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Biomedical subjects

C Rosse

Publications and source records attributed to C Rosse.

At least 73 records · Page 4Linked to original sources
PubMed1977

Hemopoietic repopulating potential of subcutaneous exudate cells.

Inflammatory exudate (SE) cells were collected from subcutaneous coverslips in mice and transferred into lethally irradiated (1,000 r) recipients. Eight days after transplantation 59Fe incorporation in the spleen and bone marrow was significantly greater than in controls treated with the suspending medium only. One hundred percent of mitoses were of the T6T6 karyotype in the marrow and spleen when SE cells were obtained from CBA/T6T6 donors. The repopulating potential of SE cells, however, lagged significantly behind that of bone marrow cells and the failure to observe consistently macroscopic spleen colonies calls into question whether the observed regeneration was due to pluripotent stem cells. Radioautographic studies with 3H-TdR showed that the majority of SE cells had recently been generated, but long-lived, noncycling cells of lymphoid and monocytoid morphology were also present in the exudate.

Animals↗
PubMed1977

Race-related differences in peripheral blood and in bone marrow cell populations of American black and American white infants.

The present study confirms that in healthy infants, a racial difference exists in peripheral blood hemoglobin levels, total leukocyte, and total neutrophil counts. Racial differences in the composition of the bone marrow are negligible. The only demonstrable difference is confined to the erythroid cell population. No significant difference exists in the incidence of myeloid cells or small lymphocytes.

Adult↗
PubMed1976

Cellular composition of the bone marrow in the chicken. I. Identification of cells.

Incorporation of Fe55 in vivo was used for verifying on radioautographs the identity of chicken bone marrow cells that are in the process of hemesyntheses. Under the experimental conditions all labeled cells may be considered to be linked with erythroid differentiation. They were classified into five maturational stages according to their morphology and capacity for DNA synthesis. Granulocytes were identified by the presence of specific granules. All mononuclear cells were classified as lymphocytes which had a pachychromatic nucleus, a high nucleocytoplasmic ratio, lacked the capacity for DNA synthesis, and resembled small lymphocytes of the bursa, spleen and bone marrow that bound, in vitro, anti-chicken gamma globulins labeled with I125. Radioautography with H3TdR was used to identify proliferating and non-proliferating members of each cell population.

Animals↗
PubMed1976

Cell proliferation in the erythroid compartment of guinea-pig bone marrow: studies with 3H-thymidine.

Single and multiple injections of 3H-TdR have been used for measuring the rate of proliferation in morphologically defined cell populations of guinea-pig bone marrow that are committed to erythroid differentiation. The conclusions are based on the analysis of absolute cell numbers in the maturational compartments, the labeling and mitotic indices, labeled mitotic curves, pulse and chase grain counts over dividing and interphase cells, and on the rate or labeling during multiple, repeated injections of 3H-TdR. The average duration of S and the rate of cycling is similar in all maturational compartments of the erythrom. The majority of cells progress to the next maturational compartment by the time they divide for the second time. All proerythroblasts and basophilic erythroblasts are in cycle. Polychromatic erythroblasts incapable of incorporating 3H-TdR reach the orthochromatic population in the span of 5-6 hr. The orthochromatic population is renewed every 20-24 hr. The number of divisions between the proerythroblast and orthochromatic erythroblast does not exceed four and some cells may undergo only two divisions during the maturation pathway. Cell input from a progenitor cell population contributes to the maintenance of the erythron. The kinetic behavior of progenitor cells is similar to that of proerythroblasts. By the time of their second division, progenitor cells may reach either the proerythroblast or basophilic erythroblast compartments. The kinetic behavior of basophilic transitional cells corresponds to the predicted behavior of the erythroblast progenitor cell pool. Several of the conclusions are based on the assumption that grain count halving is the result of cell division. In view of the evidence discussed, this assumption in the present studies seems justified.

Animals↗
PubMed1975

Voluntary election of basic science courses by medical students.

The medical curriculum at the University of Washington places considerable responsibility on the student in planning his education. This is provided for by assigning to elective courses 50 percent of the credits required for graduation. While the student has considerable freedom to choose among electives, as many credits are elected in the basic sciences as in the clinical disciplines. Four consecutive entering classes elected a disproportionately high number of credits in anatomy and pharmacology. A need for basic science courses in apparently perceived by the medical student, and their voluntary election is bound to facilitate learning. In new curricula the responsibility rests on the faculty to provide opportunities for detailed coverage of the various disciplines in order to cater to those students who are motivated to pursue them in greater depth.

Anatomy↗
PubMed1975

Comparison of lymphocyte populations bearing surface immunoglobulins in avian bone marrow, bursa, spleen and thymus.

Rabbit anti-chicken gamma-globulin was labeled with 125I and then incubated with cells from the bursa, thymus, spleen, and bone marrow of 4- and 8-week old birds. The same procedure was carried out on 11-week-old agammaglobulinemic chickens. Autoradiography revealed that the majority of large, medium, and small bursal lymphocytes bind the antibodies while labeled lymphocytes of each type in the spleen and thymus never exceeded 11 or 4 percent, respectively. Labeled medium and small lymphocytes in the bone marrow increased from 4.2 and 1.7%, respectively, at 4 weeks of age, to 9.5 and 8.8%, respectively, at 8 weeks of age. Labeled lymphocytes of all sizes were completely absent in all tissues of agammaglobulinemic chicks, including the marrow. Therefore, the increase in frequency of labeled lymphocytes in the bone marrow with age may be a result of recruitment of cells from the bursa of Fabricius. The majority of lymphocytes in the bone marrow do not label. Therefore, lymphocytes from the bone marrow may be T cells, subsets of B cells, or neither T or B cells.

Agammaglobulinemia↗