Biomedical subjects

C Russo

Publications and source records attributed to C Russo.

202 records · Page 12Linked to original sources

PubMedDate not supplied

Human Ia-like antigens (Analysis of the tissue distribution and immunochemical profile with monoclonal antibodies).

Analysis of the tissue distribution of human Ia-like antigens has shown that they have a wider distribution than originally reported. Furthermore, the expression of Ia-like antigens may change when cells undergo malignant transformation: for instance, melanoma cells acquire Ia-like antigens, while breast carcinoma cells lose them. Serological and immunochemical analysis of Ia-like antigens with monoclonal antibodies has shown a cellular and molecular heterogeneity of these molecules which had not been previously recognized with conventional allo- and xenoantisera. The functional significance of this heterogeneity is not known. Monoclonal antibodies to human Ia-like antigens cross-react with lymphocytes from other animal species indicating that portions of the molecules have been conserved during evolution. The biological implications of these findings are discussed.

Animals↗

PubMedDate not supplied

[Perioperative blood gas analysis in 100 patients in spontaneous respiration treated with ND-YAG laser for endoluminal tracheo-bronchial neoplasms].

OBJECTIVE: To determine the reliability on spontaneous ventilation of small continuously associated doses of ketamine and propofol during yag-laser therapy for upper airways neoplastic obstructions. DESIGN: Prospective description of blood gas analysis variation throughout the intervention and in the early postoperative period. SETTING: Operating theatre and postoperative Intensive Care Unit of the National Institute for Cancer Research. PATIENTS: A hundred consecutive cancer patients referred to our Institution for upper airways tumoral progressive obstruction. INTERVENTION: Yag-laser firing. MEASUREMENTS AND MAIN RESULTS: Pre-intra and postoperative blood gas analysis, BP, HR, Sat O2 have been registered every 10'. Mean intraoperative pCO2 rose to 47.3 mmHg (30-60), but within 2 to 3.20 hrs returned close to preoperative value (38.3 mmHg) allowing early patient discharge. CONCLUSIONS: The i.v. association of 0.7-1.0 mg/kg Ketamine and 1 mg/kg propofol, followed by 5 micrograms/kg/h and 3 mg/kg/h respectively, turned out to be satisfactory for both patients and anaesthetists in terms of anaesthesia and spontaneous breathing maintenance during yag-laser firing for obstructive upper airways cancer patients.

Adult↗

PubMedDate not supplied

[Association of the cytogenetic pattern and latero-cervical metastasis of laryngeal cancer].

Although solid tumors, in particular carcinomas, play a much larger part in human morbidity and mortality than hematological neoplasias do, much less is known about the cytogenetic abnormalities that characterize them. Numerous classification schemes have been used in the subdivision of human tumors. Most often the tumors are classified according to the presumed cell of origin (epithelial, mesenchymal, neurogenic or germ cell tumors), their benignity or malignancy, differentiation features and histological growth pattern or anatomical site or organ of origin. In the following discussion, we analyse 12 cases of squamous cell carcinoma of the larynx. All teh sample were karyotyped using a direct chromosome analysis method. The method reported was highly successful and in several types of tumors showed the possibility of obtaining good banded metaphases. The purpose of this research was to attempt to define the chromosomal pattern of laryngeal carcinomas and, consequently, to correlated, it to histological grading and clinical evolution. In all the cases studied we found several chromosomal anomalies. In five of them clonal anomalies, such as the loss of chromosome 4; Robertsonian translocation t(13,14); rearrangement of chromosome 1 and chromosome 17 and the presence of similar markers, were observed. The characterization of the markers was made with an IN SITU hybridization technique using specific probes, followed also in order to define two particular markers involving chromosome 1 and t(13,14). We did not reveal a correlation between histological grading and karyotype, but we did find a correlation between tumor ploidy and latero-cervical metastases. The most important result seems to correlate the presence of latero-cervical metastases with a hypodiploid cell line (with less than 46 chromosomes) in the primary tumor. This could be an index of the ability of the tumor to invade the latero-cervical lymph nodes. If this correlation is confirmed, conservative neck dissection could be limited to N0 patients with a hypodiploid pattern. In conclusion, we feel that, if confirmed, the results of this research, may be widely applied in clinical medicine.

Carcinoma, Squamous Cell↗

PubMedDate not supplied

[Cytogenetic patterns in Warthin tumor].

Our systematic studies of different types of benign and malignant salivary neoplasms in humans were initiated about two years ago. Analysis of karyotypes revealed that virtually all tumors are associated with chromosomal abnormalities. That those abnormalities may also take part in tumor initiation and progression is suggested by the association of specific chromosome rearrangement with particular cancers. We here with report cytogenetical observation in five Warthin's tumors giving particular attention to the possible occurrence of cells with (a) a normal karyotype or with (b) numerical changes, either loss of the Y chromosome or monosomy 4. The present series od adenolymphomas was karyotyped by a direct chromosomal analysis method. The method reported was highly successful, in several types of tumors showing the possibility of having good banded metaphases. All Warthin's tumors showed stemline (S) with a normal karyotype. These normal cells constituted the only S in two cases and the primary S in the other three cases. The normal cells observed in all adenolymphomas could be interpreted as outgrowing stroma cells. Most types of previously well-studied benign human tumor types, however (meningiomas, pleomorphic adenomas, lipomas), has a S group with a normal karyotype, regardless of the the tissue culture technique used. Thus, we believe that these normal cells usually represent neoplastic elements. Furthermore, we think that all benign tumors originate with a rather normal S, which, however, as the first indication of neoplastic transformation, shows chromosomal instability, such as the loss of chromosomes.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenolymphoma↗