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Biomedical subjects

C Richard

Publications and source records attributed to C Richard.

At least 307 records · Page 17Linked to original sources
PubMed1985

[In France, is acute alcoholic intoxication in adults a metabolic emergency?].

Some biological data of 63 acute alcoholic poisoning (without drug ingestion) in adults were studied during a brief hospitalization. Mean arterial blood concentration of lactate was 2,34 mmol/l +/- 0,13, of ethanol was 53 +/- 3,68 mmol/l. No correlation was found between these two variables and we have not registered lactic acidosis. Arterial blood acid base balance was normal for 24 of 38 unconscious patients. The results of other fourteen showed moderate acidosis, either mixed or respiratory either metabolic, without hypoglycemia or ketonuria. No correlation between arterial blood ions H+ concentration (44,72 +/- 1,18 nmol/l (m +/- sem) and concomitant arterial blood ethanol concentration was found. Moreover, no correlation between 46 blood glucose concentrations (without prior glucose perfusion) and alcoholemia was found. Only two 18 hours fasting chronic alcoholics showed hypoglycemia. These results confirm that acute ethanol intoxication is a potential respiratory emergency in adults, and not a metabolic one (this last pattern being true for children). No confusion between acute intoxication and alcoholic ketoacidosis, alcohol induced fasting hypoglycemia or shoshin beriberi must be made.

Adolescent↗
PubMed1985

[Diagnostic and therapeutic use of human anti-D (Rho) monoclonal antibodies. Evaluation and perspectives].

Human monoclonal antibodies will be essential in medicine. They are valuable tools for biological diagnosis and therapeutics. Our model, human monoclonal antibodies directed against the Rhesus D antigen can be used for the determination of the Rhesus D phenotype and for the suppression of Rh(D) immunisation in women. These new products require new procedures of preparation, new regulations for the quality controls, which will be discussed in this paper.

Animals↗
PubMed1985

[Lack of specificity of protected distal bronchial samples in the bacteriological diagnosis of nosocomial pneumonia caused by gram negative bacilli].

Lower respiratory tract secretions (LRS) were sampled during a two year period in patients undergoing mechanical ventilation using a method previously described by Matthew et al. Only those LRS positive for gram-negative bacilli were reported in this study. The patients were separated in two groups: those with nosocomial pneumonia due to gram-negative bacilli (NP+ group) and those without (NP- group). The species most often isolated from LRS were: Pseudomonas aeruginosa (25%), Proteus (20%), Escherichia coli (18%), Klebsiella pneumoniae (10%), Acinetobacter calcoaceticus (8%) and Haemophilus influenzae (7%). The spectrum of gram-negative species was not statistically different in either group of patients. The numbers of gram-negative bacilli isolated from each LRS were more important in the NP+ group (p less than 0.001), but so was the duration of mechanical ventilation before each LRS (p less than 0.01). In this group also, a specific bacteriological diagnosis with only one species was possible in 3 out of the 13 cases; a tentative diagnosis only was possible in 10 cases: in 6 cases, the responsibility of two (or more) species was suggested. The susceptibility to antibiotics of gram-negative bacilli isolated from LRS was moderate. A third generation cephalosporin together with amikacin seemed to be the best choice of antibiotics when no specific bacteriological diagnosis could be made with the LRS.

Anti-Bacterial Agents↗
PubMed1985

[Right endocarditis in disseminated aspergillosis].

A 53 year old man with an anaplastic bronchial carcinoma was hospitalised for septic shock and acute respiratory distress after a cutaneous, probably staphylococcal infection, and died in spite of anti-staphylococcal antibiotherapy. The autopsy showed pulmonary, cardiac, cerebral and renal aspergillosis. A right heart aspergillous endocarditis, very rare in this pathology, was also discovered but there were no cardiac valves lesions. The patient was in an "immunodepressed" state as usually observed in pulmonary aspergillosis. The endocardial localisation of aspergillosis and the "pseudo-miliary" appearances of the pulmonary lesion indicated an extra-pulmonary portal of entry, cutaneous or intravenous which is unusual in this pathology. This hypothesis is supported by previous reports of pulmonary aspergillosis where right heart endocarditis is exceptionally rare and by aspergillous left heart endocarditis after open heart surgery where pulmonary aspergillosis is absent.

Aspergillosis↗
PubMed1985

[Antibiotic resistance of Escherichia coli. Importance of the resistance to trimethoprim-sulfamethoxazole].

Antimicrobial sensitivities, especially trimethoprim-sulfamethoxazole, were studied in all clinical isolates of Escherichia coli in an intensive care unit for over 18 months. Twenty-four per cent of strains were resistant to trimethoprim-sulfamethoxazole. Combined resistance to ampicillin +/- chloramphenicol (+/- tetracycline) and streptomycin (+/- kanamycin) with resistance to trimethoprim-sulfamethoxazole was demonstrated. These data confirm the previously reported increasing trimethoprim-sulfamethoxazole resistance which is probably plasmid-mediated and specify the resistances associated with trimethoprim-sulfamethoxazole resistance. These findings suggest that widespread prophylaxis in granulocytopenic patients with lower urinary tract infection by the trimethoprim-sulfamethoxazole association should be re-examined.

Anti-Bacterial Agents↗
PubMed1985

Influence of acetylsalicylic acid on bleeding time and serum thromboxane B2 in diabetes mellitus type I.

Basal and two hours after 600 mg acetylsalicylic acid (ASA) bleeding times were measured in 21 type I diabetic patients with retinopathy, 24 type I diabetic patients without retinopathy and 21 normal healthy volunteers. There were no significant differences either in basal or in after ASA bleeding times between these groups, but the percentage increase in bleeding time after ASA was significantly higher than normal in both diabetic groups. No correlation was found between basal--bleeding time and glucose, Hb A1 or lipid profile. TXB2 production by spontaneous blood clotting was drastically reduced by ASA in both diabetic and normal groups. Platelet hyperactivity in diabetes mellitus may be due, at least in part, to a predominance of the proaggregatory effects of TXA2 over the antiaggregatory effects of PGI2.

Adolescent↗
PubMed1985

[Characteristics of phenotypes of Alteromonas putrefaciens. Study of 123 strains].

The phenotypic characteristics of 124 strains of Alteromonas putrefaciens, a bacterium that produces large quantities of hydrogen sulfide, were studied. The strains were isolated from man, warm and cold-blooded animals (especially fish and shellfish), marine and fresh waters. Two phenetic groups were differentiated: group A strains grew at 42 degrees C, but not at 4 degrees C, tolerated 6 p. cent NaCl and did not utilize glucose or maltose, unlike group B strains. Each group could be divided into two subgroups A1, A2 and B1, B2. Citrate utilization, hydrolysis of urea and aesculin, resistance or susceptibility to cephalothin and vibriostatic agent 0/129 were useful for subgroup determination. The taxonomic relatedness of A. putrefaciens to genus Alteromonas are discussed.

Animals↗
PubMed1984

Acquired von Willebrand's syndrome associated with hydatid disease of the spleen--disappearance after splenectomy.

A new case of acquired von Willebrand's syndrome (vWS) is described in a 31-year-old woman with a hydatid splenomegaly and with a history of repeated abortions at an advanced stage of pregnancy, a positive serology for syphilis and a mildly elevated titre of antinuclear antibodies, with no family history of bleeding. There is an inhibitory effect on factor VIII: C (antihaemophilic factor) as well as on factor VIIIR: Ag (related antigen) and on factor VIIIR: RCo (ristocetin cofactor), and it is precipitated by rabbit anti-IgG antiserum. This inhibitory effect was demonstrated using the patient's plasma heated to 56 degrees C for one hour so as to dissociate circulating immunocomplexes. All the abnormalities of haemostasis, as well as the positive serology for syphilis, disappeared after splenectomy, and the ANA titre reverted to normal. The clinical and biological peculiarities of the case are discussed, and are interpreted in the light of the findings recorded in patients showing "lupus" anticoagulant.

Adult↗
PubMed1984

[Acute immunoallergic interstitial nephritis due to noramidopyrine].

A 46-year old patient under noramidopyrine treatment was admitted for acute renal failure. Renal biopsy was performed and showed acute interstitial nephritis without staining at immunofluorescent microscopy. In vitro and in vivo immunological tests using noramidopyrine as antigen were all negative. Abnormal serum creatinine levels were still present 4 months later. To our knowledge, noramidopyrine has rarely been the cause of interstitial nephritis (only one other case has been published), but it has frequently been responsible for drug-induced blood diseases, and noramidopyrine-related drugs have previously been involved in drug-induced nephritis. As the renal damage can probably be explained by a delayed hypersensitivity reaction, an early corticosteroid treatment would help in improving renal function.

Acute Disease↗
PubMed1984

Comparison of the tryptic digestion pattern of subfragments 1 from V1 and V3 rat cardiac isomyosins.

The limited tryptic digestion patterns of the chymotryptic subfragment 1 (S1) of the two rat ventricular isomyosins V1 and V3, were compared under several conditions. Pure S1V1 was obtained from 3-week-old rats and pure S1V3 from adult rats 6 weeks after hypophysectomy. To localize the sites of trypsin susceptibility and to determine the distribution of the peptides along the S1 molecule, we used, as a probe, antibodies raised against a pig cardiac 29-kDa peptide. We demonstrate that this peptide contains the N-acetyl group located on the N-terminal part of the cardiac myosin molecule. In S1V1 we observed two major sites of proteolysis, independently of the digestion conditions: they are located at 27kDa and 80kDa from the N terminus as in skeletal muscle S1.S1V3 appears much more sensitive to the proteolysis conditions: at least two additional sites of cleavage are present in the 50-kDa peptide when digested at pH 8.0. Decrease in the pH from 8.0 to 7.0 or the presence of Mg-ATP have no effect on the digestion of S1V1 while these ambient factors protect the 50-kDa peptide of S1V3 from breakdown. We conclude that the 50-kDa peptide is a variable portion of the myosin molecule, the conformation of which is sensitive to ambient factors such as the pH or the presence of nucleotides.

Amino Acids↗
PubMed1984

Special problems encountered during the cyanogen bromide cleavage of lobster arginine kinase.

During structural analysis of Lobster muscle arginine-kinase, we have isolated a CNBr resulting peptide with a blocked N-terminal residue. This peptide was sequenced after unblocking by mild acid treatment (1 N HCl at 100 degrees C for 10 min). The blocked form is not due to the formation of pyroglutamic acid nor is it due to the formation of diketopiperazine. We have applied the experimental conditions used for CNBr cleavage of lobster arginine-kinase to a synthetic peptide the structure of which is similar to the above CNBr peptide. We bring evidence that during CNBr cleavage partial formylation occurs with a possible cyclization of a 7 membered ring of Gly--Asp...

Amino Acid Sequence↗