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Biomedical subjects

C Schmid

Publications and source records attributed to C Schmid.

392 records · Page 22Linked to original sources
PubMedDate not supplied

[Optical and ultrastructural morphology in hyperparathyroidism during dialysis].

Twenty-six cases of surgically removed parathyroid glands in patients under chronic dialytic treatment were studied. In 19 cases a total parathyroidectomy was performed while in the remaining 7 cases only a subtotal parathyroidectomy was possible. All parathyroid glands (98) showed chief cells hyperplasia, with nodular pattern in 24 patients and diffuse pattern in two cases. A solid pattern was observed in 79 parathyroid glands, a trabecular in 49 and a follicular one in 33. Ultrastructural study was performed on 75 parathyroid glands. Electron microscopy has been useful in the evaluation of functional status of the single cell and in the detection of some morphological features (i.e., presence of intermediate filaments in the cytoplasms, mitochondrial abnormalities, multinucleation), rarely observed in hyperplasia and more frequent in adenoma. No morphologic differences have been found between hyperplastic glands and adenomatous glands. In the 27.2 per cent (out of 44 cases) flow-cytometric DNA analysis showed an aneuploid DNA content.

Adult↗
PubMedDate not supplied

[The use of flow cytometry in the study of parathyroid hyperplasia and adenomas].

To discriminate benign from malignant parathyroid glands lesions is often difficult, because the morphologic features lack absolute diagnostic value. The differential diagnosis between chief cell hyperplasia and parathyroid adenoma is frequently based on physical features such as increased gland weight and number of enlarged glands. A carcinoma is identified by the evidence of local invasion, metastases or recurrence. Nevertheless the lesions classified as benign for lack of histologic features of aggressiveness can show nuclear atypia, increased mitotic figures and broad fibrous bands. Since DNA aneuploidy is present in a great number of human neoplasms and DNA aneuploidy has been suggest to be a marker of malignancy, flow cytometric assessment of ploidy appeared a possible method for rapid and objective distinction between benign and malignant lesions. Flow cytometric DNA content was evaluated on 113 parathyroid glands: the parathyroids were resected from 26 patients with hyperparathyroidism and from 17 patients with adenoma. The analysis was performed on paraffin-embedded specimens according to Hedley with minor modifications. Interpretable histograms were obtained for 103 parathyroids gland (mean CV = 5.3). Aneuploidy was detected in 22.5% of glands; in 12 instances of parathyroid hyperplasia the glands of the same patient showed different DNA Indexes. Cytometric results and morphological features do not correlate as far as aneuploidy and cellular atypia are involved. Although our results fail to show any correlation between morphology of parathyroid cells and DNA content, and abnormal DNA content suggests a careful follow up of these patients.

Adenoma↗
PubMedDate not supplied

[Human dirofilariasis: discovery of a gravid female of Dirofilaria repens in a subcutaneous nodule].

A case of human subcutaneous dirofilariasis occurred in a 53 years old woman from Scafati (Salerno province). It was localized in the left submammary region. The patient was operated 16 years ago for a papillary carcinoma of the thyroid. For this reason a metastasis was clinically suspected. The histological examination revealed the presence of a granulomatous nodule formed around a Dirofilaria repens gravid female in the subcutaneous tissue. The presence of microfilariae in the uterus of the nematode would suggest that also a mature male was present at the same time in the patient, although it was not possible to localise it. Haematic eosinophilia was observed.

Animals↗
PubMedDate not supplied

[Anatomopathologic pictures of chronic pancreatitis].

Morphology of pancreas (either esocrine, either endocrine) was studied in 29 cases of surgically treated chronic pancreatitis (27 cases of chronic calcifying pancreatitis and 2 cases of chronic obstructive pancreatitis). Parenchymal sclerosis in chronic calcifying pancreatitis (CCP) which represents the goal of our study was graded as mild (10 cases), moderate (10) and severe (7). Immunoperoxidase staining (PAP method) for insulin, glucagon, somatostatin, pancreatic polipeptide (PP), vasoactive intestinal polipeptide (VIP) and gastrin, was used to investigate endocrine pancreas. Acinar sclerosis and endocrine damage were closely related. Progression of sclerosis into islet appears to follow vascular pedicles producing a fragmentation into small cell groups as final result. In all cases of moderate or severe sclerosis, A/B cell ratio was increased due to the reduction of insulin positive cells. "Adenoma-like complexes", i.e., apparent concentration of islets, resulting from the loss of the acinar component, were observed in 7 cases with moderate or severe sclerosis. Nesidioblastosis was a prominent feature in all cases but one, with a positivity for insulin in 11 cases, for glucagon in 13, for somatostatin in 6 and for PP in 17. No positivity for gastrin was observed, while VIP was detected in a few ganglia. An increased amount of PP cells in islet and budding from the ducts was noticed and their presence outside the pancreatic head was demonstrable in 4 out of the 7 distal pancreatectomy specimens. Our data confirm the secondary involvement of the endocrine pancreas in the sclerotic acinar process.

Adult↗
PubMedDate not supplied

Bone marrow biopsy in the staging of malignant epithelial tumors.

Bone marrow biopsy was performed routinely on 1,068 patients and 1,268 biopsies were obtained. In 547 epithelial tumors bone marrow involvement was present in 95 patients (17.4%); no metastatic involvement was found in 41 cases of nonepithelial tumors. Bone marrow metastases were present mainly in prostatic (54.1%), breast (26.5%), gastric (18.5%) and lung cancer (7.9%). In 26 cases the primary location was initially unknown; histology was useful in the detection of primary tumors. Histology was compared either with aspiration cytology or with bone X-ray and scan.

Biopsy↗
PubMedDate not supplied

Zonal expression of angiotensin II-AT1-receptor genes in rat kidneys.

This study aimed to examine the relative distribution of ANGII-AT1-receptor gene expression in different zones of the rat kidney, namely the glomeruli, cortex (CO), outer and inner stripes of outer medulla (OSOM and ISOM, respectively) and inner medulla (IM). By RNAse protection we found a ratio for the overall abundance of AT1 mRNA of 1, 0.68, 0.52, 0.86 and 0.46 for glomeruli, CO, OSOM, ISOM and IM, respectively. The intrazonal proportion of AT1a and AT1b mRNA was determined by RT-PCR, which yielded values for the ratio AT1a/AT1b of 1.1, 4.7, 4.4, 3.7 and 1.9 for glomeruli, CO, OSOM, ISOM, and IM, respectively. These findings suggest a zonal heterogeneity in the expression of AT1-receptor genes such that the highest expression is found in glomeruli and ISOM, whilst the papilla shows the lowest level of expression. Moreover, there appears to exist a corticopapillary gradient for the relative expression of AT1a and AT1b-receptor genes such that the preponderance of AT1a-receptor gene expression decreases into a papillary direction. Within the CO glomeruli appear as an isle of AT1b receptors of relatively high expression.

Animals↗
PubMedDate not supplied

[Glycosaminoglycanpolysulfate (GAGPS, Arteparon) for basic therapy of arthrosis. I. Comparative studies, Arteparon forte and physical therapy vs. physical therapy alone].

Glycosaminoglycan polysulphate (GAGPS, Arteparon) is effective as a fundamental therapeutic agent in arthrosis (osteoarthritis). Patients with gonarthrosis and coxarthrosis of activity grade II received intramuscular Arteparon forte injections and physical therapy (group 1, n = 69) or physical therapy only (group 2, n = 46). In group 1 23 patients (33.3%) became free of pain as compared to 3 patients (6.5%) in group 2. No therapeutic success was obtained in 6 patients of group 1 (8.7%) and in 14 individuals (30.4%) of group 2.

Adult↗
PubMedDate not supplied

[Glycosaminoglycanpolysulfate (GAGPS, Arteparon) for basic therapy of arthrosis. II. Comparative studies, Arteparon forte and physical therapy vs. physical therapy alone; late results].

Five to 9 months after a comparative study of 2 groups of patients with coxarthrosis and gonarthrosis, 69 individuals (group 1) receiving Arteparon forte injections and physical therapy and 46 persons (group 2) with physical therapy only, questioneer with specific questions was sent out. From 72 questioneers mailed 55 were returned to the investigators of the study. 53 questioneers were suitable for evaluation: 40 in the group 1 and 13 in group 2. 62.5% of the patients in group 1 described their condition improved as compared to 30.8% in group 2. In 46.1% of the patients in group 2 the condition had deteriorated; this occurred in only 17.5% of group 1.

Adult↗