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Biomedical subjects

C Salet

Publications and source records attributed to C Salet.

At least 55 records · Page 3Linked to original sources
PubMed1984

Effects of the mitochondrial probe rhodamine 123 and related analogs on the function and viability of pulsating myocardial cells in culture.

Rhodamine 123, a cationic fluorescent dye, has previously been shown to specifically localize in or on mitochondria in living cells. Since it has also been shown to be relatively non-toxic in a variety of cell types it has been a useful tool for probing mitochondria in vitro. In this report, using cardiac cells in culture, we demonstrate that rhodamine 123 and 6G both positively charged compounds, quickly inhibit beating and kill cardiac-muscle cells while uncharged or neutral rhodamines, 116 and B, produce neither effect. We also present data which illustrate that the cationic rhodamines inhibit oxidative phosphorylation in isolated mitochondria while the neutral dyes do not. It is suggested that both phenomena may be related.

Animals↗
PubMed1983

Effects of photodynamic action on energy coupling of Ca2+ uptake in liver mitochondria.

In mitochondria isolated from rat liver, incubated in the presence of 6 X 10(-3) mM hematoporphyrin and irradiated with UV light at 365 nm, respiration, oxidative phosphorylation and Ca2+ uptake were measured in order to determine the respective photosensitivity of these functions. Irradiation with increasing doses produces uncoupling of oxidative phosphorylation followed by inhibition of Ca2+ uptake and finally arrest of respiration. Ca2+ uptake stimulated by the addition of ATP was also studied in mitochondria uncoupled by irradiation which were still able to concentrate Ca2+ aerobically. Anaerobic Ca2+ uptake driven by ATP hydrolysis was found to be similar in control and in irradiated mitochondria, suggesting a different photosensitivity for the ATPase as compared to the ATP-synthase activity.

Animals↗
PubMed1982

Penetration and localization of furocoumarins in single living cells studied by microspectrofluorometry.

The microspectrofluorometric technique has been used to study the penetration and the localization of psoralen, 4,5',8-trimethylpsoralen and 4'-aminomethyltrioxsalen in single living L-cells. The concentration of the different compounds inside the cell reached a plateau in 2 min with psoralen and aminomethyltrioxsalen and in 20 min with trioxsalen. Washing of the cells with culture medium produced only a partial removal of the three furocoumarins, distributed apparently in equivalent amount in the nucleus and cytoplasm.

Animals↗
PubMed1980

Photophysical properties of aminoethylpsoralen in presence and absence of DNA.

Some properties of the first triplet and singlet excited states of 4'-aminomethyl-4,5',8-trimethylpsoralen have been determined by laser flash photolysis and spectrofluorimetry. In water the lowest triplet has an intrinsic lifetime of 100 microseconds and is quenched by the molecule in its ground state with a rate constant kQ = 10(9) M-1 . s-1 and by thymine with a rate cconstant kq = 2 . 10(8) M-1 . x-1. Addition of 0.5 mg/ml of calf thymus DNA causes the disappearance of the aminomethylpsoralen triplet absorption immediately after the laser flash excitation and extinguishes the fluorescence emission of a 6 . 10(-5) M solution of aminomethylpsoralen. These experiments show that the first singlet excited state which is rapidly deactivated in the presence of DNA could be the precursor of the first pyrimidine photoadduct.

Animals↗