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Biomedical subjects

C R Lowe

Publications and source records attributed to C R Lowe.

At least 127 records · Page 7Linked to original sources
PubMed1973

Some parameters relevant to affinity chromatography on immobilized nucleotides.

1. The suitability of cellulose and Sepharose as supports for affinity chromatography of two groups of cofactor-linked enzymes, dehydrogenases and kinases, was examined. Sepharose was found to be superior. 2. The selective capacities of the columns were measured by frontal analysis and are discussed in relation to the nucleotide contents. 3. The effect of various concentrations of enzyme and of non-specific protein on the performance of the affinity columns, and the effects of equilibration time, flow rate, sample volume and dilution of the nucleotide were examined. 4. The effect of interposing polymethylene and polyglycine extension arms between the matrix backbone and the nucleotide was investigated for several cofactor-dependent enzymes. Maximum binding was observed with an extension arm 0.8-1nm long.

Adenosine Triphosphate↗
PubMed1973

The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.

1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and other interacting ligands can be made to selectively alter the chromatographic behaviour of the desired enzyme.

Adenosine Diphosphate↗
PubMed1973

Affinity chromatography of lactate dehydrogenase on immobilized nucleotides.

The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immobilized NAD(+) was investigated. The interaction of both lactate dehydrogenase isoenzymes with immobilized pyridine and adenine nucleotides and their derivatives were measured. The effects of these parameters on the interaction of lactate dehydrogenase with immobilized nucleotides were correlated with the known kinetic and molecular properties of the enzymes in free solution.

Adenine Nucleotides↗
PubMed1972

Congenital malformations and the problem of their control.

The possibility of preventing or reducing the prevalence of human congenital malformations is discussed in the light of current knowledge about their aetiology. The original data presented are derived from an epidemiological study of all the infants (92,982) born in the three years 1964-6 to women resident in a defined area of South Wales and all the congenital malformations (3,242) discovered in those infants by the second anniversary of their birth.

Abnormalities, Drug-Induced↗
PubMed1971

Obesity and smoking habits.

A large-scale survey of steel workers in South Wales has shown a considerable difference between the body weights of smokers and of non-smokers. The difference increases with age so that men over 40 years who have never smoked are on average 13 lb (5.9 kg) heavier than smokers. Even so, smokers are about 15 lb (6.8 kg) heavier than the weight standard considered desirable by the Metropolitan Life Insurance Company, while non-smokers are nearly 30 lb (13.6 kg) heavier.About 20% of the men are attempting to give up the smoking habit. Ex-smokers who have given up smoking for more than eight years approach the body weight of men of the same age who have never smoked.Many reports have been published on the health consequences of smoking and of obesity. Because smoking and obesity are inversely related studies of the interrelation of these two health hazards and of their relative importance are needed.

Adult↗
PubMed1971

Malformations of central nervous system and softness of local water supplies.

The mean annual prevalence at birth of congenital malformations of the central nervous system for the three years 1964-6 in 48 local authority areas in South Wales was negatively correlated (r = -0.402, P<0.01) with estimates of the mean total hardness of the related water supplies.The mean annual perinatal mortality rate from anencephalus for the five years 1963-7 in 58 county boroughs in England and Wales was also negatively correlated with estimates of the mean total hardness (r = -0.220, P>0.05) and calcium content (r = -0.289, P<0.05) of the related water supplies.It is concluded that the relationship between these associations may well be secondary. Analytical epidemiological studies of the association are called for.

Anencephaly↗