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C T Uyeda

Publications and source records attributed to C T Uyeda.

17 recordsLinked to original sources
PubMed1992

Bacteriologic and endotoxin analysis of salvaged blood used in autologous transfusions during cardiac operations.

Autologous blood transfusion is a common method of reducing the need for heterologous blood transfusion during cardiac operations. Recently we investigated an outbreak of severe, nonsurgical postoperative bleeding among patients undergoing heart operations and receiving intraoperative transfusion of blood from a cell conservation device (Cell Saver System, Haemonetics Corp., Braintree, Mass.). As a result of this investigation, we conducted a prospective study to determine if bacterial or endotoxin contamination of the blood collected in the Cell Saver System and used for reinfusion during heart operations contributes to postoperative bleeding complications. Patients' blood samples were collected immediately before operation, at the end of cardiopulmonary bypass, 1 hour postoperatively, and from the Cell Saver System. All blood samples were cultured for bacteria, and all plasma samples were assayed for endotoxin. Preoperatively all patients having heart operations were without signs of infection, 33 of 37 blood cultures taken were negative, and none of the plasma samples had detectable endotoxin. After discontinuance of cardiopulmonary bypass but before delivery of blood from the Cell Saver System, bacteria and endotoxin were detected in 11 of 36 (30.6%) and five of 35 (14.3%) of the patients' blood samples, respectively. The blood aspirated from the open chest and collected by the Cell Saver System was culture positive in 30 of 31 (96.8%) samples, and seven of 29 (24.1%) contained endotoxin. One of 28 blood samples collected 1 hour postoperatively was culture positive, and five of 25 samples contained endotoxin. Of 61 total microorganisms isolated, 50 (82%) were coagulase-negative staphylococci, four (6.6%) aerobic diphtheroids, five (8.2%) anaerobic "diphtheroids" (Propionibacterium acnes), and two (3.2%) gram-negative bacilli. Plasma endotoxin concentrations ranged from 10 to 765 pg/ml. No signs of endotoxemia or unusual bleeding were observed intraoperatively or postoperatively in any of the 38 patients. Although blood collected in the Cell Saver System and used for reinfusion during heart operations often was contaminated with gram-positive bacterial commensals of the skin and low concentrations of endotoxin, no adverse effects were noted in the patients.

Bacteria↗
PubMed1984

Evaluation of Gonochek-II as a rapid identification system for pathogenic Neisseria species.

The Gonochek-II test kit (E-Y Laboratories, San Mateo, Calif.) may be used to rapidly identify (within 30 min) Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria lactamica, and Branhamella catarrhalis. The kit consists of oxidase swabs, chromogenic substrates, and EY-20 reagent (diazonium salt derivative). A beta-lactamase tube is also included. Fifty-two clinical isolates were tested from cervical (22 isolates), urethral (13), throat (11), rectal (5), and urine (1) sources. All strains were oxidase-positive, gram-negative diplococci isolated on Thayer-Mayer plates incubated at 35 degrees C in 5% CO2 for 18 to 24 h. Each strain was tested by Gonochek-II, RapID NH (Innovative Diagnostics, Decatur, Ga.), and conventional rapid carbohydrate utilization. Forty-four isolates of N. gonorrhoeae and six N. meningitidis were identified. Only two isolates tested (4%) were identified as species other than these two. Gonochek-II outperformed all other methods for identification of N. gonorrhoeae from cervical and urethral areas. N. meningitidis strains were correctly identified in all cases. Ease and rapidity of the procedure, coupled with a small inoculum requirement and reliable results, led us to favor Gonochek-II for routine identification of pathogenic Neisseria species.

Aminopeptidases↗
PubMed1984

Rapid diagnosis of chlamydial infections with the MicroTrak direct test.

A direct test on clinical specimens, using fluorescein-labeled monoclonal antibodies, for Chlamydia trachomatis (MicroTrak [Syva Co.]) was evaluated for the rapid diagnosis of chlamydial infections. Asymptomatic females attending pregnancy or planned parenthood clinics were tested by the direct test and by a cell culture method. Of 401 paired, endocervical specimens, 398 (99.3%) gave identical direct and culture results. The overall sensitivity of the direct test was 96.3% (26 of 27), and the specificity was 99.5% (372 of 374) as compared with that of culture. More than four-fifths of the direct smears were read within ca. 2 min. In this study population, the performance of the direct specimen test was comparable to that of cell culture methods. Rapid turnaround time and elimination of the need for cell culture make the direct test a practical method for the specific diagnosis of chlamydial infections.

Antibodies, Monoclonal↗
PubMed1977

Unidentified gram-negative rod infection. A new disease of man.

A Gram-negative bacillus that defies identification was isolated from blood cultures of 17 patients with fever. Fifteen patients were male adults, and 14 patients had underlying diseases, including previous splenectomy in five, which impair host defenses against infection. Illnesses occurred in the summer and autumn in 14 cases and had been recently preceded by dog bites in 10 cases. Clincal syndromes included cellulitis in seven cases, primary bacteremia without localization in four, purulent meningitis in four, and endocarditis in three. Three patients died. The organism grows slowly on blood or chocolate agar in 10% CO, is oxidase- and catalase-positive, and is negative for nitrate reduction, indole production, and urease. It produces acid from glucose, lactose, and maltose. These features distinguish it from all previously described and classified bacteria. Furthermore, the epidemiologic features of the patients suggest that this organism is an opportunistic invader and may have an animal reservoir in nature.

Adolescent↗