Search PubMed⌕ Search

Biomedical subjects

C Scully

Publications and source records attributed to C Scully.

At least 523 records · Page 29Linked to original sources
PubMed1987

Detection of human papillomavirus DNA in biopsies of human oral tissue.

We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues.

Biopsy↗
PubMed1987

The expression of cell surface MHC class I heavy and light chain molecules in pre-malignant and malignant lesions of the oral mucosa.

Major histocompatibility complex (MHC) class I antigenic expression was examined on epithelial cell surfaces in normal oral mucosa, non-specific oral keratoses, dysplastic epithelium adjacent to carcinomas and in invasive tumour islands of squamous cell carcinomas, using antibodies to HLA-A,B,C shared determinant antigen and beta 2 microglobulin (beta 2m). HLA-A,B,C antigens were present in the basal and lower spinous cells in normal and dysplastic epithelium and in the non-specific keratoses, but in a minority of carcinomas staining was disorganized and absent at the periphery of tumour islands. beta 2m staining was present in the basal and lower spinous epithelial cells in all tissues; staining was lost progressively with increasing dilution of the primary antibody until it was completely lost in the invasive carcinoma tissue at 1:400, in dysplastic epithelium at 1:800 and in non-specific keratoses at 1:3200; in contrast, staining persisted in normal tissues at greater than 1:3200 anti-beta 2m dilution. Loss of beta 2m staining in the dysplastic epithelial tissues correlated broadly with the degree of cellular atypia. The results suggest that there are decreased concentrations of cell surface beta 2m in potentially malignant and malignant epithelial tissues, with normal concentrations of MHC class I heavy chains.

Carcinoma, Squamous Cell↗
PubMed1987

Nucleic acid probes in the study of latent viral disease.

Despite the apparent complexities of vocabulary and techniques involved in nucleic acid hybridization, these methods should become important new weapons in the pathologist's armoury. The particular strengths lie in the analysis of genes whose protein products defy detection either because they are absent (due, for example, to cellular controls as found in viral latency), to a point mutation, or to gene deletion (as is often the case with cellular oncogenes). However, the mere application of this technology will not solve all diagnostic problems. One must be aware, particularly in applying in situ hybridization to a new system, of the artefactual binding of the probes, and suitable control and duplicate experiments should be performed. In addition, it is vital to verify the identity of the probes and the specificity of the reaction by filter hybridization. The latter procedure may seem unwieldy, but is, in fact, no more complex than the recommended procedures for immunohistochemistry, as discussed by Matthews (this symposium). Sadly, for both hybridization and immunological detection, operator inexperience has often prevented such checks of specificity and as a result many spurious results populate the scientific literature, for example a recent controversy over the detection of Human T cell Lymphotrophic Virus Type 1 in multiple sclerosis patients. With careful application, however, these techniques will permit the detection of viral molecules under conditions where traditional electron microscopy/histology have failed, and may reveal possible viral aetiologies for a range of hitherto non-viral diseases.

Autoradiography↗
PubMed1986

Sources and patterns of referrals of oral cancer: role of general practitioners.

Referrals of patients with oral squamous cell carcinomas to an oral medicine clinic were assessed with regard to the sources, delays, and pattern of referrals from general medical practitioners and general dental practitioners. Slightly more patients were referred by dental practitioners than by medical practitioners, but general medical practitioners were far more likely to see advanced tumours and to request an urgent second opinion or suggest a diagnosis of malignant disease. The greatest delay overall was caused by the patients in seeking advice from their practitioner, particularly those who attended a general medical practitioner. Both groups of practitioners requested a hospital opinion within roughly a month--a reasonable interval. Subsequent delays were minimal. Delays occur mainly because the patients are slow in seeking professional advice and, in general, do not appear to have been reduced over the decade since a previous British study on referral patterns was carried out. This study emphasises the importance of educating patients about oral cancer since it is they who appear to be mainly responsible for the delays in diagnosis. The results also help to dispel the myth that general medical practitioners might be less competent at diagnosis and referral of patients with oral cancer than are dental practitioners, though we are aware of misdiagnoses from both groups.

Adult↗
PubMed1986

Oral herpes simplex infection in bullous pemphigoid.

A case of recurrent oral herpes simplex virus infection complicating bullous pemphigoid is described. The possibility of herpesvirus infection should always be considered in immunosuppressed patients with oral ulceration, including those patients with bullous disorders.

Aged↗
PubMed1986

Cheilitis glandularis: a case affecting the upper lip.

Cheilitis glandularis is a rare disorder characterized by enlarged mucous glands, usually in the lower lip, and possibly predisposing to squamous cell carcinoma. A case affecting the upper lip is presented, which appears to be the first report of the disorder in that site.

Cheilitis↗
PubMed1986

Sjögren's syndrome: clinical and laboratory features, immunopathogenesis, and management.

Sjögren's syndrome may be accompanied by local oral problems such as dry mouth, rampant caries, candidosis, or sialadenitis, but it is a systemic autoimmune disorder with wide repercussions, including a small premalignant potential. This article reviews the clinical and immunopathogenic features, as well as the etiology, of Sjögren's syndrome and discusses the diagnosis and management of oral complications.

Humans↗