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Biomedical subjects

C W Johnson

Publications and source records attributed to C W Johnson.

At least 55 records · Page 3Linked to original sources
PubMed1977

Erythromycin resistant mutations in Bacillus subtilis cause temperature sensitive sporulation.

All of several hundred erythromycin resistant single site mutants of Bacillus subtilis W168 are temperature senstive for sporulation. The mutants and wild type cells grow vegetatively at essentially the same rates at both permissive (30 degrees C) and nonpermissive (47 degrees C) temperatures. In addition cellular protein synthesis, cell mass increases and cell viabilities are similar in mutant and wild type strains for several hours after the end of vegetative growth (47 degrees C). in the mutants examined, the temperature sensitive periods begin when the sporulation process is approximately 40% completed, and end when the process is 90% completed. At nonpermissive temperatures, the mutants produce serine and metal proteases at 50% of the wild type rate, accumulate serine esterase at 16% of the wild type rate, and do not demonstrate a sporulation related increase in alkaline phosphatase activity. The eryR and spots phenotypes cotransform 100%, and cotransduce 100% using phage PBS1. Revertants selected for ability to sporulate normally at 47 degrees C (spot), simultaneously regain parental sensitivity to erthromycin. No second site revertants are found. Ribosomes from eryR spots strains bind erythromycin at less than 1% of the wild type rate. A single 50S protein (L17) from mutant ribosomes shows an altered electrophoretic mobility. Ribosomes from spo+ revertants bind erythromycin like parental ribosomes and their proteins are electrophoretically identical to wild type. These data indicate that the L17 protein of the 50S ribosomal subunit from Bacillus subtilis may participate specifically in the sporulation process.

Bacillus subtilis↗
PubMed1969

Fabry's disease.

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Adult↗
PubMedDate not supplied

Issues in immunohistochemistry.

Immunohistochemistry is a powerful technique used to localize specific molecules in tissues. Literally thousands of immunoreagents specific for an incredibly diverse number of molecules are commercially available, and some of these immunoreagents will detect minute quantities of target molecule in tissues. However, like any powerful technique, the staining study design must be carefully conceived and controlled to avoid misinterpretation.

Animals↗
PubMedDate not supplied

Profiles of exercise history and overuse injuries among United States Navy Sea, Air, and Land (SEAL) recruits.

This prospective study examined running history as a risk factor for subsequent overuse injury in Navy Sea, Air, and Land (SEAL) recruits. Recruits preparing to be Navy SEALs undergo 6 months of rigorous physical training programs, which place these recruits at high risk for developing an overuse injury. We assessed the independent variables of age; prior running frequency, duration, and pace; and training surface. Univariate and multivariate estimates of risk were determined for each variable. We observed an incidence of 3.4 overuse injuries per 1000 recruit-days. Assessing the physical activity of the recruits in the 6 months before entrance into basic training, we found that the recruits who ran at a pace slower than 8 minutes per mile and on softer training surfaces were more likely to sustain an overuse injury during basic training, in both univariate and multivariate estimates of risk. Recruits who ran fewer weekly miles and for shorter durations before basic training were also more likely to sustain an overuse injury according to univariate estimates of risk. Our findings suggest that risk of overuse injuries can be reduced by adjusting exercise routines before training. Running on different type surfaces with a gradual increase in speed, duration, and weekly mileage in the period preceding basic training may reduce risk of overuse injury.

Adolescent↗
PubMedDate not supplied

The effect of foot structure and range of motion on musculoskeletal overuse injuries.

The purpose of this prospective study was to determine whether an association exists between foot structure and the development of musculoskeletal overuse injuries. The study group was a well-defined cohort of 449 trainees at the Naval Special Warfare Training Center in Coronado, California. Before beginning training, measurements were made of ankle motion, subtalar motion, and the static (standing) and dynamic (walking) characteristics of the foot arch. The subjects were tracked prospectively for injuries throughout training. We identified risk factors that predispose people to lower extremity overuse injuries. These risk factors include dynamic pes planus, pes cavus, restricted ankle dorsiflexion, and increased hindfoot inversion, all of which are subject to intervention and possible correction.

Achilles Tendon↗