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Biomedical subjects

C Parmentier

Publications and source records attributed to C Parmentier.

At least 181 records · Page 10Linked to original sources
PubMed1979

The suicide technique in vitro for granulocytic progenitor cells (CFC) by application of hydroxyurea (HU) to low concentrations of human bone marrow cells.

A suicide technique applicable to human bone marrow granulocytic progenitor cells has been previously described (3). This study discusses the conditions for its use on bone marrow aspirates poor in cells. When the number of cells exposed to hydroxyurea (HU) is proportional to the quantity of HU it is possible to avoid artifacts due to HU toxicity. This technique can repeatedly reproduce a 40--50% suicide. To obtain this result we suggest using a 6.10(-6) mM/ml/cell HU concentration.

Bone Marrow↗
PubMed1979

Enrichment of murine granulomonocytic progenitors using a continuous linear Ficoll-Isopaque gradient.

Murine bone marrow was fractionated using a Ficoll-Isopaque continuous linear gradient characterized by an osmolarity of 290 mOsm constant throughout the gradient and a physiological pH of 7.4. The cellular peak detected prior to fractionation by means of a 405 nm light beam served as a guide for determining fraction collection. Under these conditions CFC enrichment was observed constantly for densities lower than that of the cellular peak. In 17 experiments the enrichment factor amounted to 3.6 +/- 1.4. Enrichment appeared to be due to both an increase in CFC concentration and an improvement in CFC cloning efficiency. A correlation between the concentration of CFC and that of undifferentiated blasts was observed. The CFC density distribution was dependent on the cell load. For cell loads lower than 25 x 10(6) the modal density of CFC was within the range 1.0615-1.0715. For cell loads higher than 25 x 10(6) there was a shift of the distribution curve towards higher density. Clu-CFC appeared to be denser than CFC. This increase in density may be due to a maturation process from CFC to Clu-FC as a maturation gradient with increasing density was found for the granulocytic series and for erythroblasts.

Animals↗
PubMed1979

Methylcellulose culture of human granulocytic progenitor cells (CFC *): results of bone marrow and blood cultures for normal subjects.

Normal values for the colony-forming ability of granulocytic progenitor cells (CFC) were established for 26 samples of normal bone marrow and 8 samples of normal blood cultured in methylcellulose. The concentration of CFC in the marrow was measured classically as the number of CFC per 10(5) nucleated marrow cells plated and, in addition, as the number of CFC per 10(5) precursors (myeloblasts, promyelocytes, and myelocytes) and per 10(5) metamyelocytes. A value approximating the total number of marrow CFC was calculated by using the number of CFC per 10(5) metamyelocytes. The normal value for circulating CFC were determined as number per ml of blood, per kg of body weight, per 10(5) circulating lymphocytes, and per 10(5) circulating granulocytes. The value of these different modes of expression are discussed. Although the values reported are not directly applicable from one laboratory to the next, they may constitute a useful reference point. Furthermore, they show that the statistical variation in the results is sufficiently small to permit the comparison of values found for pathologic samples with those determined for normal samples.

Adult↗
PubMed1979

Sequential study of the bone marrow granulocytic progenitor cells (CFC *) in children treated by chemotherapy for non-hodgkin malignant lymphomas.

We have studied the time course changes in committed granulocytic progenitor cells (CFC) in 54 pediatric patients treated by chemotherapy for a malignant non-Hodgkin lymphoma. This study has revealed the following points: 1) In the absence of patent bone marrow invasion, the bone marrow CFC concentration is, before treatment, less than normal; 2) Bone marrow invasion coincides with a large decline in bone marrow CFC concentration; 3) The aplasia induced by the initial chemotherapy is mediated by a quasi-complete destruction of CFC; 4) After regeneration from the initial therapeutic aplastic state the CFC exhibited a quite variable behavior from one patient to another. This phenomenon is probably linked to the variable recuperation time for each patient; 5) Following the conclusion of treatment, the patients, although having a normal peripheral leukocyte count, appeared to maintain a major aftereffect characterized by a clear and prolonged reduction in the concentration and total number of CFC.

Adolescent↗
PubMed1978

[Suppression of hepatic myeloid metaplasia after irradiation of the spleen in a case of spent polycythemia (author's transl)].

In a case of spent polycythemia after irradiation of the spleen alone (450 rad in 17 sessions and 28 days) the suppression of any hepatic myeloid metaplasia was observed simultaneously with the regression of myelemia and erythroblastemia and the drop in the number of circulating granulomonocytic stem cells. These observations allow to discuss the mode of action of the splenic radiotherapy and the relationship between myeloid metaplasia and the circulating hematopoietic cells (proliferating granulocytic and erythrocytic cells and stem cells).

Bone Marrow Cells↗
PubMed1978

[Ineffective erythropoiesis in myeloid splenomegaly: the 59Fe test, bone marrow histologic and cytologic data].

Eight patients presenting primary myelofibrosis or spent polycythemia were submitted to a ferrokinetic study, a triphine bone marrow biopsy and a bone marrow puncture. In all cases before treatment (splenic radiotherapy) ferrokinetic measurements demonstrated an ineffective erythropoiesis, the intensity of which was precised on bone marrow biopsies, in numbering the erythroblasts per surface unit. Bone marrow biopsies permitted also, through a semiquantitative estimate of the bone marrow cellularity, to evaluate an aplasia component not always expected with ferrokinetic study. Study of bone marrow allowed to precise the site of the erythroblastic abortion; cellular death might occur during the last nitosis of the erythroblastic series (transition from the polychromatophil erythroblasts to the acidophil erythroblasts). In order to delineate the general pattern of erythropoiesis before treatment and when assessing the results of a treatment, besides ferrokinetic measurements, the study of bone marrow biopsy and smears appears relevant.

Adult↗
PubMed1978

Complications of total abdominal and spleen irradiation in patients with lymphomas.

Fifty patients with non-Hodgkin's lymphoma were treated with total abdominal irradiation to a dose of 3000 rad by anterior and posterior fields treated the same day. Fourteen patients developed complications, four with intestinal obstruction due to stenosis and three with total or partial intestinal irradiation damage. These bowel complications were only seen in patients who had previously had a laparotomy. The ways of preventing these complications are discussed. We have studied renal function by means of clinical examination, biologic and radiologic investigations, and scanning in 75 patients who have received splenic irradiation to a dose of 4000 rad. With a mean follow-up time of 36 months, this study has shown that there was no significant change in blood pressure or in biochemical measurements. However, at the 17th month, nephrotomograms demonstrated cortical atrophy of the upper role of the kidney; at the 8th month, low uptake in the irradiated area was seen on 197Hg neohydrine scanning and a decrease in renal plasma flow in the left kidney during dynamic studies with Hippuran.

Abdomen↗
PubMed1978

Effects of treatment of malignant lymphomas on the granulocytic progenitor cells (CFUc).

The goal of this work was to appraise the after-effects of radiotherapy and associated radio- and chemotherapy on hematosarcomas by studying the granulocytic progenitor cells (CFUc). The bone marrow of 14 subjects was cultured in methylcellulose 1--6.5 years after completion of radiotherapy. The results were recorded as the number of CFUc per 10(5) nucleated medullary cells plated and per 10(5) metamyelocytes plated, and the total number of medullary CFUc was calculated. These different modes of expression are discussed. The post-radiotherapeutic effects appeared to be closely correlated with the volum irradiated. Chemotherapy seemed in several cases to accentuate the effects. Nevertheless, the organism can operate a powerful compensatory mechanism by means of increased mitosis, and in addition, certain subjects are able to maintain a normal number of CFUc after an aggressive treatment. It was possible in certain cases to study the medullary regeneration of the irradiated zone by culture of the CFUc and by the 59Fe external counting. Its extent after radiotherapy seemed for both the granulocytic and the red cell line to be correlated with the volume irradiated. Subject to the study of new cases, regeneration appears to be very reduced when chemotherapy is associated with radiotherapy.

Antineoplastic Agents↗
PubMed1978

Ways of expressing results of human bone marrow progenitor cell culture.

In human bone marrow culture studies the number of granulocyte progenitor cells (CFUc) is expressed as the number of colonies per 10(5) incubated, nucleated cells. However, this provides little information on cell proliferation kinetics of granulopoiesis. The ratio of progenitors (CFUc) to end cells (metamyelocytes or granulocytes) is related to the effectiveness of granulopoiesis and this allows an estimate to be made of an amplification factor within the maturing and proliferating compartments. We measured an index equal to the number of CFUc per 10(5) falls when a large number of granulocytes is produced by each progenitor cell. This was observed in many patients with a neutropenia. An estimation of the total number of metamyelocytes is based on the ratio of the number of metamyelocytes to the number of blood granulocytes. This allows an estimate of the total number of CFUcs, which is of clinical interest.

Bone Marrow Cells↗
PubMed1978

In vitro medullary granulocytic progenitor (CFUc) cultures from 6 cases of granulocytopenias.

Medullary granulocyte progenitor (CFUc) cultures were grown in vitro from samples taken from 6 patients with toxic granulocytopenia caused by either thiamphenicol cephalothin or amidopyrine and who are now apparently cured. A decreased in the medullary concentration of CFUc has been observed and a calculated estimate shows that there was a decrease in their absolute number. A decrease in the number of CFUc per 10(5) metamyelocytes suggests a possible compensation by mitotic amplification between the stem cell and the differentiated cells. Two successive cultures have shown that the course of such medullary cultures is variable. The existence of medullary anomalies before drug toxicity as well as the practical consequences of the contrast between the apparent cure and the decrease in CFUc are discussed.

Adult↗
PubMed1977

[Interest of sequential "in vitro" cultures of granulomonocytic stem cells (CFUc) in the management of chemotherapy in a case of multiple myeloma (author's transl)].

Sequential measures of granulomonocytic stem cell (CFUc) pool were undertaken during chemotherapy in a case of multiple myeloma. Whereas the number of blood granulocytes remained unchanged, the total number of CFUc was markedly decreased. Therefore the CFUc marrow pool can be considered as one of the data in the management of chemotherapy. In multiple myeloma, the tumour mass can be evaluated by the secretion of monoclonal lg. It appears possible to adapt the schedule of chemotherapy considering both its effect against the tumoral cells and its toxicity on bone marrow stem cells.

Bone Marrow Cells↗
PubMed1977

Detection of hepatoma in liver cirrhosis.

The analogic liver scintigram using 99mTc sulfur colloid in cirrhotic patients does not permit determination of the nature of the areas of decreased uptake. Scintigrams with 67Ga citrate generally show increased activity in cases of hepatoma. In some cases, however, 67Ga citrate is less concentrated in neoplasic tissue, and it is not possible to detect a tumoral lesion in a cirrhotic liver. This is why we used double isotope scintigraphies with 67Ga citrate and 99mTc sulfur colloid, with digital subtraction, after simultaneous recording of 99mTc and 67Ga data on magnetic tape by means of an interface. In our series of 22 patients, the comparison of the results obtained by this double isotope technique with histology showed no false positive in substraction scintigrams. There was one false negative because of the lack of significance in the subtracted image for one of the six patients with cancer of the liver. For three of the six patients with hepatoma, the gallium scintigram showed an increased uptake in the tumor area. For the three other cases, the gallium uptake was equilibrated throughout the liver scintigraphy. It was therefore in cases where the gallium scintigram showed no increased activity that the subtraction technique was of greatest value, for it permitted the diagnosis of hepatoma in two cases.

Carcinoma, Hepatocellular↗
PubMed1977

Comparative study of 111In and 59Fe bone marrow scanning.

The aim of this work is to examine the relative merits of 59Fe and 111In scanning in estimating the functional value of the various erythropoietic areas and in appraising bone marrow distribution and extension. At first we studied a group of patients with heterogeneous distribution of bone marrow secondary to irradiation, ferrokinetics serving a reference for the comparison of 111In and 59Fe scanning. The functional value fo 59Fe scanning was thus proved, in contrast to 111In scanning which was erroneous for 13 of the 29 bone marrow areas studied. A further study of patients presenting polycythemia vera or anemia with 111In demonstrated in some cases false distribution and bone marrow extension. Furthermore, 59Fe scanning permitted evaluation of the efficiency of erythropoiesis and the existence of extramedullary erythropoiesis. Review of the literature revealed that with the activity used there was no dosimetric advantage of 111In over 59Fe.

Anemia↗
PubMed1977

Description of a suicide technique in vitro for granulocytic stem cells (CFUc) by hydroxyurea on normal human bone marrow.

It has been possible, by studying 15 normal human bone marrow samples, to develop a technique for the suicide effect in vitro by hydroxyurea (H.U.), applied to human granulocytic stem cells (CFUc). This study suggests a contact time of medullary cells from 60 to 80 minutes in the presence of a hydroxyurea concentration 6 mM per 10(6) cells per ml in vitro. Preliminary results concerning the clusters are discussed.

Bone Marrow↗
PubMed1977

[In vitro granulocyte colony formation in children with neuroblastoma (author's transl)].

In vitro granulocyte colony formation has been studied using the methylcellulose system in 22 children with neuroblastoma, 13 of them having bone marrow invasion. Only one third exhibited normal results. There were various disturbances of granulopoiesis in vitro: colony forming cells were decreased or increased and in the amplification compartment either an ineffective leucopoiesis or an increase in the number of mitosis was present. The abnormalities are not correlated to bone marrow invasion.

Blood Cell Count↗