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Biomedical subjects

C Rutherford

Publications and source records attributed to C Rutherford.

34 records · Page 2Linked to original sources
PubMed1989

The insulin-like growth factor II/mannose-6-phosphate receptor is present in fetal and maternal sheep serum.

A large mol wt binding protein for insulin-like growth factor II (IGF-II) has been described in fetal sheep serum. We now provide evidence to demonstrate that this binding protein is the IGF-II/mannose-6-phosphate (Man-6-P) receptor. Serum and plasma were gel filtered on Sephadex G-200, and the column fractions were assayed for binding of radiolabeled IGF-II. There was significant binding of [125I]IGF-II to the void volume fractions in addition to binding to the 150K and 40K carrier proteins. Binding to the void volume fractions was increased in fetal serum as well as maternal serum and dramatically decreased in the nonpregnant adult. Competitive binding studies with [125I]IGF-II and the void volume pools from fetal and maternal sheep serum demonstrated that IGF-I competed less potently than IGF-II, and insulin did not compete. There was no specific binding of [125I]IGF-I to the void volume pools of either fetal or maternal samples. Chemical cross-linking of [125I]IGF-II to aliquots of the void volume pools from fetal and maternal sheep serum samples and analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of dithiothreitol demonstrated a specific band at about 240K. Western blotting using a specific antiserum (no. 3637) against rat IGF-II/Man-6-P receptor was performed on aliquots of the Sephadex G-200 void volume pools of fetal, maternal, uterine vein, and adult sheep serum; a band of approximately 210K (without dithiothreitol) was seen. The IGF-II/Man-6-P receptor band was more intense in fetal serum than in either maternal or adult nonpregnant sheep serum. There was also increased binding of [125I]IGF-II in the 40K region of the Sephadex G-200 column fractions in the maternal serum compared to that in serum from nonpregnant adult ewes. When fetal, maternal, and adult nonpregnant sheep serum Sephadex G-200 pools were gel filtered on Sephadex G-50 in 1 mol/liter acetic acid to separate bound from free IGF, and IGF-II was measured by RRA, approximately 50% of the circulating IGF-II was associated with this IGF-II/Man-6-P receptor in fetal serum compared to 7% in maternal serum and 3% in adult nonpregnant serum. These data demonstrate that the IGF-II/Man-6-P receptor circulates in fetal sheep serum as well as maternal serum and appears to be a significant carrier for IGF-II in fetal sheep.

Animals↗
PubMed1978

An artifact produced by boiling adenyl cyclase reaction mixtures prior to the cyclic AMP-radioimmunoassay.

An artifact of procedure has been detected in the adaptation of the cAMP-radioimmunoassay to the analysis of adenyl cyclase activity. When reaction mixtures containing ATP and MgCl2 were boiled to terminate the enzyme assay, a cAMP-like decrease in antigen-binding was found. This decrease was dependent upon the presence of ATP, was linear to at least 5 min of boiling time, and could be destroyed with the addition of commercial phosphodiesterase. As significant levels of the nonenzymatically formed product resulted only when samples were boiled, alternative methods of terminating cyclase reactions are suggested.

Adenosine Triphosphate↗
PubMedDate not supplied

Immune thrombocytopenic purpura during pregnancy: laparoscopic treatment.

BACKGROUND AND OBJECTIVES: Laparoscopic surgical techniques in pregnancy have been accepted and pose minimal risks to the patient and fetus. We present the first reported case of a pregnant woman with immune thrombocytopenia purpura who underwent laparoscopic splenectomy during the second trimester. METHODS AND RESULTS: The anesthesia, hematology, and obstetrics services closely followed the patient's preoperative and intraoperative courses. After receiving immunization, stress close steroids, and prophylactic antibiotics, she underwent a successful laparoscopic splenectomy. After a short hospital stay, the patient was discharged home. CONCLUSION: Immune thrombocytopenia purpura can be an indication for splenectomy. As demonstrated in appendectomy, cholecystectomy, and our case presentation, laparoscopic splenectomy can be safely performed during pregnancy.

Adult↗
PubMedDate not supplied

Erythropoietin. A new frontier.

After more than 25 years of research and technology, a new product has recently been developed for the treatment of anemia associated with chronic renal failure in dialysis-dependent patients with end-stage renal disease. The availability of erythropoietin offers several advantages over other forms of treatment, such as red blood cell transfusions and androgens. This article describes the composition of erythropoietin, administration techniques, considerations for dosing, patient monitoring criteria, and patient education for those who receive it.

Anemia↗
PubMedDate not supplied

Fluid and electrolyte therapy: considerations for patient care.

In today's world of health care, the registered nurse who practices intravenous therapy must possess a thorough and up-to-date working knowledge of the intravenous fluids that are administered. This is not only necessary for safe nursing practice but is essential for excellent quality patient care. Due to the many types of intravenous fluids manufactured and administered today, it is extremely vital that the I.V. nurse be entirely familiar with each one before it is administered. This article will outline fluid, electrolyte and nutrient requirements as well as the most common I.V. fluids administered, their indications for use, complications and side effects.

Electrolytes↗
PubMedDate not supplied

Presidential address.

Explore the source record for details and available documents.

Home Care Services↗
PubMedDate not supplied

Intravenous administration of human immune globulin.

The intravenous infusion market is currently experiencing rapid growth. As a result, many new and varied therapies are being conducted in the hospital, outpatient, and home settings. One therapy that has been found to be safe and cost-effective to administer is I.V. IgG. This article will describe the contents of I.V. IgG as well as indications for use, product testing, dosage, effects, and adverse reactions.

Humans↗
PubMedDate not supplied

Taxol administration.

The majority of patients with ovarian cancer have widespread abdominal disease. Before 1970, the response rate for patients treated with chemotherapeutic agents was less than 10%. With the introduction of cisplatin, the response rate tripled. However, patients treated with repeated courses of platinum-containing regimens may develop resistance to this form of therapy. Taxol (Bristol Laboratories, Princeton, NJ), a new drug, has been shown to be an effective agent in patients who have failed first-line or subsequent chemotherapy for the treatment of metastatic carcinoma of the ovary even in women with platinum-resistant disease.

Drug Resistance↗