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Biomedical subjects

C Sanderson

Publications and source records attributed to C Sanderson.

At least 55 records · Page 3Linked to original sources
PubMed1982

Inhibition of corticosterone binding in vitro, in rabbit hippocampus, by chromatin bound aluminum.

Brains of patients with senile dementia of the Alzheimer type and with the dialysis encephalopathy syndrome, exhibit elevated aluminum levels. In Alzheimer's disease and in the experimental aluminum induced encephalopathy, intracellular aluminum is associated with nuclear chromatin. The work reported here was undertaken to test whether chromatin bound aluminum in hippocampus of the rabbit interferes with nuclear binding of corticosterone-receptor complexes. The results showed that the mean binding of corticosterone decreased from 460 +/- 71 fmol/mg DNA in controls, to 343 +/- 81 fmol/mg DNA in hippocampal nuclei from aluminum treated rabbits, representing a decrease of 25%. This reduction occurred in the absence of aluminum induced neurofibrillary degeneration and indicates a possible functional consequence of the presence of aluminum on chromatin, and importantly, in the absence of morphological changes.

Aluminum↗
PubMed1981

Glutamate binding in the rat cerebral cortex during ontogeny.

Two binding sites for L-glutamate have been identified on adult rat brain cortical membranes. One of these sites is Na+-dependent with Kd of 1.3 micro M and a Bmax of 210 pmol/mg protein. The other is Na+-independent with a Kd of 0.37 micro M and Bmax of 6.2 pmol/mg protein. There is a sharp rise in total number of Na+-independent sites per cortex up to 20 days postnatally followed by a more gradual rise to adult levels at 50 days. Na+-dependent binding is also low at birth rising to a peak at 20 days followed by a drop in total levels of binding to 30 days and then a very sharp rise up to 50 days. The kinetics of binding at 20 days gives a Kd for the Na+-dependent site of 1.77 micro M and a Bmax of 82 pmol/mg protein. The Na+-independent site at 20 days has a Kd = 1.3 micro M and Bmax of 8.47 pmol/mg protein. The ability of several acidic amino acid analogues to displace specifically bound L-glutamate was investigated by estimating IC50 values at 20 and 50 days of age. The Na+-independent site is stereospecific for L-glutamate at both ages, but will also interact with L-aspartate at 20 days. The Na+-dependent site has a similar affinity for L- and D-glutamate and L-aspartate at 50 days. The L-glutamate analogue kainate will not displace any bound L-glutamate.

Aging↗
PubMed1978

Characteristic proteins systhesized by suspensions of cells isolated from rat gastric mucosa.

The biochemical integrity of rat gastric mucosal cells after isolation with pronase was demonstrated by their synthesis of a wide range of proteins. Approximately 50 bands of pre-existing proteins were detectable by staining on SDS-polyacrylamide electrophoresis gels. Most bands appeared readiolabelled after incubating cells with 14C-leucine or 14C-proline and were detected by autoradiography and fluorography of the gels. Recent synthesis, indicated by the radioactivity of each band, was compared with historical synthesis indicated by the intensity of staining. Densitometric comparisons showed the relative specific radioactivity of certain bands, e.g., one of nominal molecular weight 25000, to be consistently higher than the integrated mean value.

Animals↗
PubMed1977

Some aspects of management and outcome of acute coronary heart disease in Oxford region.

(1) Over 15 months, 532 consecutive admissions to the CCU at the Radcliffe Oxford were studied; of these 333 were cases of myocardial infarction, and 319 were first admissions for this condition. Information about survival and return to work was collected for 300. A further 30 had artificial pacemakers inserted; there were 141 (26%) of the 532 cases which did not require the special care offered by the CCU. (2) Of 300 patients for whom data were available, 27 were recorded as having received DC shock. In hospital, case fatality was significantly higher among those requiring DC shock than among the remainder. Overall the 3-year survival rates were 47 per cent among those receiving shock, and 62 per cent among the remainder, compared with an expected 91 per cent for a population of the same age and sex. (3) Among men aged under 65 years, 6 of 11 who received shock, compared with 117 (77%) who did not receive shock, returned to work after leaving hospital. (4) Rates of admission to the CCU of cases of myocardial infarction per 1000 standardised population among people living in the areas around Oxford City were estimated as being 58 per cent of admission rates of cases among residents of the city. (5) The case incidence of ventricular fibrillation and the case fatality rate were both higher among those living in the environs than among those living in the city, but these differences were not statistically significant. (6) It is also concluded that insufficient is known about the factors underlying the general practitioner's decision to commit a case of myocardial infarction to other than short ambulance journeys or about the effects of such journeys on prognosis.

Acute Disease↗
PubMed1976

Discussion paper: the use of glutaraldehyde fixation for the study of the immune response to syngeneic tumor antigen.

We have been able to demonstrate that glutaraldehyde fixation of tumor cells or tumor cell membrane fragments protect syngeneic mice against the subsequent challenge with viable cells. To date we have not been able to detect any antibody against the tumor cells in animals so immunized. The specificity of the response is not complete, although a major component of the protective effect of glutaraldehyde-treated tumor cells is immunologically specific.

Aldehydes↗
PubMed1976

Immunoreactive gastrin in differential and density gradient fractions of rat gastric mucosa.

Immunoreactive gastrin was measured in subcellular fractions of rat gastric mucosa. The sedimentational properties of subcellular gastrin-containing structures were distinct from those of mitochondria. After centrifugation in sucrose density gradients using a zonal rotor, the peak of immunoreactive gastrin was found in 1-17--1-18 g cm(-3) density sucrose (1-35 M; 39-5%, W/W). A thermolabile component with 125I-labelled gastrin-binding activity present in gastric mucosal homogenates and fractions was not associated with the gastrin storage vesicles sedimenting in density gradients.

Animals↗