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Biomedical subjects

C Venet

Publications and source records attributed to C Venet.

23 records · Page 2Linked to original sources
PubMed1992

Canine systemic lupus erythematosus. II: Antinuclear antibodies.

The frequency and the specificities of antinuclear antibodies (ANAb) were studied in dogs with systemic lupus erythematosus (SLE) and compared to those found in normal dogs and in dogs with various infectious diseases. Whole ANAb were detected by immunofluorescence. Anti-double-stranded DNA Ab were found in only 2% of SLE dogs, whereas anti-single-stranded DNA Ab were present in 21.4% of SLE dogs and in 26.8% of dogs with infectious disease. Antihistone Ab were frequently observed in SLE dogs (71%) and are essentially directed against trypsin-resistant epitopes of H3, H4 and H2A. The Western blots of nuclear extracts of HeLa cells were recognized mainly by type 1 Ab (30%, reacting with bands of 43, 36, 35, 34, 30 and 27 kDa) and by anti-Sm Ab (12%) associated with anti-RNP Ab. Anti-SSA and anti-SSB Ab were rare.

Animals↗
PubMed1988

Anti-histone antibodies (ELISA and immunoblot) in canine lupus erythematosus.

In the canine systemic lupus erythematosus (SLE), anti-double stranded DNA (ds-DNA) antibodies (enzyme linked immunosorbent assay (ELISA) or indirect immunofluorescence on Crithidia luciliae) are rare whereas anti-histone antibodies are often found: 61.7% with ELISA and 74% with immunoblot. In canine SLE the pattern of anti-histone antibodies on immunoblot is different from anti-histone antibodies in human SLE. Indeed, histone fractions which are most often recognized by the canine antibodies are by order of frequency H3, H4 and H2A, whereas in man this order is H1, H2B then H3. In the diagnostic criteria of canine SLE, we suggest replacing the anti-ds-DNA antibodies by the anti-histone antibodies detected by immunoblot.

Animals↗
PubMedDate not supplied

[Humoral and cellular anticollagen autoimmunity in rheumatic pathology].

Using an immuno-enzymatic technique, the authors studied the production of anti-collagen I and II auto-antibodies in 171 cases of rheumatoid arthritis (RA), 40 cases of systemic lupus erythematosus (SLE) and 21 cases of overriding syndrome. Cellular immunity to native collagen II was tested in a group of 26 patients with RA and 27 control subjects, by studying the production of LIF (Leukocyte Inhibitory Factor) in response to this antigen. The anti-collagen I and II antibodies are present in 15.2% and 17.5% of RA patients respectively and also in 15% and 15.5% of the SLE patients studied. Anti-collagen I antibodies are associated with RA with extra-articular signs and anti-collagen II antibodies are associated with early RA, but no association could be found with the clinical or radiological course of the disease. Cellular reactivity to collagen II was found in 30% of the cases of RA studied and was associated with the early forms of the disease. Complementary studies on the association of certain HLA antigens with cellular or humoral reactivity to collagen should allow a better approach to the clinical relationships of certain cases of chronic inflammatory rheumatism with anti-collagen auto-immunization.

Adult↗