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Biomedical subjects

C R Walker

Publications and source records attributed to C R Walker.

At least 19 recordsLinked to original sources
PubMed2001

Movement of the knee in osteoarthritis. The use of electrogoniometry to assess function.

We used electrogoniometers to measure the range of movement (ROM) of the knee during various activities, comparing 50 patients with osteoarthritis of the knee (OA) with 20 healthy age- and sex-matched subjects. The minimum and maximum joint angles and the ranges of excursion of the patient and control groups were tested for significant differences, using an unrelated Student's t-test with pooled variance. Knee flexion in patients with OA was significantly reduced during all activities (p < 0.05), but differences in knee extension were not significant except when patients negotiated stairs. We believe that this reduction in ROM is caused by inhibition due to pain when load-bearing. Static non-load-bearing measurements of the ROM poorly reflected the functional ROM, with a coefficient of determination (r2) of 0.59 in the patient group and 0.60 in the control group. Electrogoniometry of the ROM of the knee provides a reliable, accurate and objective measurement of knee function.

Aged↗
PubMed2001

Influence of missing values on artificial neural network performance.

The problem of databases containing missing values is a common one in the medical environment. Researchers must find a way to incorporate the incomplete data into the data set to use those cases in their experiments. Artificial neural networks (ANNs) cannot interpret missing values, and when a database is highly skewed, ANNs have difficulty identifying the factors leading to a rare outcome. This study investigates the impact on ANN performance when predicting neonatal mortality of increasing the number of cases with missing values in the data sets. Although previous work using the Canadian Neonatal Intensive Care Unit (NICU) Network s database showed that the ANN could not correctly classify any patients who died when the missing values were replaced with normal or mean values, this problem did not arise as expected in this study. Instead, the ANN consistently performed better than the constant predictor (which classifies all cases as belonging to the outcome with the highest training set a priori probability) with a 0.6-1.3% improvement over the constant predictor. The sensitivity of the models ranged from 14.5-20.3% and the specificity ranged from 99.2- 99.7%. These results indicate that nearly 1 in 5 babies who will eventually die are correctly classified by the ANN, and very few babies were incorrectly identified as patients who will die. These findings are important for patient care, counselling of parents and resource allocation.

Decision Support Systems, Clinical↗
PubMed2000

Escherichia coli alpha-hemolysin (HlyA) is heterogeneously acylated in vivo with 14-, 15-, and 17-carbon fatty acids.

alpha-Hemolysin (HlyA) is a secreted protein virulence factor observed in certain uropathogenic strains of Escherichia coli. The active, mature form of HlyA is produced by posttranslational modification of the protoxin that is mediated by acyl carrier protein and an acyltransferase, HlyC. We have now shown using mass spectrometry that these modifications, when observed in protein isolated in vivo, consist of acylation at the epsilon-amino groups of two internal lysine residues, at positions 564 and 690, with saturated 14- (68%), 15- (26%), and 17- (6%) carbon amide-linked side chains. Thus, HlyA activated in vivo consists of a heterogeneous family of up to nine different covalent structures, and the substrate specificity of the HlyC acyltransferase appears to differ from that of the closely related CyaC acyltransferase expressed by Bordetella pertussis.

Acylation↗
PubMed1999

Extension of the elbow and supracondylar fractures in children.

We tested the hypothesis that children who sustain a supracondylar fracture have a greater range of elbow hyperextension than those with a fracture of the distal radius. Three observers made 358 measurements in 183 children (114 boys and 69 girls). There were 119 fractures of the distal radius and 64 supracondylar fractures. Initially, the group with a supracondylar fracture appeared to have extension 1.7 degrees greater than that of the group with fracture of the distal radius. On average, there was a maximum variation of 3 degrees between observers. After allowing for age, gender and observer, there was no significant difference between the groups. Our study had greater than 80% power to detect a difference in hyperextension of 2 degrees at the 5% level with the above observer variability. When age and gender are taken into account, any variation in the amount of hyperextension at the elbow is not sufficient to explain the occurrence of a supracondylar fracture.

Adolescent↗
PubMed1999

Intraoperative bacterial contamination in operations for joint replacement.

All surgical operations have the potential for contamination, and the equipment used can harbour bacteria. We collected samples from 100 elective primary hip and knee arthroplasties. These showed rates of contamination of 11.4% for the sucker tips, 14.5% for light handles, 9.4% for skin blades and 3.2% for the inside blades used during surgery; 28.7% of gloves used for preparation were also contaminated. Of the samples taken from the collection bags used during hip arthroplasty, 20% grew bacteria, which represents a significant microbial reservoir. Also, 17% of theatre gowns were contaminated at the end of the operation. Contamination was found in 10% of the needles used during closure of the fascia. Overall, 76% of the organisms grown were coagulase-negative staphylococcus. A total of 63% of operations showed contamination in the field of operation. Some changes in practice are suggested. Follow-up for a minimum of two years revealed one deep infection but the organism was not identified as a contaminant. These data provide a baseline for studying the bacteriology of the surgery of revision arthroplasty.

Arthroplasty, Replacement, Hip↗
PubMed1996

Interleukin-6 expression in cord blood of patients with clinical chorioamnionitis.

The objective of this study was to define whether IL-6 is an early marker of infection in the newborn. To correlate the occurrence of clinical chorioamnionitis with the levels of IL-6 expression in neonates, IL-6 was measured in cord plasma by ELISA and in mononuclear cells by reverse transcriptase-PCR before and after mitogenic stimulation. Eight neonates were included in each of the following four groups: elective cesarean section, uncomplicated normal spontaneous vaginal delivery, delivery after prolonged rupture of amniotic membranes with no evidence of chorioamnionitis, and delivery with evidence of chorioamnionitis. All 32 neonates were clinically well after delivery, and all 16 babies with prolonged rupture of membranes or clinical chorioamnionitis had negative blood cultures. Elevated IL-6 levels were found only in neonates born to mothers with chorioamnionitis (119.7 +/- 33.5 pg/mL versus 2.71 +/- 0.59 pg/mL, p < 0.005). Mononuclear cells from five of these neonates expressed no IL-6 mRNA in vivo despite elevated levels of IL-6 in their cord plasma. Cord blood mononuclear cells from healthy term babies were capable of synthesizing IL-6 in vitro in response to stimulation with bacterial lipopolysaccharide. These results suggest that IL-6 levels in cord plasma increased with clinical chorioamnionitis, despite the lack of evidence of infection in the neonates. Therefore, we conclude that, although a high level of IL-6 may be a good marker of chorioamnionitis, it may not be a specific marker of infection in the newborn.

Chorioamnionitis↗
PubMed1994

Lower limb amputation following injury: a survey of long-term functional outcome.

Although there have been significant advances in surgical methods of limb preservation and various injury scores produced to predict the outcome after severe lower limb injury, there is common belief amongst surgeons that patients rehabilitate rapidly and well after amputation. There is however little evidence in the literature supporting this view of functional outcome of amputation following injury. A subjective assessment of post-injury lower limb amputees implied that there was a similar functional outcome in above- and below-knee amputations. Furthermore, there was little functional difference between early and delayed amputations, the delayed group being more satisfied with the end result.

Adolescent↗
PubMed1994

Case report: complete photopenia of the femoral head on radionuclide bone scanning in septic arthritis of the hip.

Three cases of septic arthritis of the hip are presented. In each case, radionuclide bone scanning, using 99Tcm diphosphonate (MDP), demonstrated complete photopenia of the femoral head. All three cases resulted in avascular necrosis and subsequently had a poor clinical outcome with a reduced range of movement and radiographic epiphyseal deformity. Complete photopenia in the presence of suspected sepsis requires urgent referral with a view to exploration and decompression.

Arthritis, Infectious↗
PubMed1993

Five to eight year results of the Johnson-Elloy (Accord) total knee arthroplasty.

Between February 1982 and December 1985, 133 knees in 107 patients were replaced with a cemented Johnson-Elloy (Accord) total knee arthroplasty. Thirty-five knees were lost during the follow-up period because of death in 29, revision in 3, infection in 1, and refusal of follow-up evaluation in 2. The results of the remaining 98 knees in 76 patients with a 5-8 year follow-up period are presented. The procedure was carried out in all cases presenting for surgery, irrespective of pathology and degree of deformity. The range of flexion achieved as a mean of 93.5 degrees in the osteoarthritic group and 100 degrees in the rheumatoid arthritic group. Eighty-seven percent of the osteoarthritic group and 95% of the rheumatoid arthritic group achieved between 10 degrees and 50 degrees of rotation at 90 degrees of flexion, which was maintained for the duration of the study. Adequate stability in both groups, valgus-varus and rotation in extension, and anteroposterior in flexion was achieved. Survivorship was 97.7% at 80 months.

Aged↗
PubMed1992

Dexamethasone therapy for bronchopulmonary dysplasia: improved respiratory mechanics without adrenal suppression.

The purpose of the present study was to examine the pattern of changes in respiratory system mechanics induced by dexamethasone (Dex) in infants with bronchopulmonary dysplasia (BPD) and to determine whether dosages that produce these changes induce adrenal suppression. We examined mechanics in seven ventilator-dependent premature infants (age, 33 +/- 4.8 days) with BPD, before and daily during Dex therapy. Dex (0.5 mg/kg/day) was given intravenously for 7 days unless complications necessitated early termination. Respiratory system resistance (Rrs) and compliance (Crs) were measured by the passive expiratory flow-volume technique during the course of dexamethasone therapy or until extubation. Adrenocorticotrophic hormone (ACTH) stimulation tests were done at baseline and following Dex therapy to evaluate adrenal function. Dex therapy caused a 77 +/- 18% increase in Crs (from 0.97 +/- 0.09 SEM mL/cmH2O to 1.6 +/- 0.16 mL/cmH2O; P less than 0.025) and a 33 +/- 5% decrease in Rrs (from 0.20 +/- 0.02 cmH2O/mL/s to 0.14 +/- 0.01 cmH2O/mL/s; P less than 0.01). Concurrently, ventilator rate, mean airway pressure, and FIO2 all decreased significantly (P less than 0.025). Extubation occurred later in infants with the lowest Crs and highest Rrs at baseline. At extubation, all Crs values were greater than 1.33 mL/cmH2O and Rrs values were less than 0.15 cmH2O/mL/s. Systolic blood pressure increased from 61 +/- 6.3 mmHg to 84 +/- 17 mmHg, 72-96 h after the start of Dex (P less than 0.025). There were no episodes of culture-positive sepsis. Neither basal nor ACTH-stimulated levels of cortisol were suppressed as a result of Dex therapy (P greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Glands↗
PubMed1991

Colon cancer in blacks: a disease with a worsening prognosis.

Despite the fact that a significant national effort has been made over the past 10 years to improve the early detection and treatment of colorectal cancer, blacks have not had any change in their prognosis compared to whites, and their survival rate appears to be independent of stage at the time of diagnosis. The disease incidence is practically the same for both races, 49 per 100,000. We reviewed all patients with colorectal cancer in our Tumor Registry over a 10-year period to determine whether the experience at an all-black institution with a black patient base for much of its history would help clarify these crucial questions. There were 118 cases (73 women/45 men), and the mean age was 68 years (range: 29 to 93). The most common signs and symptoms were gross bleeding (34%) and abdominal pain (30%), with most patients presenting with a combination of symptoms. Remarkably, none were symptom-free. Of the 96 patients who were staged surgically and pathologically, 68 (71%) were beyond Duke's B staging at the time of diagnosis and surgery. The overall 5-year survival rate was 47%, significantly related to stage of disease at diagnosis (P less than .001). We concluded that blacks have not shared in the progress made in early diagnosis and treatment of colon cancer, and that special attention should be given to developing screening and surveillance methodology targeted specifically at blacks.

Adenocarcinoma↗
PubMed1981

Regulation of myosin accumulation by muscle activity in cell culture.

Tetrodotoxin (TTX), at concentrations that do not interfere with normal myogenesis or with myosin synthesis, causes of cultured muscle fibres to accumulate myosin heavy chain peptides. This effect is now shown to be reversible. On removal of TTX, muscle fibres begin to reaccumulate myosin heavy chains and it appears that the myosin heavy chains display a 230% increase in stability when cells are shifted from TTX to a normal medium without TTX. Total protein stability or turnover is not affected by TTX. The ability of TTX to induce failure of accumulation of myosin heavy-chain in cultured muscle fibres does not extend to cultured chick fibroblasts. TTX also does not perturb normal uptake of [3H] leucine during a 1 h pulse and the leucine-specific activity within TTX-treated cells is essentially equivalent to that within normal cells. Finally, limited proteolysis of myosin heavy chain isolated from TTX-treated and normal muscle fibres and display of cleavage products on SDS-polyacrylamide gels does not reveal any significant difference between the two myosins. We conclude that failure of TTX muscle to accumulate myosin heavy chain is not related to impaired synthesis, to changes in myosin heavy-chain primary structure, or to overall changes in muscle fibre proteolytic activity. We speculate that the increase in degradation and resulting failure to accumulate myosin heavy chain in TTX cells is related to an inability of TTX-related muscle fibres to assemble newly synthesized fibrillar proteins into structures such as filaments or fibrils. Failure of assembly would lead to increased exposure to base-line levels of muscle proteolysis and to the observed lack of accumulation of myosin heavy chain.

Animals↗
PubMed1981

Diazepam binding of dissociated hippocampal cultures from fetal mice.

Primary cultures of dissociated hippocampi from fetal mice examined for the presence of binding sites for [3H]diazepam. The binding assays were done with living cells still attached to the culture dish. The cells contain high affinity binding sites for [3H]diazepam, Kd = 5 nM, which are completely inhibited with 20 nM R05-4864 but only 26% with 20 nM lorazepam. The binding was inhibited by purinergic compounds and by quinidine. The living cell did not exhibit increased binding of [3H]diazepam in the presence of GABA and in fact a slight decrease in binding was found. This was also found when live, intact C6 glial cells were investigated. These observations suggest that the use of living cells to study the benzodiazepine receptor is valuable and maybe necessary to fully characterize this receptor.

Animals↗
PubMed1981

Development of GABAergic function of dissociated hippocampal cultures from fetal mice.

The GABA uptake mechanism has been characterized in hippocampal cell cultures prepared from fetal mice of 13-19 days gestational age [3H]GABA is accumulated selectively by neuronal cells by a high-affinity (Km = 3 micro M) mechanism that is an early property of the neurons. Autoradiography of [3H]GABA uptake revealed that approximately 30% of the neuronal cells had uptake. A unique small neuronal type did not have [3H]GABA uptake but was found to have [3H]glutamic acid uptake, suggesting that these neurons may be granule cells. The selective labeling of neurons with [3H]GABA and [3H]glutamic acid is consistent with the idea that high-affinity uptake of a transmitter may be unique to neurons that use that transmitter.

Animals↗