that the ability to sustain a high relative exercise intensity is not enhanced following short-term training.

Adolescent↗
PubMed1987

Treadmill performance and selected physiological characteristics of wheelchair athletes.

The purpose of this study was to examine selected physiological characteristics and pushing performance over a distance of five kilometres (5 km) in twelve wheelchair athletes (10 paraplegics and 2 tetraplegics). The physiological tests and 5 km wheelchair time trials were performed on a motorised treadmill adapted for use by wheelchairs. The maximum oxygen uptake (VO2 max) and %VO2 max utilised at a fixed speed equivalent to a blood lactate concentration of 4 mmol.l-1, identified as being of importance in successful able-bodied endurance performance, were also found to correlate highly with 5 km performance in wheelchair athletes. Low correlations were found between performance and mean %VO2 max utilised over the whole distance and between performance and oxygen uptake at a standard speed. The results also showed that the tetraplegics had lower physiological reserves than the paraplegics, with a much reduced maximum heart rate and ventilation. The findings indicate that a combination of physiological characteristics influence pushing performance in wheelchair athletes.

Adolescent↗
PubMed1987

Impaired muscarinic endothelium-dependent relaxation and cyclic guanosine 5'-monophosphate formation in atherosclerotic human coronary artery and rabbit aorta.

The dependence of vascular relaxation on an intact endothelium and the relationship between relaxation and cyclic GMP accumulation were determined in coronary arteries isolated from cardiac transplantation patients with or without coronary atherosclerosis. In nonatherosclerotic arteries, the endothelium-dependent agent acetylcholine produced concentration-related relaxations. In atherosclerotic arteries, endothelium-dependent relaxations were abolished with acetylcholine, partly suppressed with substance P and histamine, and completely preserved with the ionophore A23187. In these arteries, the endothelium-independent agent nitroglycerin remained fully active. Accumulation of cyclic GMP in atherosclerotic strips was suppressed with acetylcholine but unattenuated with A23187 and nitroglycerin. In aortas from rabbits with diet-induced atherosclerosis, there was likewise an impaired cholinergic relaxation and cyclic GMP accumulation in the presence of preserved responses to A23187 and nitroglycerin. The results demonstrate that impaired cholinergic responses in atherosclerotic arteries reflect a muscarinic defect and not an inability of endothelium to release endothelial factor or smooth muscle to respond to it.

Acetylcholine↗
PubMed1987

Brainstem auditory evoked potentials in the fetal sheep, in utero.

We studied brainstem auditory evoked potentials (BAEPs) in 8 fetal sheep in utero, ranging in gestational age from 105 to 142 days gestation (normal term 147 days). We could not elicit BAEPs prior to 117 days of gestation. After this age rapid maturation was seen, with three discernible peaks observed prior to 120 days and five peaks after 120 days. A significant (P less than 0.05) gestational age related fall in peak latencies and interpeak latencies was observed. The rate of stimulus presentation that could be tolerated without significant changes in wavepeak latency or amplitude also increased with advancing gestational age. In older fetuses (greater than 125 days), where a differentiated electrocorticogram (ECOG) was observed, differences were seen in latency and amplitude of several of the BAEP wavepeaks dependent upon the state. In high voltage ECOG states the amplitudes of wave IV and V were significantly (P less than 0.05) greater than in the low voltage ECOG state and the latencies of wave I, II and V were significantly (P less than 0.05) longer in low as compared to high voltage ECOG state. The BAEP, being obtainable over very short periods of time, appears to provide a useful indice of neural maturation for the sheep fetus in utero.

Acoustic Stimulation↗
PubMed1987

The development of the somatosensory evoked potential in the unanaesthetized fetal sheep.

Somatosensory evoked potentials were recorded in utero from 13 chronically instrumented fetal lambs (97 to 148 days of gestation) following electrical stimulation of the upper lip or upper limb. Several clear and reproducible peaks were observed. Following upper lip stimulation, peaks were seen with mean peak latencies of 9, 13.2, 17.8, 21.3, 33.8 and 206 ms at a gestational age of 125 days. Similar peaks, but of slightly later mean latencies, were seen following limb stimulation. These peaks demonstrated significant gestational age related falls in peak latencies (P less than 0.05). Several of the mid to late latency peaks, notably those occurring at 21.3, 33.8 and 206 ms, demonstrated changes (P less than 0.05) in both latency (longer in low voltage) and amplitude (reduced in low voltage) dependent on electrocorticographic state. Rate of stimulus presentation also had a significant effect on both amplitude and latency of several peaks (P less than 0.05) with this effect lessening with advancing gestational age. Evoked potentials can thus be successfully obtained from chronically instrumented fetal lambs and provide a useful indice for studies of neural maturation.

Animals↗
PubMed1986

Inhibition of ACTH action on cultured bovine adrenal cortical cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin through a redistribution of cholesterol.

The conversion of cholesterol to cortisol by cultured bovine adrenal cortical cells is stimulated 6-fold by adrenocorticotropin and is limited by the movement of cholesterol to the mitochondria (DiBartolomeis, M.J., and Jefcoate, C.R. (1984) J. Biol. Chem. 259, 10159-10167). Exposure of confluent cultures to the potent environmental toxicant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (10(-8)M), for 24 h prior to adrenocorticotropin (ACTH) addition decreased the rate of ACTH-stimulated steroidogenesis but did not affect the basal rate. TCDD was more effective against stimulation at 10(-11) M ACTH (4-fold) than at 10(-7) M ACTH (10%), consistent with an increase in EC50 for ACTH. Stimulation of bovine adrenal cortical cells by cAMP was similarly decreased by TCDD. In both cases the effectiveness of TCDD increased with time of exposure to the stimulant. The transfer of cholesterol to mitochondria in intact cells was quantitated by means of the 2-h accumulation of mitochondrial cholesterol in the presence of aminoglutethimide, an inhibitor of cholesterol side chain cleavage. Although cholesterol accumulated in the presence of ACTH (13 to 28 micrograms/mg), pretreatment of cells with TCDD caused a decrease in mitochondrial cholesterol (13 to 8 micrograms/mg). The effect of TCDD was produced relatively rapidly (t1/2 approximately 4 h). In absence of TCDD, the mitochondria of ACTH-stimulated cells also eventually lose cholesterol (after 2 h). It is concluded that TCDD pretreatment may increase the presence of a protein(s) that cause mitochondrial cholesterol depletion when the cells are stimulated by ACTH or cAMP. TCDD-enhanced cholesterol efflux from mitochondria diminishes cholesterol side chain cleavage when mitochondrial cholesterol is sufficiently depleted (after 2-4 h).

Adrenal Cortex↗
PubMed1986

Binding and second messengers of prostaglandins F2 alpha and E1 in primary cultures of rabbit endometrial cells.

Several factors and hormones are thought to play a role in the growth control of endometrial cells. We have shown that prostaglandin F2 alpha (PGF2 alpha) is a growth factor for primary cultures of rabbit endometrial cells grown in serum-free, chemically defined medium and that prostaglandin E1 (PGE1) antagonizes the PGF2 alpha induction of growth (Orlicky et al., 1986). [3H]PGF2 alpha binds to whole cells in a time (optimal approximately 30 min)- and temperature-dependent (optimal 37 degrees C), disassociable (90% disassociable within 30 min), saturable (Kd1 = 4.9 X 10(-8) M, n1 = 1.2 X 10(5) molecules/cell; Kd2 = 2.6 X 10(-7) M, n2 = 3.0 X 10(5) molecules/cell), and specific manner. [3H]PGE1 binds in a time-dependent (optimal 25 min), disassociable (90% disassociable within 10 min), saturable (Kd = 6.4 X 10(-8) M, n = 1.2 X 10(5) molecules/cell), and specific manner. This specific binding of [3H]PGF2 alpha and [3H]PGE1 is down-regulatable by prior treatment of the cultures with unlabeled ligand, and up-regulatable by prior treatment of the cultures with indomethacin to inhibit endogenous PG synthesis. Proteolytic enzyme treatment for 2 min reduces the specific binding of PGF2 alpha by 75%. PGE1 stimulates intracellular cAMP synthesis and accumulation in a time (optimal 10 min)- and concentration (half-maximal stimulation at 10(-6) M)-dependent manner but has no effect on intracellular cGMP. PGF2 alpha has no effect on either intracellular cAMP or cGMP in this system. We describe here for the first time the analysis at a biochemical level of the interaction between two prostaglandins, antagonistic to each other in terms of growth regulation.

Alprostadil↗
PubMed1986

Regulation of inositol phosphate levels by prostaglandins in cultured endometrial cells.

Stimulation of cultured rabbit endometrial cells by one of the rabbit endometrial cell culture proliferation factors, prostaglandin F2 alpha (PGF2 alpha), resulted in a very rapid increase in the intracellular levels of [3H]-inositol triphosphate (IP3), [3H]-inositol biphosphate (IP2), and [3H]-inositol monophosphate (IP1) in cells prelabeled with [3H]-inositol. These increases in inositol phosphate levels were detected in periods of stimulation as short as 30 seconds, reached a maximum by 1 1/2-2 min and declined to control levels by 6-10 min. The stimulation was dose-dependent with maximal increases observed near 10(-6) M PGF2 alpha. The cholinergic agent, carbachol, also led to time and dose-independent increases in IP3. Lithium, cadmium, silver, copper, and zinc ions had no effect either on the breakdown of IP3 or on the accumulation of IP1. In contrast, vanadate at 10(-6) or 10(-5) M did lead to a decrease in the breakdown of IP1 and a concomitant increase in IP1, IP2, and IP3. PGF2 alpha was found previously to induce an increase in rabbit endometrial cell DNA synthesis which was inhibited by concomitant or prior addition of prostaglandin E1 (PGE1). PGE1, in a dose-dependent manner, was found to inhibit the observed IP3 increase by PGF2 alpha at 1 1/2 min of stimulation. PGF2 alpha treated and control cultures did not differ in cAMP or cGMP levels, cellular 45Ca uptake, nor cellular 22Na uptake. We propose that IP3 may be one of the intracellular messenger(s) synthesized following the treatment of rabbit endometrial cell cultures with the proliferation agent PGF2 alpha and that it may play a crucial role with cAMP in growth regulation.

Animals↗
PubMed1986

Peroxidase-antiperoxidase evaluation of human oral squamous cell papillomas.

In human beings, the viral origin of condylomatous lesions of the mouth and cervix, multiple papillomas, and verruca vulgaris of the larynx has been established. The viral origin of papillomatosis in rabbits and dogs has also been documented. The literature, however, shows no conclusive evidence of a viral origin of human oral squamous cell papillomas (SCP). The purpose of this study was to use the peroxidase-antiperoxidase (PAP) staining technique to determine whether human papillomavirus (HPV) antigens exist in serial step sections of human oral SCP. Fifty human oral SCPs were randomly selected from the department files, and tissue sections were subjected to an indirect immunoperoxidase-PAP technique. Evidence of HPV-Type I virus antigens was found in 2 of 50 papillomas studied (2 of 194 tissue sections). This suggests that all oral SCPs are not of viral antigen.

Antigens, Viral↗
PubMed1986

Improving care in nursing homes using community advocacy.

Community organizations have often focused their campaigns for social change on the health care system. While the techniques of Saul Alinsky and to a lesser extent Paulo Friere have provided the theoretical backbone for most community organizing efforts, these campaigns frequently are characterized by a rather eclectic synthesis of different methodologies. The nursing home industry has been one of the most severely publicly criticized components of the American health care system, and during the last decade consumer groups have sought to achieve fundamental improvements in the quality of life and care available to nursing home residents. However, the standard Alinsky and Friere techniques have limitations in organizing efforts directed toward nursing home reform. One case study is presented which describes a relatively successful community organizing project for better care in a San Francisco Bay Area nursing home. The organizing method used in this project was one of community advocacy. This method is described in detail, and the limitations and potentials of community advocacy are analyzed.

Aged↗
PubMed1986

The influence of short-term endurance training on maximum oxygen uptake, submaximum endurance and the ability to perform brief, maximal exercise.

The hypothesis that endurance training impairs sprinting ability was examined. Eight male subjects undertook a 30-s sprint test on a cycle ergometer before and after 6 weeks of cycling training for endurance. Maximum oxygen uptake (VO2 max) and submaximum endurance were determined to evaluate the influence of the training regimen on endurance performance. Endurance was defined as the time to exhaustion at a relative exercise intensity of 85% VO2 max. Maximum oxygen uptake was increased by 18% post-training (3.29 +/- 0.29 1 min-1 versus 3.89 +/- 0.49 1 min-1; P less than 0.01), but endurance at the same absolute work rate as pre-training was increased by more than 200% (32.2 +/- 11.4 min versus 97.8 +/- 27.3 min; P less than 0.01). These improvements were accompanied by changes in the cardiovascular and metabolic responses to standard, submaximum exercise. Despite the improvements in endurance, neither performance during the cycle sprint test nor the increase in blood lactate concentration during the sprint was influenced by endurance training. For short-term cycling training, these findings reinforce the concept of training specificity whilst demonstrating that decrements in sprint performance are not a necessary consequence of improved endurance.

Adult↗
PubMed1986

Human muscle metabolism during sprint running.

Biopsy samples were obtained from vastus lateralis of eight female subjects before and after a maximal 30-s sprint on a nonmotorized treadmill and were analyzed for glycogen, phosphagens, and glycolytic intermediates. Peak power output averaged 534.4 +/- 85.0 W and was decreased by 50 +/- 10% at the end of the sprint. Glycogen, phosphocreatine, and ATP were decreased by 25, 64, and 37%, respectively. The glycolytic intermediates above phosphofructokinase increased approximately 13-fold, whereas fructose 1,6-diphosphate and triose phosphates only increased 4- and 2-fold. Muscle pyruvate and lactate were increased 19 and 29 times. After 3 min recovery, blood pH was decreased by 0.24 units and plasma epinephrine and norepinephrine increased from 0.3 +/- 0.2 nmol/l and 2.7 +/- 0.8 nmol/l at rest to 1.3 +/- 0.8 nmol/l and 11.7 +/- 6.6 nmol/l. A significant correlation was found between the changes in plasma catecholamines and estimated ATP production from glycolysis (norepinephrine, glycolysis r = 0.78, P less than 0.05; epinephrine, glycolysis r = 0.75, P less than 0.05) and between postexercise capillary lactate and muscle lactate concentrations (r = 0.82, P less than 0.05). The study demonstrated that a significant reduction in ATP occurs during maximal dynamic exercise in humans. The marked metabolic changes caused by the treadmill sprint and its close simulation of free running makes it a valuable test for examining the factors that limit performance and the etiology of fatigue during brief maximal exercise.

Adult↗