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Biomedical subjects

C Snow

Publications and source records attributed to C Snow.

At least 37 records · Page 2Linked to original sources
PubMed1997

Home health heats up. Survey finds massive change at industry's door.

Modern Healthcare's annual Home Care and Durable Medical Equipment Providers survey found massive change at the industry's door. Intensified government scrutiny has Columbia/HCA Healthcare Corp. putting its $1 billion home-care division on the selling block, and continuing anti-fraud efforts may prompt other hospital systems to step back from the home-care business, too.

Data Collection↗
PubMed1997

Leader of the pack.

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Health Facility Merger↗
PubMed1997

Who's the underdog?

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Catchment Area, Health↗
PubMed1997

Vencor takes on risk.

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Insurance, Long-Term Care↗
PubMed1997

Senior services. Hospitals around the country have seen the future and it is gray.

Hospitals across the country are busy adding services and making investments in new facilities and renovations to serve the exploding senior-services market, which has estimated annual revenues of more than $124 billion when the assisted-living, skilled-nursing and home-care industries are combined. MODERN HEALTHCARE looks at what providers in three diverse major markets are doing to stay competitive.

Aged↗
PubMed1997

Discrimination of late apoptotic/necrotic cells (type III) by flow cytometry in solid tumors.

A method is described for the discrimination of Type III, late apoptotic, and necrotic cells, to improve the accuracy of proliferation and ploidy determinations of breast tumors. We selected an immunological probe, antitubulin antibody, and a DNA specific stain, propidium iodide (PI), both capable of crossing the permeable membranes of Type III, late apoptotic, and necrotic cells. This study utilized MDA-MB-175-VII breast carcinoma cells deprived of oxygen for up to 11 d to simulate intratumoral hypoxia, and 10 human breast tumors and mouse-human breast tumor xenografts disassociated by mechanical or enzymatic means. After 24 h under hypoxic conditions, the MDA cells displayed characteristics associated with both apoptosis and necrosis. Approximately 50% of day 1 cells showed membrane permeability by trypan blue and absence of DNA laddering; however, by day 3-4 characteristic apoptotic DNA laddering by gel electrophoresis was evident. Substantial DNA content loss, further evidenced by a reduction in PI staining and fluorescent microscopy, was obvious by day 5. By day 10, 98% of cells showed no propidium iodide staining by conventional PI live/dead cell gating, but were positive for antitubulin antibody staining. When the study was extended to the analysis of ten tumors, antitubulin antibody showed a range of 78%-96% staining with a median value of 87.5%, while PI staining showed a range of 8%-74% with a median value of 11.5%. This study demonstrates that a large percentage of cells in tumors and hypoxic cell populations have significantly reduced DNA content, such that conventional live/dead cell gating using PI may include many Type III cells as live cells, thus significantly altering data involving multicolor investigations.

Animals↗