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Biomedical subjects

C Roux

Publications and source records attributed to C Roux.

316 records · Page 18Linked to original sources
PubMedDate not supplied

Use of the acridine orange staining on smears of human spermatozoa after heat-treatment: evaluation of the chromatin condensation.

A simple, rapid procedure was described to evaluate the degree of chromatin compactness in human spermatozoa. Smears of fixed spermatozoa were heat-treated and stained with acridine orange. Smears from the same sperm samples were also stained with acidic aniline blue. Three groups of semen could be distinguished according to the percentage of red sperm heads observed under fluorescence microscope. 1) Semens with spermatozoa whose chromatin appeared normal before and after heat-treatment. 2) Semens with spermatozoa whose chromatin appeared normal before and abnormal after heat-treatment. 3) Semens with spermatozoa whose chromatin appeared abnormal before and after heat-treatment. The positive correlation between the percentages of red heads and the percentages of blue stained heads suggests that modifications in the biochemical composition of the basic protein component associated with DNA are responsible for the denaturation process.

Acridine Orange↗
PubMedDate not supplied

[Ultrasound scanning of abdominal contusions in children: experience at the Yopougon CHU (Ivory Coast)].

Ultrasound scans were used to study 23 cases of abdominal contusion in children, between January 1992 and December 1993. Thirteen boys and 10 girls were studied. They were all aged between 4 and 14 years, with a mean age of 6 years. The main causes of their injuries were road accidents (12 cases) and play (11 cases). Ultrasound scans were normal in 6 patients and pathological in 17 patients. The most frequently observed injuries were visceral effects (12 cases), almost always associated with hemoperitoneum. In three cases, hemoperitoneum was detected in the absence of visceral effects. The spleen (4 cases) and the liver (4 cases) were the most frequently injured organs. The other injuries detected included renal hematoma, bladder rupture and parietal hematoma. Diagnosis on the basis of ultrasound scans was found to be incorrect in three cases where diagnosis was repeated after the scan. One case involved a blocked perforation of the rectum, one a benign cyst and the other a mesenteric cyst. Despite these misdiagnoses, ultrasound scanning is a highly sensitive and specific method for examination of contusions in children. It is very useful and often sufficient for accurate diagnosis, particularly in units with only modest technical support.

Abdominal Injuries↗
PubMedDate not supplied

[Toxicology of reproduction: predictive value of experimental models. Round Table No 1 at Giens XIII].

The carrying out of clinical trials with a view to the marketing of drugs for human use is directly related to results of some animal studies. This workshop was devoted to evaluation of the quality and interest of these experimental models in reproductive toxicology. The predictive ability of preclinical trials to make extrapolations from animals to man decreases from foetotoxic to tetratogenic risks respectively and from the effects on fertility in both sexes to postnatal risks. As a result of this workshop, we propose the following improvements: (1) standardization and generalization of fertility test evaluations, especially the spermogram, in order to improve animal and human correlations; (2) development of knowledge and standardization of the follow up of the oestral cycle; (3) improvement of standardization, harmonization and diffusion of postnatal tests that prove relevant in animals; (4) increase in initiatives aimed at better mutual understanding of all drug partners; (5) creation of registers for new drugs, as soon as possible during clinical trials, to study their effects on the whole reproductive process; (6) recommendations for the creation of guidelines for International Conference on Harmonisation (ICH) to enable classification of observed effects in experimental models. This could lead to specific (potentially for each phase of the reproductive cycle) guidelines, precautions for use and/or contraindications which are listed in the summary of product characteristics.

Animals↗
PubMedDate not supplied

[Chromosomal study of 65 couples with spontaneous abortions].

A Robertsonian translocation and two reciprocal translocations were found in chromosome analysis of 65 couples attending for fetal wastage. The possible pathological significance of hyperdiploid cells which were observed is discussed.

Abortion, Spontaneous↗