Biomedical subjects

C Wang

Publications and source records attributed to C Wang.

At least 937 records · Page 52Linked to original sources

PubMed1995

[A study of c-src gene express product pp60c-src in esophageal carcinoma].

Using the specific monoclonal antibody of c-src gene express product, pp60c-src (McAb 327), we studied immunohistochemically in this experiment 35 cases of the esophageal squamous cell carcinomas and 22 adjacent carcinoma tissues. The positive rates of pp60c-src in the squamous cell carcinomas of the esophaguses, the proliferative and the normal squamous epithelis adjacent to the carcinoma were 65.7% (23/35), 100.0% (15/15), and 57.1% (4/7), respectively. There was significant difference among them statistically (P < 0.05). And the express amount of pp60c-src in the esophageal squamous cell carcinoma was higher than those in the proliferative and normal squamous epithelia adjacent to the carcinoma. The positive rates of pp60c-src of the esophageal squamous cell carcinoma with different differentiation grades I, II and III were 80.0% (8/10), 68.4% (13/19), and 33.3% (2/6), respectively. No significant difference was found among them statistically (P > 0.05). The higher the differentiation grade of the esophageal squamous cell carcinoma, the more the express amount of pp60c-src (P < 0.005). The results suggested that the activation and express of c-src gene be associated with the initiation and development of the esophageal squamous cell carcinoma; the express amount of pp60c-src increased in the process and was associated with the differentiation of esophageal squamous cell carcinoma.

Antibodies, Monoclonal↗

PubMed1995

[Co-carcinogenic effect of crocidolite plus benzo(a)pyrene on the lungs of rats].

Epidemiological reports have indicated that occupational exposure to asbestos and smoking may greatly increase the incidence of lung carcinoma. In this study rats were injected intratracheally with crocidolite or benzo(a)pyrene [B(a)P] only, and crocidolite plus B(a)P in combination. The incidence of lung carcinomas in crocidolite group was 6.4% (3/47), in B(a)P group 10.4% (5/48) and crocidolite plus B(a)P group 46.3% (25/54). Besides, the survival curve and the mean survival time of rats with carcinoma dropped distinctly. Significant difference was shown among the crocidolite plus B(a)P group, crocidolite and B(a)P groups (P < 0.005). The result indicated the synergetic effect of crocidolite and B(a)P on the development of lung carcinoma in rats and the importance of giving up smoking in the people exposed to asbestos. A model of lung carcinoma in rat was successfully prepared for further study in experiment on treatment, prevention and mechanism.

Animals↗

PubMed1995

Percutaneous endovascular embolization of intracerebral arteriovenous malformations. Experience in 72 cases.

Endovascular therapeutic embolization of arteriovenous malformations (AVMs) of the brain was performed in 72 patients between October 1986 and March 1993. From October 1986 to May 1991, 38 patients in this series were treated with isobuty1-2-cyanoacrylate (IBCA) glue. After June 1991, in the remaining 34 patients the embolic materials used included surgical silk (5-0), polyvinyl alcohol (PVA), ethanol, and estrogen. After treatment, 29 patients (40.3%) had complete angiographic obliteration of AVM, 15 (21%) transient neurologic postembolization deficit, and 3 (4%) permanent deficit without death. Embolic agents and procedures are discussed as to their curative effects, safety, normal perfusion pressure breakthrough (NPPB) problem, with silk mixture fluid being considered preferable.

Adolescent↗

PubMed1995

[A new zoonosis--investigation of Gardnerella vaginalis disease of fox. III. Epidemiological investigation].

By epidemiological investigation to Gardnerella vaginalis disease of fox of civil main farms raising foxes, this disease was showed to be susceptible to silver foxes, arctic foxes, red foxes and color foxes, the disease was mostly transmitted by copulation, infected foxes played the leading role in epidemic of the disease. Investigative results of all farms raising foxes showed infection rate of fox groups was 0.9-21.9%, resulting in abortion rate was 1.5-14.7%, empty rate was 3.2-47.5%. Serious harm was revealed to the disease. By serology and causative agent isolation, we had proved that the disease was able to infect feeder and manager, it belongs to zoonosis. Racoondog. mink and canine infect with the disease besides fox. White mouse, big white rat, gopher, guinea pig and rabbit for laboratory are not infected with the disease.

Abortion, Veterinary↗

PubMed1995

[Myocardial damage after high tension electricity injury in rabbits].

The experiment was designed to study the condition of rabbit myocardium after 10,000-Volt high tension electricity injury by using light and electron microscopes. Early change in myocardium was detected and it took a dynamic development. Also, the enzymogram of myocardium was found to be altered parallel with the pathological alteration, apart from significant changes showed in electrocardiograms. The result of the study demonstrated that organic injury occurred in myocardium after high tension electricity injury.

Animals↗

PubMed1995

[Detection of hepatitis C virus RNA and C33c antigen in the liver tissue from hepatitis C virus infection patients with chronic liver disease].

To study the distribution of hepatitis C virus (HCV) in liver tissue and its pathogenesis, nonisotopic in situ hybridization with a digoxin labelled cDNA probe to 5'-NC region of HCV and immunohistochemical study with monoclonal anti-body of anti HCV C33c were performed on the liver tissue from 24 HCV infection patients with chronic liver disease. The results were as follows: In the liver tissue of 17 patients with HCV infection (both ELISA anti-HCV and nested PCR HCV RNA positive in serum), the positivity of HCV RNA and C33c were 82.3% (14/17) and 88.2% (15/17) respectively. In 7 patients with anti-HCV positive only HCV RNA was not detected in the liver tissue, but 4 out of these 7 cases were C33c positive. The specific signals of HCV RNA and C33c were mainly localized in the cytoplasm of hepatocytes. The distribution of the HCV infected hepatocytes was spotty, scattered or diffuse. There was no relationship between the distribution and the level of alanine transaminase or pathologic changes in liver. There were many lymphocytes and monoclear cells infitrating the periphery of HCV-infected liver cells. The results suggested that replication of HCV in the cytoplasm of hepatocytes elicits the host immune response in cells infected with HCV and this is important in the pathogenesis of HCV infection.

Adult↗

PubMed1995

[The anti-tumor efficacy of human recombinant interleukin-2 (rIL-2) in vivo].

Our results suggest that the growth of tumors was significantly suppressed when the high-dose rIL-2 (2 x 10(4)U/day) was injected i.p. in mice bearing subcutaneously (s. c.) transplanted tumors. The inhibition effect on the growth of mouse hepatoma HAC was more potent than that on S180 group. It was found that rIL-2 increased significantly the survival of BALB/C mice bearing HAC tumor (P < 0.001). We have also shown that the adoptive transfer of unfractionated LAK cells plus rIL-2 was more effective than rIL-2 alone. The results also indicated that adherent LAK cells (A-LAK) in combination with rIL-2 had higher antitumor effects as compared to standard LAK cells plus rIL-2.

Animals↗

PubMed1995

A comparative study of imovane and estazolam treatment on sleep disturbances.

Twenty-six patients with sleep disturbances who had completed therapy with hypnotics were observed under single-blind and self-comparable method for 7 days each. We found that patients treated with imovane or estazolam had no statistical differences with respect to sleep time onset, total sleep time, nocturnal awakening, feeling after awakening, dream, drug side effects and sleep quality. Imovane performed better than estazolam in sleep time onset and total sleep time. From this study, imovane can be recommended as a new hypuotic to treat patients with sleep disturbances.

Adolescent↗

PubMed1995

[Observations on damage and regeneration of corneal nerves of rabbit eyes with herpes simplex keratitis].

An experimental study on the damage and regeneration of the corneal sensory nerves in rabbit eyes with herpes simplex keratitis (HSK) was carried out by means of acetylcholine esterase staining and transmission electron microscopy. The results indicate that the diminution of the corneal sensitivity detected clinically in HSK is caused by the damage of the terminals of the sensory nerve plexus in the epithelial and subepithelial layers of the cornea and in the later stage of HSK, the corneal sensory nerves begin to regenerate, that is the basis of the restoration of corneal sensitivity.

Acetylcholinesterase↗

PubMed1995

p53 mutations and prognosis in bladder tumors.

The incidence of loss of heterozygosity on chromosome 17p and p53 gene mutations was assessed in 43 bladder tumor patients. Histological findings, cigarette smoking and prognosis were examined for possible correlation with the presence or absence of loss of heterozygosity on 17p and p53 mutations. Of 20 informative cases 10 (50.0%) showed loss of heterozygosity of 17p13, including 9 (90.0%) with disease beyond stage pT2. The p53 mutations were detected in 20 of 43 patients (46.5%), including 9 (95.0%) with disease beyond grade 2 and 17 (85.0%) with cancer beyond stage pT2. The incidence of p53 gene mutations was not significantly influenced by habitual smoking but G:C to T:A substitutions, often observed in lung cancers, were detected only in mutations from smokers (5 of 10 or 50%, p < 0.05). Groups with and without loss of heterozygosity showed essentially the same results, while significant differences were found for groups with grades 1 and 2 to 3 (p < 0.05) cancer, stages pT1 and pT2 to 4 (p < 0.01) disease, and with and without p53 gene mutations (p < 0.01, Cox-Mantel test). Genetic alternation in chromosome 17p and p53 mutations would, thus, appear to occur more frequently in high grade and invasive bladder tumors. Cigarette smoking may possibly be a determining factor of mutations of the p53 gene in bladder tumors. Our results indicate that an unfavorable prognostic factor may possibly be linked not only to histopathological findings but the presence of a p53 mutation in bladder tumors as well. Accordingly, mutations of the p53 gene may be deeply involved in late events of tumorigenesis and possibly useful as ideal molecular markers for prognosis in bladder tumors.

Adult↗

PubMed1995

Biological effects of carbon ions with medium energy on plant seeds.

The biological effects of 46.6 MeV/u 12C6+ ions on four kinds of plant seeds were studied at the Heavy Ion Research Facility in Lanzhou (HIRFL), Institute of Modern Physics (IMP), Academia Sinica. The results show that germination of the seeds is inhibited by exposure to ions. In root tip cells of irradiated seeds, a great variety of chromosomal aberrations were observed. Sensitivities in terms of inhibition of germination and induction of chromosomal aberrations in the four species are correlated.

Carbon↗

PubMed1995

[Field studies on preventive effect of artemether against infection with Schistosoma japonicum].

From August to October 1994, a field trial of preventive treatment with artemether (Art) was carried out in Minglang District of Yiyang City, Hunan Province, an islet with embankment type endemic area nearby southern Donting Lake region. The residents who frequently contacted with the infested water were selected for study and allocated to the Art group and the control group in reference to paired methods of randomization table. About one month before administration of Art, all residents under study were examined by stool hatching technique after nylon-bag concentration to determine the infection rate of each group, and then treated with praziquantel at a single dose of 40 mg/kg in stool egg-negative residents and 50 mg/kg in stool egg-positive ones. In Art group, the first dose of 6 mg/kg was given in late August, followed by repeated dosing every 15 days for 3 times. Placebo (starch) was given to the study residents in the control group at the same time as in Art group. The efficacy was evaluated by stool examination 25-32 days after the last medication. The results showed that after the preventive administration of Art, 20 out of 365 study residents in Art group revealed stool positive with an infection rate of 5.5%, while in the control group 51 out of 376 study residents showed stool positive with an infection rate of 13.6%. The difference between the two groups was statistically significant. Meantime, two cases of acute schistosomiasis were seen in the control group, but none was observed in Art group. The egg per gram of feces (EPG) determined with the Kato-Katz method was 122 +/- 79 (range 12-192) in Art group and 681 +/- 909 (range 12-2,760) in the control group. No apparent adverse side effect was seen during the treatment with Art, and no abnormal change in liver and renal function was detected after the last medication. The preliminary study indicates that application of the preventive administration of Art in the endemic area during the transmission season may effectively reduce both the infection rate and intensity of schistosomiasis.

Adolescent↗

PubMed1995

[Inhalation of platelet-activating factor induced guinea pig airway hyperresponsiveness: role of eosinophils activation].

Exposure of guinea pigs to aerosols of 200 micrograms/ml platelet-activating factor 24 h later, airway hyperresponsiveness to histamine induced and number of eosinophils and hypodense eosinophils in bronchoalveolar lavage fluid (BALF) increased, comparing with the control group. The number of eosinophils in BALF was corelated with PC20 value in PAF-treated group (r = -0.62, P < 0.05). However, the percentage of hypodense eosinophil in BALF had closer relation to airway responsiveness (r = -0.84, P < 0.01). The content of peroxidase in hypodense eosinophils in BALF for guinea pigs treated by inhalation of PAF was lowered markedly than that in normodense eosinophil (P < 0.05). The result suggested that chemotaxis and activation of eosinophils by PAF might play an important role in airway hyperresponsiveness.

Animals↗

PubMed1995

Characteristics of an established retinoblastoma cell line HXO-Rb44.

PURPOSE: To study the retinoblastoma cell culture and to establish a new retinoblastoma cell line. METHODS: 22 retinoblastomas were cultured by using the method of single cell suspension. Characteristics of the cultured cells were studied in the following programs: tumor cell morphology in vitro, electron microscopic, growth curve, cloning in soft agar, immunohistochemistry, karyotype and tumorigenicity. RESULTS: 22 retinoblastomas were cultured successfully in vitro, only a continued cell line HXO-Rb44 was established (more than 3 years). The characteristics of this cell line are that it grew as a suspension of round cell in graps like clusters in vitro, its population doubling time was 44 hours, and it could be cloned in soft agar. Histopathologic and ultrastructured pictures showed the characteristics of Rb. HXO-Rb44 cell was positive to NSE and negative to GFAP in immunohistochemical staining. A subcutaneous injection of HXO-Rb44 cells produced a retinoblastoma in BALB/C athumic nude mice. CONCLUSIONS: HXO-Rb44 has the characteristics of retinoblastoma and is a new retinoblastoma cell line. It is a useful material for study this tumor both in basic and clinical fields.

Animals↗

PubMed1995

[Research on the different blood types umbilical cord blood hematopoietic progenitors mixed culture in vitro].

Using single layer agar culture method, we make a study on culture of human hematopoietic progenitor cell from umbilical cord blood. The result showed the mean value of the granulocyte macrophage progenitor cells (CFU-GM) from twenty neonates cord blood was 95.6 +/- 36.5/10(6) MNC. We compared the mixed culture of two different blood types neonates cord blood hematopoietic progenitors with single sample and found little difference between them in number and growth characteristics. This may provide a theoretical basis for clinical transplantation of mixed human cord progenitor cells.

Blood Group Antigens↗

PubMed1995

[Histomorphology research of keloid in different regions].

In order to explore the mechanism of keloid and provide scientific basis for clinical treatment, we carried out a study on histomorphologic comparison of keloids of different tissues. Based on the histomorphologic features, a keloid was divided into three different parts: invasive, proliferative and aged parts. Its pathologic process was described as invasive period, proliferative period, stable period and aging period, to provide scientific basis for treatment.

Adolescent↗

PubMed1995

[Hepatectomy under liver normothermic ischemia in primary liver cancer patients associated with cirrhosis].

From Aug 1984 to Aug 1990, under one-shot normothermic interruption of Porta Hepatis, hepatectomy was performed in 115 patients with primary liver cancer (PLC). The incidence of associated cirrhosis was 82.8% with liver function in Grade A in 59.4% of patients and in Grade B in 40.6% of patients according to Pugh's ranking criteria. Hemihepatic vascular occlusion was preferred. Due precautions were taken to prevent postoperative hepatic failure, including continuous low-pressure oxygen inhalation, the use of antioxidants and dexamethasone. Post-operative complications were: Hydrothorax (7.8%), subphrenic abscess (3.5%), bite leakage and gastrointestineal bleeding (0.9%). There was no operative mortality and hospital mortality.

Adult↗

PubMed1995

[Responses of somatostatin, beta-endorphin and dynorphin A to A glucose load in two groups of women with polycystic ovarian syndrome].

OBJECTIVES: To investigate the relationship between elevated luteinizing hormone (LH), hyperinsulinemia and neuropeptides in patients with polycystic ovarian syndrome (PCOS). METHODS: An oral glucose (100g) tolerance test (OGTT) was performed in 15 normally menstruating women (control) and 30 PCOS women with LH/follicle stimulating hormone (FSH) ratio greater than 3 (group 1) and 25 PCOS subjects with the ratio < 3 (group 2). The responses of insulin, somatostatin (SS), beta-endorphin (beta-EP) and dynorphin A (Dyn A) during OGTT were measured by RIA. RESULTS: In basal state, significant negative correlations were found between LH and SS (r = -0.51, P < 0.05) in group 1 and between LH and beta-EP (r = -0.49, P < 0.05) in group 2. During OGTT, PCOS women had a greater beta-EP and Dyn A responses in group 1 and an impaired SS response in group 2 as compared with the control. CONCLUSION: These data suggested lower endogenous SS, higher beta-EP and Dyn A may lead to elevation of LH and insulin secretions in patients with PCOS.

Adult↗