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Biomedical subjects

C Q Liu

Publications and source records attributed to C Q Liu.

44 records · Page 3Linked to original sources
PubMed1989

[Effects of epostane on chorio-trophoblast, co-culture of embryos with endometrium and corpora lutea in vitro].

Effects of epostane on human trophoblast cells, co-culture of mouse embryos with endometrium and guinea pig corpora lutea were studied in vitro. Human chorio-trophoblast cells were obtained from induced abortion (2-2.5 months of pregnancy). The tissue was incubated in control medium or in medium containing epostane. The results indicated that epostane at a concentration of 50 micrograms/ml could injure the human trophoblast cells and its function. Obviously damaged cells were observed at the concentration of 100 micrograms/ml. The cells were degenerated with pyknotic nuclei and sometimes only cell fragments were found. Guinea pig corpora lutea produced progesterone in vitro. In the incubation medium of the control group, the total luteal progesterone increased linearly with the prolongation of incubation during the first 30 min of incubation. The addition of hCG at concentration of 10 IU/ml clearly accelerated progesterone production. Epostane at a concentration of 50 micrograms/ml could significantly inhibit both basic and the hCG-stimulated luteal production of progesterone. Epostane at concentrations of 50 and 100 micrograms/ml showed no inhibitory effect on mouse embryos and endometrium in vitro.

Androstenols↗
PubMed1985

Effects of anordrin and its analogue on antifertility.

Anordrin has been used as an effective postcoital contraceptive in China. The mechanism of anordrin and its analogue SIPPR-113 on antifertility has been studied. Anordrin and SIPPR-113 possessed estrogenicities and induced decrease in serum progesterone levels in rats. Their antiprogesterone activities might be mainly caused by their estrogenicities, which were the main but not the only contributors for the antifertility. The direct effects of anordrin and SIPPR-113 on human trophoblast cells were studied. A concentration of 50 micrograms/ml or 100 micrograms/ml of anordrin or SIPPR-113 could injure the human trophoblast cells in vitro. The uterine Pontamine blue reaction of mated rats was inhibited in those treated with anordrin or SIPPR-113 at the dose of 4 mg/kg. Anordrin, SIPPR-113 or AF-45 was given orally, intramuscularly and intravenously. The effects of drugs administered via the three routes were nearly the same. This study further demonstrated that anordrin was hydrolyzed to break its bond of dipropionate and was transformed into its parent steroid AF-45 to exert its antifertility effects in vivo. This study warrants that anordrin should been evaluated further.

Administration, Oral↗
PubMed1983

Decline of follicular oocyte maturation inhibitor coincident with maturation and achievement of fertilizability of oocytes recovered at midcycle of gonadotropin-treated women.

To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with attainment of oocyte maturation and fertilizability, OMI was measured in follicular fluid (FF) of 39 follicles of 20 normal women given human menopausal gonadotrophin and human chorionic gonadotrophin to induce follicular growth and maturation. Oocytes were aspirated per laparoscope, the fluid was saved, and the egg was observed, incubated, and inseminated with the husband's sperm. Concepti that developed to the 4- to 8-cell stage were transferred to the uterus and the women were followed for pregnancy. OMI activity in each FF was measured by using cultured cumulus-enclosed porcine oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, and delta 4-androstenedione were measured in FF by radioimmunoassay. The FF of 13 preovulatory follicles yielding oocytes that were mature and fertilizable had significantly less OMI activity (mean +/- SEM) (0.58 +/- 0.10 unit/ml) compared to follicles yielding immature oocytes (2.8 +/- 0.56 units/ml; n = 9), atretic oocytes (5.5 +/- 2.5 units/ml; n = 7), or preovulatory oocytes with fractured zonae (1.9 +/- 0.63 units/ml; n = 7). The estrogen concentration (mean +/- SEM) of preovulatory follicles yielding mature fertilizable eggs or mature eggs with fractured zonae was greater (396 +/- 34 ng/ml; n = 20) compared to follicles yielding immature or atretic eggs (203 +/- 59 ng/ml; n = 9 and 97 +/- 47 ng/ml; n = 7, respectively; P less than 0.05). Progesterone concentration (mean +/- SEM; ng/ml) of FF was generally elevated in all preovulatory follicles (635 +/- 53) compared to immature or atretic follicles (230 +/- 64 and 76 +/- 17, respectively; P less than 0.05). It may be concluded that in normal follicle maturation there is a decline in OMI in the follicle containing an oocyte that becomes mature and fertilizable. There is also an increase in estrogen, progesterone, and follicle size. It is also possible to have an abnormal follicle maturation when there is an increase in size as well as FF, estrogen, and progesterone, but withut a decline in OMI--a situation which can lead to production of a nonfertilizable oocyte.

Adult↗