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Biomedical subjects

C P Douglas

Publications and source records attributed to C P Douglas.

At least 19 recordsLinked to original sources
PubMed1994

Evaluation of E test as a rapid method for determining MICs for nutritionally variant streptococci.

E test was evaluated as an alternative rapid and simple method of MIC estimation for nutritionally variant streptococci. E test with various media was compared with conventional broth and plate dilution techniques supplemented with 0.001% (wt/vol) pyridoxal hydrochloride (vitamin B6). Of the 14 strains tested with E test, isosensitest agar supplemented with 5% defibrinated horse blood and 0.001% pyridoxal HCl, with and without 0.01% cysteine, gave complete agreement within one twofold-dilution titer of the agar reference method and between 93 and 86% agreement within one twofold-dilution titer of the broth reference method. E test MICs with other media were comparable; however, these were considerably more difficult to interpret. Use of Mueller-Hinton and Columbia-based supplemented agar showed hazy growth and double zoning around the endpoint, respectively. The addition of 0.01% (wt/vol) cysteine to media exhibited no significant effect, and incubation in 5% carbon dioxide (CO2) did not affect MICs.

Agar↗
PubMed1984

A regimen for obtaining mature human oocytes from donors for research into human fertilization in vitro.

Patients awaiting laparoscopic sterilization were canvassed to allow oocytes to be aspirated from their ovaries at the time of operation for the purpose of testing the fertilizing capacity of spermatozoa from clinically infertile men. Because these patients were volunteers, the standard techniques for stimulation, ovulation prediction, operation, and oocyte recovery had to be modified for maximum safety and for convenience to both patient and staff. Superovulation was induced with 100 mg or 150 mg clomiphene citrate on days 2 to 6 or 5 to 9 of the menstrual cycle. Final maturation of oocytes was stimulated with 5000 IU of human chorionic gonadotropin administered between days 11 and 15 of the cycle, and the operation was performed 34 to 36 hours later on the routine gynecology operation lists. Of the 157 patients canvassed , 47% were willing to donate oocytes to the project. Despite the variability of the stimulation regimens, the simplified operative technique, and the fact that more than half the patients were on oral contraceptives in the previous cycle, one or more oocytes were retrieved in 75% of the patients. At the initial assessment, 83% of these were considered suitable for in vitro fertilization.

Adult↗
PubMed1982

Low birth weight babies. Preterm and small: the obstetric challenge.

Preterm labour is of unexpected origin, closely related to social class and a history of similar earlier obstetric situations. If maturity is more than 34 weeks the hazards are not great and can be comfortably cared for by paediatricians. Stopping labour can cause harm, either to the fetus which is in distress or to the mother who may not benefit from the drugs used to stop it. Thus not only can we both harm the fetus by stopping labour, even if the drugs are totally safe, but also with present methods we cannot be assured of absolute intrauterine fetal wellbeing. A decision to stop labour should therefore be confined to two groups of patients. First, the patients who would be better transferred to an appropriate intensive care neonatal unit (and there is no doubt that intrauterine transfer is preferable to extrauterine transfer because of the importance in hypoxia of the first 20 minutes). Secondly, to patients who are in preterm labour without any good cause, where the mother is totally healthy, the fetus is healthy, alive, and not at evident risk, the gestation is less than 35 weeks, the baby weighs less than 1500 g or more than 600 g, the membranes are intact, the cervix is less than 3 cm dilated, and there is no obvious intrauterine growth retardation.

Breech Presentation↗
PubMed1970

Xylene poisoning: a report on one fatal case and two cases of recovery after prolonged unconsciousness.

Three cases of xylene poisoning occurred after prolonged inhalation of paint fumes. Analysis showed that xylene comprised more than 90% of the solvent in the paint, the total solvent comprising 34% of the paint by weight. One patient was dead on admission, while the other two recovered after at least 15 hours' loss of consciousness. Both patients had transient liver cell damage, and one temporary impairment of renal function.

Adult↗