Biomedical subjects
Publications and source records attributed to C Vaille.
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1. Acute galactosamine (Gal) hepatitis was induced in rats drinking ad libitum either sodic bicarbonated water of Vichy Grande Grille (GG) or ordinary tap water (OH2). Two series of experiments were performed. 2. In the first series, Gal-induced hepatitis was moderate. Twenty four hours after IP Gal injection, GG treated rats had relative to OH2 treated rats a decrease of bile flow and BSP excretion, and an increase of serum transaminase and bilirubin. Seven days after Gal the liver had returned to normal except for an increase in biliary bilirubin and liver total lipids. 3. In the second series, Gal-induced hepatitis was severe. Twenty four hours after galactosamine administration, an increase in mortality after anesthesia was found in Gal/GG rats. An increase of liver size and total hepatic lipids was also observed, while bile secretion, BSP excretion, cyt P 450 and ARN decreased in Gal/GG rats. Steatosis and inflammatory reactions were more important in Gal/GG than in Gal/OH2 rats. Most parameters came back to their normal levels in two days in Gal/OH2 rats, while 7 days were generally necessary in Gal/GG treated animals. Hepatic DNA kept increasing in Gal/GG animals and was still higher after 7 days, maybe due to a greater inflammatory reaction in the liver, maybe following a stimulated hepatocyte regenerative response. 4. These results indicate that GG water is not an inoffensive salt solution.
Pancreatic secretion and pancreatic enzyme contents were studied in rats given orally methadone for six months (10 to 50 mg . kg-1 . day-1 in drinking water). The main results were as follows: 1. In conditions of acute pancreatic fistula under urethane anesthesia: (a) basal concentration of total protein increased in the juice (+23 p. 100, p less than 0,05). The increase in trypsinogen appeared larger than that of other enzymes; (b) under conditions of maximal stimulation by 2 deoxy-glucose (2 DG), all secretory parameters were increased (protein x 2.7, p less than 0.05; sodium x 1.7, p less than 0.02; bicarbonate x 1.8, p less than 0.05); (c) under conditions of maximal electrical stimulation of the vagus, pancreatic response was unsignificantly increased; (d) dose-response curves to secretin and CCK-PZ were unsignificantly changed. 2. Pancreatic weight was not changed. In the pancreatic tissue: (a) total protein decreased (-10 p. 100, p less than 0.01) as well as amylase (-30 p. 100 p less than 0.001), lipase (-19 p. 100, p less than 0.01) and trypsinogen (-9 p. 100, NS); (b) concentrations of DNA and RNA were unsignificantly increased; (c) histological structure was normal in optical microscopy. 3. Antral gastrin cell numbers were not changed. 4. In conclusion, a decrease in pancreatic enzymatic contents, associated with an increase of total protein in basal secretion and after maximal stimulation by 2DG are consistent with the hypothesis of an increase in synthesis and a decrease in turnover time of exportable proteins in the pancreatic tissue of methadone treated rats. No chronic pancreatitis was observed in these conditions. Endogenous gastrin was probably not involved in pancreatic changes observed.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.