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Biomedical subjects

C W Lawrence

Publications and source records attributed to C W Lawrence.

80 records · Page 5Linked to original sources
PubMed1966

Absorption, transport, and storage of retinyl-15-14C palmitate-9,10-3H in the rat.

Retinyl-15-(14)C palmitate-9,10-(3)H was fed to rats in order to study hydrolysis and reesterification of this ester during digestion, absorption, transportation, and storage. After administration there was a progressive increase in the (14)C/(3)H ratio of the retinyl esters as they moved from intestinal contents to intestinal mucosa, lymph, and liver, which indicates that repeated hydrolysis and reesterification occur during the digestion and assimilation of this ester.

Animals↗
PubMedDate not supplied

New mutations affecting induced mutagenesis in yeast.

Previously isolated mutations in baker's yeast, Saccharomyces cerevisiae, that impair induced mutagenesis were all identified with the aid of tests that either exclusively or predominantly detect base-pair substitutions. To avoid this bias, we have screened 11 366 potentially mutant clones for UV-induced reversion of the frameshift allele, his4-38, and have identified 10 mutants that give much reduced yields of revertants. Complementation and recombination tests show that 6 of these carry mutations at the previously known REV1, REV1 and REV3 loci, while the remaining 4 define 3 new genes, REV4 (2 mutations), REV5 and REV6. The rev4 mutations are readily suppressed in many genetic backgrounds and, like the rev5 mutation, impart only a limited deficiency for induced mutagenesis: it is likely, therefore that the REV4+ and REV5+ gene functions are only remotely concerned with this process. The rev6 mutants have a more general deficiency, however, as well as marked sensitivity to UV and an increased spontaneous mutation rate, properties that suggest the REV6 gene is directly involved in mutation induction. The REV5 gene is located about 1 cM proximal to CYC1 on chromosome X.

Arginine↗