Biomedical subjects

C Sharma

Publications and source records attributed to C Sharma.

67 records · Page 4Linked to original sources

PubMed1980

Fecal factors which modify the formation of fecal co-mutagens in high- and low-risk population for colon cancer.

Dietary patterns and fecal mutagens of 2 population groups with a distinct risk for colon cancer, high-risk, non-Seventh-Day Adventists and a low-risk, vegetarian Seventh-Day Adventists, in the New York metropolitan area were studied. The diet histories indicate that the intake of protein was identical in the 2 groups; a greater portion coming from meat in non-Seventh-Day Adventists and from vegetables in the Seventh-Day Adventists. The intake of fat was lower in Seventh-Day Adventists. Fecal samples were extracted with dichloromethane, partially purified on silica and assayed for mutagenic activity using the Salmonella/mammalian microsome mutagenicity test. When tested alone, fecal extracts from both groups were non mutagenic in TA98 and TA100 strains. Fecal extracts of non-Seventh-Day Adventists and Seventh-Day Adventists enhanced the mutagenic activity induced by 2-AAF in TA98 and TA100 (co-mutagenic activity). However, non-Seventh-Day Adventists showed a significantly higher co-mutagenic activity in TA98 than did Seventh-Day Adventists.

2-Acetylaminofluorene↗

PubMed1980

Effect of high-fat, high-beef diet and of mode of cooking of beef in the diet on fecal bacterial enzymes and fecal bile acids and neutral sterols.

The effect of a high-fat, high-beef diet and of method of preparation of beef in the diet on the fecal bile acids and neutral sterols and on the activities of fecal bacterial beta-glucuronidase, cholesterol dehydrogenase and 7 alpha-dehydroxylase was studied in healthy men and women, 24-41 years old, who were consuming a customary mixed-western diet. The experimental diets were high in fat and beef, which was cooked rare, medium or well-done. The sequence of dietary regimen was selected at random and each diet phase lasted for 4 weeks. Individual 24-hour fecal specimens were collected from each volunteer for the final 3 days during each dietary period, including pre- and post-experimental periods while they were consuming a customary mixed-western diet. The mode of cooking beef in the high-fat, high-beef experimental diets had no influence on the fat and protein content of the diets but the fat content of experimental diets was high compared to customary mixed-western diet. Fecal bacterial beta-glucuronidase activity and fecal secondary bile acid and cholesterol metabolite levels were significantly higher during the experimental diet periods but the fecal bacterial activities of 7 alpha-dehydroxylase and cholesterol dehydrogenase were unaffected. The mode of cooking beef in experimental diets had no influence on the fecal bacterial enzymes and on the excretion of fecal bile acids and cholesterol metabolites.

Adult↗

PubMed1979

Effect of endotoxin on hepatic pyruvate kinase activity in normal and diabetic dogs.

The influence of endotoxic administration (0.5 mg/kg) on hepatic pyruvate kinase activity in normal (nondiabetic) and diabetic dogs was investigated. Pyruvate kinase activity was not affected four hours after endotoxin injection in nondiabetic dogs. However, it was stimulated by 288% in dogs that were made diabetic prior to the administration of endotoxin. Since pyruvate kinase activity in diabetic dogs without endotoxin treatment was not affected, these findings suggest that the stimulatory effect of endotoxin on pyruvate kinase activity in diabetic dogs may be associated with insulin deficiency and/or high ambient blood glucose level.

Animals↗

PubMed1979