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Biomedical subjects

C Vaishnavi

Publications and source records attributed to C Vaishnavi.

At least 37 records · Page 2Linked to original sources
PubMed1989

Urinary excretion of renal brush-border enzymes in lepromatous leprosy--a preliminary investigation.

Activities of the brush-border enzymes, alkaline phosphatase, maltase, leucine aminopeptidase, and gamma-glutamyl transpeptidase, were measured in urine samples of 25 lepromatous leprosy patients and an equal number of age-matched healthy controls. None of the patients were shown to be suffering from any other systematic disease. The enzymatic activities were shown to be significantly elevated in leprosy patients when compared to controls.

Alkaline Phosphatase↗
PubMed1989

Primary dapsone resistance as assessed by uptake of labelled thymidine by the macrophage resident Mycobacterium leprae.

12 untreated lepromatous leprosy patients were screened for primary dapsone resistance by the uptake of labelled thymidine by macrophage resident M. leprae. There were found to harbour primary dapsone resistant strains of M. leprae and another three partially resistant strains to the drug. This rapid, simple and reliable method should be used routinely to screen leprosy patients, for drug resistance.

Cells, Cultured↗
PubMed1989

Leprosy and hepatitis B surface antigen.

An ELISA technique has been developed to detect HBsAg in the sera of leprosy patients. Out of ninety-two serum samples taken from untreated leprosy patients, 10 samples were positive for HBsAg. The ELISA used in the present investigation is a low cost, reliable and sensitive marker of HBsAg. It is better than lesser sensitive (haemagglutination and counterimmunoelectrophoresis), costly and hazardous (radioimmunoassay) techniques and is therefore recommended for routine use.

Enzyme-Linked Immunosorbent Assay↗
PubMed1989

Lymphocytotoxins in leprosy.

Lymphocytotoxic antibodies (LCAs) were assayed in serum samples from 60 patients of leprosy spectrum before starting multi-drug therapy (MDT). Seventeen healthy volunteers without any history of viral infection provided control samples. Post treatment follow up samples were also included in the study. In all pretreatment sera of LL, BL and BT/TT patients the levels of LCAs were elevated, the figures were 39.05 +/- 23.87, 44.89 +/- 20.74 and 34.16 +/- 17.50 respectively. With treatment a significant fall in LcAs production was observed in all types of leprosy patients.

Adolescent↗
PubMed1988

False-positive reactions with enzyme-linked immunosorbent assay of Mycobacterium tuberculosis antigens in pleural fluid.

The value of enzyme-linked immunosorbent assay (ELISA) for the diagnosis of tuberculous pleural effusion has not been defined. We performed ELISA by a double antibody sandwich technique with anti-BCG antibody in the solid phase to detect Mycobacterium tuberculosis antigen in pleural fluid from 36 patients with pleural effusion (tuberculosis 15, lung cancer 12, miscellaneous 9). Pleural fluids from 12 of the tuberculosis patients, 12 of the cancer patients and one patient in the miscellaneous group had optical densities above the cut-off point. False-positive reactions in patients with lung cancer limit the usefulness of ELISA with conventional anti-BCG antibody for detection of M. tuberculosis antigen.

Adult↗
PubMed1988

Pityriasis (tinea) versicolor in infancy.

Pityriasis (tinea) versicolor is a common disorder of adults. We cared for five infants (four males and one female) with the disease. Diagnosis was confirmed by potassium hydroxide preparation demonstrating the filaments of Malassezia furfur and/or Pityrosporum orbiculare, the yeast form. Three patients had lesions in the neonatal period. The mother of one baby had pityriasis versicolor. Two patients were siblings. One baby had associated atopic dermatitis and two had a positive family history of atopy.

Age Factors↗
PubMed1988

Bacillaemia in leprosy: correlation with slit-skin and nasal smears.

Twenty five multibacillary patients (BL/LL) were studied for bacillaemia. Majority (76%) showed acid fast bacilli in peripheral blood. There was good correlation between bacillary load in peripheral blood and bacteriological index (BI) but poor correlation with morphological index (MI) of skin slit smear and BI/MI of nasal smear.

Adolescent↗
PubMed1988

Effect of in vitro formed immune complexes on macrophage functions of Mycobacterium leprae infected mice.

Nonspecific macrophage functions were studied in Mycobacterium leprae infected and preformed immune complex (IC) administered normal (NI) and thymectomized/irradiated (TRI) mice at different time periods. Uninfected controls given IC were also included. Significant decrease in the chemotaxis, phagocytosis and bactericidal activities of macrophages obtained from infected groups compared to their controls were observed. Phagocytic and chemotactic activities of macrophages were normal but intracellular killing was seen to be depressed in studies conducted in normal and thymectomized immunosuppressed groups (Vaishnavi et al., 1985, Kumar et al, 1987) which were not administered with preformed IC.

Animals↗
PubMed1988

Effect of multidrug therapy on the levels of antibodies to Mycobacterium leprae glycolipid-1 in the leprosy spectrum.

Sera from one hundred and forty five untreated leprosy patients, ten proven cases of active pulmonary tuberculosis and twenty five healthy volunteers not exposed to M. leprae infection were assayed for PGL-1 antibodies. All available follow up samples after multidrug therapy were also assayed. A decline in the level of PGL-1 antibodies were seen in many of the post-treatment samples, giving an indirect assessment of the bacterial load and the prognosis of the disease.

Antibodies, Bacterial↗
PubMed1985

Phagocytic and bactericidal activities of macrophages from Mycobacterium leprae-infected normal and immunosuppressed mice.

Phagocytic and bactericidal activities were studied in Mycobacterium leprae-infected normal (NI) and thymectomized/irradiated (TRI) mice at different time periods. No significant differences were seen in the phagocytic activity for Staphylococcus aureus at 3, 6, and 9 months in the normal infected (NI) and normal control (NC) mice. A slight but significant decrease in the phagocytosis of S. aureus was seen in the TRI as compared to the NI group at 3 months which recovered at 6 months. Phagocytosis of sheep erythrocytes was depressed in the TRI as compared to the NI group at 6 months only. No significant differences in the phagocytosis of latex particles were seen in any of the groups at any time of infection. Bactericidal activity was significantly reduced in the NI group as compared to the NC group and, similarly, in the TRI group as compared to the TRC group at all periods of infection. The M. leprae-infected T/R 900 group (TRI) showed more decrease in bactericidal activity when compared to the normal infected (NI) group.

Animals↗
PubMed1983

Lymphocyte subpopulations in mice infected with Mycobacterium leprae.

T and B cells were quantitated from the spleen of M. leprae infected mice and correlated with bacillary count in the foot pad. Lymphocyte transformation with PHA and M. leprae (armadillo) antigens was also studied during different months of infection. T cell counts dropped gradually but significantly throughout the course of infection. B cells had a concomitant rise up to 6 months and then registered a fall as compared to the initial control figures. Transformation of lymphocytes with PHA fell significantly after 4th month til the end of the experiment, whereas stimulation index for armadillo antigen rose gradually from 3rd month onwards to reach a peak at 6th month and then fell till the end of the experiment with increasing bacterial population whereas B-cell counts showed little change.

Animals↗
PubMed1983

Effect of dapsone (DDS) on T-cells and their response to PHA and lepromin in tuberculoid leprosy patients.

T and B cell counts and their response to PHA and lepromin were studied in 46 patients of polar tuberculoid leprosy (TT), divided into two age and sex matched groups. Twenty five normal healthy controls were similarly investigated. While one group consisted of 26 untreated TT patients, the other included 20 individuals who had taken dapsone, 50 mg daily, for 12 to 14 months. There was significant decrease in the number of T-lymphocytes and their response to PHA and lepromin was lower in the treated, as compared to the untreated patients and normal persons. No significant change was found in the number of B-lymphocytes in any of the groups.

B-Lymphocytes↗
PubMedDate not supplied

Survival of enteropathogenic Escherichia coli exposed to adverse conditions.

Enteropathogenic Escherichia coli (EPEC) was exposed to different adverse conditions for varying period of time to assess its survival under such circumstances. From the results it is extrapolated that EPEC survive for a very long time when shielded from sunlight and after several hours of exposure to UV irradiation.

Escherichia coli↗