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Biomedical subjects

C Weber

Publications and source records attributed to C Weber.

At least 361 records · Page 20Linked to original sources
PubMed1980

[Gonarthrosis progression after meniscus operation].

Roentgenological follow-up results of meniscectomized patients were checked 10 years after the first inquiry. It is found that osseous changes after a lesion of the meniscus and removal of the meniscus are developed -- as a rule -- after a 10 to 12 years' period. Thereafter they only seldom show a tendency for a further progression.

Adult↗
PubMed1979

Alterations in translational control mechanisms in Friend erythroleukemic cells during DMSO induced differentiation.

Induction of erythroid differentiation in Friend erythroleukemia cells causes a reduction in the rate of protein synthesis and a prolonged growth cycle of the cells. A study of the relative translational efficiencies (RTE) of viral mRNAs and selected host cell mRNAs revealed unexpected low RTEs of the mRNA coding for the gp70 protein and RTE of the mRNA coding for the p30 protein. The RTEs of viral and of some host mRNAs show significant changes during differentiation. The rate of synthesis of corresponding proteins are altered non-coordinately, indicating transcriptional and/or posttranscriptional regulation of mRNA, as well as amplified regulation at the level of translation of mRNA.

Cell Differentiation↗
PubMed1979

[Enzyme arrangement of various tissues in swine. 2. Studies of postpartum development of crude protein levels as well as activities of various enzymes (GOT, GPT, serine dehydratase, xanthine oxidase, fructose-1,6-diphosphatase) in liver, kidneys and muscle of piglets].

Twenty-eight piglets from three litters were analysed for postnatal development (0 to 6 days of age) of the crude protein levels and activities of various enzymes (GOT, GPT, serine dehydratase, xanthinoxidase, fructose-1,6-diphosphatase) in the supernatant of liver, kidneys, and muscles. Both the weight and crude protein levels of the liver increased after birth, which improved the capability of metabolic regulation. GOT and fructose 1,6-diphosphatase activities in the liver tissue increased over the first days after birth. Serine dehydratase activity was not detectable with regularity. GPT activity in the tissues concerned was low by comparison to GOT and underwent little postnatal change. The activity of xanthinoxidase in the liver tissue tended to go up after birth. ACTH (3 IU/kg live weight) was administered to piglets aged five days and did not increase the activity of GOT and fructose-1,6-diphosphatase in the liver after five hours.

Alanine Transaminase↗
PubMed1979

[Determination and postnatal development of fructokinase activity in swine liver].

Starting from the spectrophotometric method, described in ADELMAN et al., optimal reaction conditions for the measurement of fructokinase (ketohexokinase) in pig liver were systematically studied. It was necessary to increase the concentration of the substrate and further to lower the concentration of ATP for an optimal Mg: ATP-radio of 2:1. Using the optimized method fructokinase activity was determined in pig liver in relation to age, beginning from the last days of pregnancy to puberty. In liver of fetuses and newborn piglets during the first two days of life no or only a minute activity of the fructokinase was recorded. Therefore, the high level of fructose in fetal blood results from the inability of the fetus to metabolize fructose synthezised in the placenta or the fetal organs. At the end of the first week of life the activity of fructokinase was 10 times, after the second week 15-20 times higher than at birth. This high level remains constant during the suckling period and after weaning. For this reason, piglets after the first week of life are able to metabolize fructose and after the third week to form glucose and to release it into circulation. In adult pigs the activity of fructokinase in the liver decreases slightly. It corresponds-as in rat and human-to the elimination rate of experimentally applied fructose from the circulation. Therefore, this enzyme even in pigs is of significant importance for the utilization of fructose.

Aging↗
PubMed1978

Validity of the Wu-Hoak method for the quantitative determination of platelet aggregation in vivo.

Quantitative determinations of platelet aggregation were made by a modified version of the Wu-Hoak-method in venous blood samples from ten healthy volunteers. It was demonstrated that the extent to which aggregates are formed depends on the rate of flow in the needle and on other methodical influences as well as on the platelet count. Accordingly, no definite conclusions concerning aggregation conditions in vivo can be drawn from the results obtained with venous blood samples.

Adult↗
PubMed1978

Quantitative changes in platelet aggregation due to physiological and pathological factors and medication.

Quantitative measurements of platelet aggregation in samples of venous blood were made in altogether 66 healthy volunteers by a modified version of the method of Wu and Hoak. The formation of aggregates was found to be unaffected or at the most barely affected by such factors as sex, age, smoking, fatty food, physical exertion and medication with isoprenaline or ovulation inhibitors. No difference in the number of aggregates formed was noted between 12 control patients and 16 patients with myocardial infarcts or 14 with other conditions predisposing to arterial thrombosis. Anticoagulant therapy had no effect on aggregation. It is suspected that aggregation in vitro due to methodological factors may obscure any quantitative alterations in the formation of aggregation present in vivo.

Adult↗
PubMed1978

Determination of fetal maturity from the measurement of the sedimentary fraction of the amniotic fluid (amniocrit).

A simple technique is described for evaluating foetal maturity. This consists in measuring the sedimentary fraction of the amniotic fluid (amniocrit). The amount of sediment, expressed in percent of the total liquid volume varies between 0.33% at 36 weeks of amenorrhea and about 2.22% at 40 weeks. These values are correspond to arithmetical means. The differences between pre-term and term pregnancy are highly significant (p less than 0.001). The mean at 32--36 weeks of amenorrhea is 0.27, and at 37--41 weeks is 1.45. A close correlation between gestational age and the fraction of sediment in amniotic fluid is shown in Fig. 1. The correlation coefficient was 0.726. This technique of measuring the sediment combines simplicity and reliability and can be employed where minimal resources are available, and in rural hospitals. The feasibility of using this procedure in institutions with limited resources is emphasized.

Amniotic Fluid↗
PubMed1978

[Determination of enzyme activities in the synovial fluid of farm animals].

Reported in this paper are methods for spectrophotometric determination of the activities of lactatedehydrogenase (LDH) and aspartate-aminotransferase (AspAT) and for colorimetric determination of the activity of N-acetyl-beta-glucosaminidase (NAG) in the synovial fluid of farm animals. Optimum conditions are suggested, as a result of these studies, for reactions of those enzymes in synovial fluid. The behaviours of these enzymes in synovial fluid were compared to those in the blood serum of domestic animals and man and exhibited close agreement. The test-kits at present commercially available for applications in human medicine and used in the determination of LDH and AspAT activities are suitable also for the determination of enzyme activities in synovial fluid. Enzymes in synovial fluid are highly responsive and, even smallest amounts of synovial fluid, therefore, are sufficient for positive findings in laboratory diagnosis.

Acetylglucosaminidase↗