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Biomedical subjects

C Wagener

Publications and source records attributed to C Wagener.

116 records · Page 7Linked to original sources
PubMed1980

[Diagnostic significance of tumour markers in clinical chemistry. Report on the workshop conference of the German society for Clinical Chemistry, held on November 15--17, 1979 in Schloss Auel (author's transl)].

A workshop conference was held on the diagnostic significance of tumour markers in clinical chemistry. The aims of the conference were (1) discussion of the methodological prerequisites for the standardization of tumour marker determinations, and (2) description of those clinical conditions under which the determination of tumour markers should be carried out. The following aspects were discussed in detail: Sampling, comparison of different methods, standardization and quality control, and pathobiochemical aspects. As far as clinical aspects were concerned, emphasis was laid upon the following points: Screening and limits of early diagnosis, evaluation of tumour growth, prognosis, follow-up studies. In addition to oncofetal tumour proteins, hormones, enzymes, and placental proteins were also discussed.

Antigens, Neoplasm↗
PubMed1978

A highly sensitive method for the demonstration of carcinoembryonic antigen in normal and neoplastic colonic tissue.

A highly sensitive method for the immuno-histochemical localisation of carcinoembryonic antigen (CEA) is described. This method is based on the binding of a peroxidase-antiperoxidase complex (PAP) to anti-CEA antibodies by means of an anti-gamma-globulin which reacts with both the anti-CEA and the antiperoxidase antibodies. Using the technique described here, CEA was localised in conventionally processed normal and cancerous colonic tissue. In normal as well as in neoplastic tissues, a CEA-specific staining of cell membranes and cytoplasm was demonstrated. In frozen sections of normal colonic tissue CEA was found even at high dilutions of the first antibody; this indicates the high sensitivity of the method. The applicability of the method to conventionally processed and thereby well preserved tissue specimens opens the possibility to identify CEA by light microscopy even at very low concentrations.

Animals↗
PubMed1978

Comparative studies on the radioimmunological determination of carcinoembryonic antigen in tumour tissue.

Human carcinoembryonic antigen (CEA) was measured in perchloric acid extracts from 9 malignant gastrointestinal tumours, using two different radioimmunological methods (Hoffmann-La Roche and CEA-IRE-SORIN). When standard curves were set up, quantitative differences in the degree of inhibition were found between the standards from the two manufacturers. In the Hoffmann-La Roche assay the CEA binding curves of all tumour extracts ran parallel with the standard. However, when the CEA-IRE-SORIN assay was applied two of the nine tumours exhibited different slopes. In one case, using the CEA-IRE-SORIN method, various parts of the tumour exhibited binding curves with non-parallel slopes. The present results suggest that radioimmunologically heterogeneous CEA occurs in different gastrointestinal tumours as well as in various parts of the same tumour.

Carcinoembryonic Antigen↗
PubMedDate not supplied

Expression of biliary glycoprotein (CD66a) in normal and malignant breast epithelial cells.

Biliary glycoprotein (BGP, CD66a, or C-CAM-1) is a cell adhesion glycoprotein expressed in colon, liver, and hematopoietic tissues. Four major isoforms (a-d) of BGP are expressed in most epithelial tissues by alternative mRNA splicing from a single gene. Since BGP is down regulated in colon cancer and in premalignant colonic adenomas, it has been of interest to study its expression in other tumors. Using immunohistochemistry with a BGP specific antibody, and mRNA analysis by in situ hybridization, RNase protection, and RT-PCR, we show here that BGP is expressed to the same extent in both normal and malignant breast, demonstrating that BGP is not down regulated in breast cancer. In normal breast, BGP expression is confined to the apical surface of ductal and lobular epithelial cells, while in invasive carcinoma of the breast, BGP is expressed throughout the cytoplasm. In situ hybridization shows a specific pattern of BGP expression in both normal and malignant breast epithelium. RNase protection analysis confirms the immunohistochemistry results and shows no quantitative differences between normal and malignant breast. RT-PCR analysis agrees with these results and shows that only 3 of the 4 major isoforms (a, c, d) of BGP are expressed in normal and malignant breast. Since recent studies by Turbide et al (Cancer Res 57: 2781-2788, 1997) have shown that the ratio of murine BGP isoforms may affect tumor suppression in colonic cancer, it is proposed here that the isoform difference between human breast and colon may account for the observed lack of BGP down-regulation in breast vs colon cancer.

Antigens, CD↗