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Biomedical subjects

C Rampini

Publications and source records attributed to C Rampini.

At least 37 records · Page 2Linked to original sources
PubMed1978

[Characterization of the partial inhibition, by deficiency in serum factors, of multiplication of SV3T3 transformed fibroblasts].

Partial inhibition, by serum starvation, of multiplication of SV3T3 transformed fibroblasts was studied by cultivating cells in a medium containing 1% of serum. Two different and successive states are described: a first step, with a rate of multiplication for 24 hrs, which is lowered (2 instead of 3 in a normal medium containing 10% of serum) but which remains constant (i.e. exponential multiplication), the observed inhibition being reversed by normal medium; a second step, where the rate of multiplication for 24 hrs, decreases very fast, and which is not reversible.

Blood↗
PubMed1977

[Sensitivity of transformed EHSVi fibroblasts to the antibody-complement lytic system. Cooperation between the lytic units, and existence of a cellular defense].

The sensitivity of transformed EHSVi fibroblasts to the antibody-complement lytic system was evaluated, at various antibody concentrations (Ac), in conditions in which a variation of the lytic activity proportional to Ac3, 1 may be observed. This points out, not the expected cooperation between IgG molecules in activating C1 within a definite lytic unit, but a cooperation between the lytic units themselves, and therefore the existence of a cell defence.

Antibodies↗
PubMed1975

[Hydrolysis of diphosphatidylglycerol in situ in E. coli by activation of specific phospholipase D].

E coli cells are harvested at early stationary phase and, first, incubated in phosphate buffer without energetic metabolites, conditions which invole the formation of an excess of diphosphatidylglycerol. If, in a second stage, they are cultivated again in normal medium, the diphosphatidylglycerol is metabolized very fast. It is mainly hydrolyzed, by activation of a specific phospholipase D, with formation of phosphatidylglycerol and phosphatidic acid. The latter is immediatly metabolized in bacteria in phosphatidylglycerol and phosphatidylethanolamine, both about equal in amount.

Culture Media↗
PubMed1975

[Phospholipid composition and turnover in normal and SV40 transformed fibroblasts. Effect of cell density].

The phospholipid composition and turnover in normal and in SV 40 transformed hamster fibroblasts were studied. The amount of phospholipid phosphorus relative to protein is lower in transformed hamster fibroblasts than in normal fibroblasts. This amount decreases with increasing cell density until stationary growth is reached. The decrease is largest for the normal fibroblasts. In transformed cells, less sphingomyelin and more diphosphatidyl glycerol are found than in normal cells. The turnover of 32P in sphingomyelin is slower in transformed cells than in normal cells ; the contrary is observed with diphosphatidyl glycerol. On the other hand, in transformed cells, phosphatidyl ethanolamine has a faster turnover than phosphatidyl choline, whereas the contrary is observed in normal cells. Finally, the change to stationary growth slows down the turnover of 32P of all phospholipids, this decrease being more important in transformed cells.

Cell Count↗
PubMedDate not supplied

Phosphate dilution lowers net phosphatidyl choline synthesis in lymphocytes.

The rate of net phosphatidyl choline (PC) synthesis in lymphocytes was evaluated, under appropriate conditions, in terms of [32P]phosphate incorporation. Phosphate-diluted and normal media were compared, with the cells being stimulated by concanavalin A or not. In either case, incubation in phosphate-diluted medium lowered net PC synthesis. In concanavalin A-stimulated cells, phosphate dilution also abolished the stimulation effect on net PC synthesis in G1. Nevertheless, these changes did not inhibit blastogenic transformation of cells.

Cells, Cultured↗
PubMedDate not supplied

[Isolated muscular sarcoidosis mimicking a tumoral lesion].

We report a case of a woman with a palpable painful nodule on her left leg. MR and CT showed a lesion that could be described as a neoplasm. Excisonal biopsy revealed a noncaseating granuloma. The woman presented the nodular type of muscular isolated sarcoidosis. Further the disease involved the lungs; this confirmed the accurate diagnosis of sarcoidosis. Sarcoidosis is a chronic, multisystem granulomatous disease of unknown etiology. Muscle involvement is frequent, but often asymptomatic. There are three forms of muscular sarcoidosis: only the nodular type can be recognized by technical imaging. MR and ultrasound are the best methods to attempt the diagnosis of nodular muscular sarcoidosis; nevertheless, the lesion must have a standardized behaviour because it can mimic a malignant neoform. In this case, biopsy is the only tool to identify the disease.

Diagnosis, Differential↗