Mitotic stability of an episomal vector containing a human scaffold/matrix-attached region is provided by association with nuclear matrix.
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C Piechaczek
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A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.
We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.
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