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Biomedical subjects

C Vicente

Publications and source records attributed to C Vicente.

44 records · Page 3Linked to original sources
PubMedDate not supplied

L-usnate-urease interactions: binding sites for the ligand.

L-usnic acid inactivates urease by formation of high molecular weight aggregates which can reached by a maximum of 880 000. L-cysteine partially reverses the inactivation of stimulating the appearance of active high molecular weight polymers. The existence of two class of binding points for L-usnic acid on the urease molecule is proposed, the first showing high affinity for the ligand, related with the loss of activity, and the second, of low affinity, related to polymerization process.

Benzofurans↗
PubMedDate not supplied

Purification and properties of glucosaminephosphate isomerase of Proteus mirabilis.

A glucosamine-P isomerase has been identified in Proteus mirabilis. The 113-fold purified enzyme exhibits a pH optimum of 7.5 with a secondary maximum at 8.5 and a temperature optimum at 37 degrees C. The apparent Km was 13.3 mM for fructose-6-P and 18.8 mM for L-glutamine. Molecular weight of the enzyme has been estimated as 120 000 and the protein can be dissociated in four subunits by SDS-polyacrylamide electrophoresis.

Carbohydrate Epimerases↗
PubMedDate not supplied

L-usnate-urease interactions: binding sites for polymerization.

L-usnic acid inactivates urease through a process which implicates the blockade of--SH groups in parallel to the formation of inactive polymers. Both L-alanine and L-proline partially reverses the inactivation and effectively diminishes the amount of highly polymerized protein. The amino acids also prevent the linkage of L-usnic acid on the sites of low affinity for the ligand, being then related to the sites of polymerization.

Alanine↗
PubMedDate not supplied

[Haemophilus influenzae infections in children less than 5 years of age in the community of Murcia during the 1992-1999 period].

BACKGROUND: Invasive disease due to Haemophilus influenzae has changed substantially since the introduction of the conjugated vaccine. This report studies the incidence and the clinical-epidemiological characteristics of invasive H. influenzae disease in children under five years of age during 1992-94 (before vaccination), 1995-97 (voluntary vaccination) and 1998-99 (obligatory vaccination). PATIENTS AND METHODS: The study was performed by reviewing the clinical histories of 39 patients with H. influenzae isolates from sterile samples, according to microbiology data. The reference population's subgroup of the study, 40,322 children under 5, comprises 60% of the total in our community. RESULTS: The overall incidence of invasive H. influenzae disease was 12.1/100,000 children under 5 and 15.7, 12.4 and 6.2 for the following periods respectively: before vaccination, during voluntary vaccination, and obligatory vaccination. All cases except one of them, were produced by H. influenzae type b and they were seen in children under 3. Meningitis accounted for more than half of the cases (51.3%). Fever was the most frequent sign (38 of 39 cases). Epiglottitis was the cause of the highest average hospital stay (20.8 days). All the patients were treated with cefotaxime, but half were also administered other antibiotics. Sequelae were seen in 7 cases, with no deaths. CONCLUSIONS: The incidence of invasive H. influenzae disease was drastically reduced and no cases were seen in the last year. However, one vaccinated patient suffered a bacteremic etmoiditis. This case and the possibility of infection due to non-b serotypes requires ongoing surveillance for these infections.

Child, Preschool↗