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C S Stulberg

Publications and source records attributed to C S Stulberg.

17 recordsLinked to original sources
PubMed1976

Identification of cells in culture.

Most laboratories using cells cultured in vitro maintain multiple cell lines. Such lines should be monitored for species and intraspecies characteristics to prevent invalidation of research work due to incidents of cell line cross-contamination. This report describes the results obtained when 246 cell cultures were examined for evidence of cross-contamination or mislabeling. Using species-specific antigens, isoenzyme electrophoresis, and chromosomes as markers of identity, 14% of the cultures submitted were found to be contaminated by cells of another species. Of human cell lines submitted 25% were of HeLa cell origin, as determined by 2 intraspecies markers, glucose-6-phosphate dehydrogenase and chromosome analyses. The fact that, overall, nearly 30% of the cell lines examined were incorrectly designated makes the importance of cell line monitoring self-evident.

Animals↗
PubMed1966

Somatic and flagellar immunofluorescence of Salmonella.

Caldwell, W. J. (The Child Research Center of Michigan, Detroit, Mich.), C. S. Stulberg, and W. D. Peterson, Jr. Somatic and flagellar immunofluorescence of Salmonella. J. Bacteriol. 92:1177-1187. 1966.-Labeled globulin fractions of flagellar (H) antisera, prepared against 20 frequently occurring Salmonella serotypes belonging to five major somatic (O) groups, were characterized for O and H immunofluorescence and for O and H agglutinin titers against 32 serotypes. The feasibility of immunofluorescent identification of both somatic and flagellar antigens was enhanced by staining formaldehyde-treated organisms in suspension. Relationships between homologous, partial, and unrelated antigen-antibody systems were then analyzed, and a high degree of correlation was shown between the results obtained by the two serological procedures. Flagellar staining was highly specific, and was bright, faint, or inapparent, depending on the relationship between the antigen-antibody systems involved. Somatic staining was also specific, but somewhat more difficult to interpret, because cells in the same preparation might exhibit a mixture of bright, faint, or no fluorescent intensities. Correlation was shown between the percentage of brightly staining cells found in these preparations and the agglutination titers of the comparable antigen-antibody systems. The phenomenon of a "percentage" reaction was unexplained. Absorption studies further confirmed the specificity of reactions. The techniques developed were applied to surveillance of several mouse colonies for the presence of Salmonella. Broth cultures of fecal specimens were treated with formaldehyde and stained in suspension with "polyvalent" labeled antibody reagents. Agreement was found in 97.6% of the instances between results obtained by immunofluorescence and cultural methods. In addition, preliminary evidence indicated the feasibility of presumptive serotyping of Salmonella isolates by immunofluorescence.

Agglutination↗