Search PubMed⌕ Search

Biomedical subjects

C Motta

Publications and source records attributed to C Motta.

At least 73 records · Page 4Linked to original sources
PubMed1982

[Various amniotic studies in the assessment of fetal pulmonary maturity].

The need for knowledge concerning foetal pulmonary maturity has led biochemists to develop a number of techniques based upon knowledge of amniotic phospholipids. Study of these phospholipids of foetal pulmonary origin is possible using biochemical and physical methods. The former include the L/S (Lecithin/sphingomyelins) ratio, as well as the measurement of total lecithins, or the palmitic acid/stearic acid ratio (P/S). Physical methods are based upon surface tension (Clements test) or fluidity (measurement of microviscosity by polarization of fluorescence) properties of phospholipids. Combination of several reproducible, sensitive and reliable tests (E/S ratio, microviscosity, Clements test) provides adequate information concerning foetal pulmonary maturity.

Amniotic Fluid↗
PubMed1981

Phospholipids and microviscosity study in broncho-alveolar lavage fluids from control subjects and from patients with extrinsic allergic alveolitis.

Bronchoalveolar lavages were studied in control subjects and patients with extrinsic allergic alveolitis. Phospholipids, fatty acid composition of the different phospholipid species found and cholesterol were investigated. In allergic alveolitis, the phosphatidylcholine was absent, whereas two usually minor components in lung surfactant, phosphatidylethanolamine and phosphatidylinositol, were considerably enhanced. The tensio-active properties of the phospholipids present in fluids were investigated by a fluorescence polarization technique. Microviscosity of the phospholipids was studied, using 1,6-diphenyl-1,3,5-hexatriene. High values for the microviscosity were encountered in samples from patients. Phosphatidylethanolamine present at a high level exhibits the highest microviscosity. Saturated fatty acids in phospholipids and cholesterol are rigidifying factors contributing to the high microviscosity values found in allergic alveolitis. Moreover, one very important point microviscosity values found in allergic alveolitis. Moreover, one very important point brought out by the this technique is its full independence from the fluid volume recovery.

Adult↗
PubMed1980

A rapid and sensitive fluorometric assay of serum phospholipid.

A fluorometric assay of phospholipid in serum is presented. The reaction is based upon the binding of phospholipids with a fluorescent probe: 1-6-Diphenyl-1-3-5 hexatriene (DPH). The method is in very good agreement with other techniques for phospholipid estimation: phosphorus assay phospholipid extraction (Fiske and Subarrow), and enzymatic assay of phospholipid containing choline (Takayama). The correlation coefficient obtained was 0.81, when the proposed method was compared with the two other ones. Parameters influencing the reaction: DPH concentration, time and temperature of incubation are described. The normal range for human serum lies between 2.1 and 3.4 mmol/l. The sensitivity of the fluorometric assay is also sufficient for the determination of phospholipid in amniotic fluid and broncho-alveolar washings.

Amniotic Fluid↗
PubMed1979

[Foetal pulmonary maturity: estimation by the palmitic/stearic acids ratio and by the Clements' test in amniotic fluid (author's transl)].

Two parameters have been used to estimate the foetal pulmonary maturity: Clements' test or bubble test and palmitic acid/stearic acid ration. The reliability and the limits of these tests have been studied in function of several pathological pregnancies and the gestations's age. A value greater than 5 for the P/S ratio was a good pronostic at any gestational age. A value lesser than 5 should be examined according to this gestational age. A negative Clements' test has to be confirmed by an another amniocentesis, while a positive test have been followed always by a mature newborn's birth.

Amniocentesis↗
PubMed1979

[Lipids and phospholipids in the fluid of broncho-alveolar lavage. Value for the study of alveolar penetration of corticosteroids by aerosol (author's transl)].

Broncho-alveolar lavages from 13 control subjects have been studied for the following parameters : phospholipids, free fatty acids, total last parameter, through the depolarisation of Diphenylhexatriene, is independent of the recovered volume. An identical study has been performed for two bronchiolo-alveolar cancers. A treatment with hydrocortisone-containing aerosol has been associated with an increase in phospholipid amount and a drastic decrease in microviscosity. This observation suggests that the synthesis of tensio-active phospholipids is dependant of corticoids by an enzymatic induction. The effect of therapeutics given in aerosol can be monitored by the biochemical and physical modification in broncho-alveolar fluids thus obtained.

Adrenal Cortex Hormones↗