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Biomedical subjects

C Petersen

Publications and source records attributed to C Petersen.

143 records · Page 8Linked to original sources
PubMed1985

[Short-term perioperative prophylaxis with cefotaxim in obstetric and gynecological surgery].

The effectiveness of short-term peri-operative prophylaxis with cefotaxim (Claforan) in gynaecological and obstetric operations was prospectively tested in 764 women (randomized by day of week). The incidence of postoperative infectious complications after vaginal or abdominal hysterectomy was significantly lower in the cefotaxim group than the control group. The rate was also clearly lower after laparotomy without hysterectomy and after abdominal section, but the differences were not statistically significant. In the patients with risk factors, i.e. obesity, cardiopulmonary disease or after laparotomy, which in the control group were correlated with a significantly higher rate of infectious complications, cefotaxim prophylaxis significantly reduced the rate of complications too. There were no side-effects from cefotaxim.

Cefotaxime↗
PubMed1977

[The Schwartz-Jampel syndrome (myotonia chondrodystrophica)].

Report on three siblings with typical features of Schwartz-Jampel's syndrome which is transmitted by a rare autosomal recessive gene: odd facies, stiff posture, high pitched voice, myotonic reaction, Perthes-like dysplasia of the femoral head. Biochemical and histological findings are minimal and nonspecific.

Abnormalities, Multiple↗
PubMedDate not supplied

The gene product of the Plasmodium falciparum 11.1 locus is a protein larger than one megadalton.

The Plasmodium falciparum 11.1 gene locus on chromosome 10 extends over 30 kb and contains approximately 22 kb of a tandemly repeated 27-bp sequence. Biochemical and size similarities have been noted between the reported 11.1 antigen and a variable-Mr, surface-radioiodinatable protein which appears to be involved in the cytoadherence of red blood cells infected with mature intraerythrocytic parasites to venular endothelium. We attempted to determine if these proteins were identical. Using rabbit antibody and affinity purified human immune sera specific for peptides encoded by the 27-bp repeat and a flanking 5' region, we have shown that the 11.1 locus encodes a protein of more than 1000 kDa. This protein cross-reacts with an uncharacterized 260-kDa protein, previously identified as the gene product of the 11.1 locus, and Pf155-RESA, but not with the radioiodinatable protein. The 11.1 protein that we have identified is a malarial protein of unprecedented size.

Animals↗
PubMedDate not supplied

[Central venous indwelling catheter systems in pediatric oncology from the surgical viewpoint].

A total of 307 central venous access devices were implanted by open surgery in 268 pediatric oncology patients. Their data were retrospectively evaluated from a surgical point of view. The cumulative duration of implantation of 116 subcutaneous tunneled Catheter (STC) was 12,206 days and 45,524 days for 191 portsystems. According to the duration in each group, ports were superior to STC when infection, complication and occlusion were taken into consideration. Infection of the ports was more frequent in children under 3 years than in older patients. Conversely, in the STC-group, children over 10 years presented with the highest rate of infection episodes. The number of complications was high in young children for both types of catheter, decreasing with age. Occlusion rates were similar for both systems and were not age related. However, thrombolysis was more successful in portsystems and few catheters had to be removed for this reason. The age-related data of the presented study are discussed with regard to the surgical procedure. Recommendations are made concerning the adequate choice of catheter system, the surgical technique and postoperative management. Additionally, the medicotechnical aspect of different catheter types is discussed and some suggestions and/or requests are directed to the industrial developing laboratories.

Bacteremia↗
PubMedDate not supplied

Characterization of a Cryptosporidium parvum sporozoite glycoprotein.

Polyclonal and monoclonal antibodies directed against Cryptosporidium oocysts or sporozoites were developed to identify and characterize sporozoite pellicle and apical complex antigens. A very large glycoprotein of Cryptosporidium sporozoites was identified by three monoclonal antibodies that also reacted with intracellular merozoites. The glycoprotein was also identified by polyclonal antibodies that were affinity-purified on nitrocellulose-bound recombinant proteins expressed by four lambda gtll genomic clones.

Animals↗
PubMedDate not supplied

Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins.

Microtubules and microfilaments are highly conserved cytoskeletal polymers hypothesized to play essential biomechanical roles in the unusual gliding motility of Apicomplexan zoites and in their invasion of, and development within, host epithelial cells. We have identified and isolated Cryptosporidium parvum genes encoding the microtubule proteins alpha- and beta-tubulin and the microfilament protein actin by screening a lambda gt11 C. parvum genomic DNA library with degenerate oligonucleotide and heterologous cDNA hybridization probes respectively. The alpha- and beta-tubulin genes have been partially sequenced and the deduced peptide sequences show greatest homology with the tubulins of the related parasites, T. gondii and P. falciparum. The complete nucleic acid sequence of the actin gene predicts a 376 amino acid, 42 kDa protein having 85% sequence identity with the P. falciparum actin I and the human gamma-actin proteins. Each of these cytoskeletal protein genes was demonstrated to be of cryptosporidial origin by Southern analyses of C. parvum chromosomes fractionated by pulsed field gel electrophoresis; the cloned alpha- and beta-tubulin genes hybridized with chromosomes of ca. 1,200 and 1,500 kb respectively and the cloned actin gene also hybridized with a 1,200 kb chromosome.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Amplification of a Cryptosporidium parvum gene fragment encoding thymidylate synthase.

Currently, there is no effective therapy for cryptosporidiosis and it is unclear why antifolate drugs which are effective treatments for infections caused by closely related parasites are not also effective against Cryptosporidium parvum. In protozoa, the target of these drugs, dihydrofolate reductase (DHFR), exists as a bifunctional enzyme also manifesting thymidylate synthase (TS) activity and is encoded by a fused DHFR-TS gene. In order to prepare a probe to isolate the C. parvum DHFR-TS gene we have used degenerate oligonucleotides whose sequences are based on strongly conserved regions of TS protein sequence to prime the polymerase chain reaction (PCR) with C. parvum DNA. The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues. The cloned PCR fragment was used as a probe to isolate a number of overlapping clones from a C. parvum genomic library which were definitively shown to be of cryptosporidial origin by genomic Southern and molecular karyotype analyses. The deduced protein sequence of C. parvum TS was most similar to the bifunctional TS enzymes of Plasmodium chabaudi and Plasmodium falciparum.

Amino Acid Sequence↗
PubMedDate not supplied

Cryptosporidium parvum: in vitro cultivation in Madin-Darby canine kidney cells.

To facilitate studies of the biology of Cryptosporidium parvum, we have developed an in vitro culture system using Madin-Darby canine kidney (MDCK) cells as the host cell. Oocysts or free sporozoites were incubated 37 degrees C with monolayers of MDCK cells in supplemented RPMI 1640 medium and the cells were examined at various time intervals after initiation of the culture. High rates of infection (up to 90% of MDCK cells) were achievable. Sequential development of trophozoites, meronts, microgametocytes, and macrogametocytes was observed over a 72-h period of culture. Between 72 and 96 h we observed formation of oocyst walls, but fully sporulated oocysts were not observed. This culture system provides access to both the asexual and sexual intracellular stages of C. parvum.

Animals↗