Biomedical subjects

C Mechem

Publications and source records attributed to C Mechem.

3 recordsLinked to original sources

PubMed1994

Improved left ventriculography with the new 5F helical-tip Halo catheter.

The purpose of this study was to evaluate the incidence of ventricular ectopy and catheter movement during left ventriculography with a new 5F halo angiographic catheter that has a unique helical-tip design unlike the design of standard 5F and 6F pigtail catheters. The pigtail catheter is presently preferred for left ventriculography, although its use is associated with a high incidence of ventricular ectopy, which often limits precise interpretation of data. In this study, 155 patients (in 145 unpaired and 10 paired studies) underwent left ventriculography during diagnostic cardiac catheterization. In the unpaired group, the 5F Halo catheter was used in 63 studies and standard 5F and 6F pigtail catheters in 40 and 42 studies, respectively. An additional 10 patients had two consecutive left ventriculograms with 5F Halo and pigtail catheters. Ventriculograms were performed with the same technique in the 30-degree right anterior oblique projection. The left ventricle was divided into a basal zone, midzone, and apical zone. Catheter movement within the ventricle was scored as significant if there was at least one zone change. Ventricular ectopy was quantified by a simultaneous electrocardiographic recording during contrast injection. There were no significant differences in the left ventricular systolic or end-diastolic pressures, left ventricular score, or diagnostic quality of the ventriculograms between the 5F Halo catheter group and the 5F and 6F pigtail catheter groups. Mean ventricular ectopy with the 5F Halo catheter was significantly less (0.9 +/- 1.4 ventricular premature beats [VPBs]) than with the 5F pigtail catheter (2.3 +/- 2.5 VPBs, p < 0.001) or the 6F pigtail catheter (2.9 +/- 2.9 VPBs, p < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗

PubMed1994

Wound botulism.

Wound botulism is a rare infectious and toxicologic complication of trauma and i.v. drug abuse. Only 39 cases have been reported in detail in the English literature. This case report describes a patient with wound botulism who presented to four medical facilities before receiving definitive diagnosis and treatment. Although his history and physical examination were consistent with wound botulism, diagnosis and therapy were delayed because this rare disease was not considered initially in the differential diagnosis. Wound botulism should be considered in trauma patients and i.v. drug abusers who present with cranial nerve palsies and descending paresis.

Abscess↗

PubMed1989

Intravital fluorescence microscopy of endothelial cells on vascular grafts.

The ability to evaluate the extent of initial endothelial cell coverage on a vascular graft subsequent to an endothelial cell seeding technique would be desirable to substantiate the durability of the endothelial cell lining. To this end, we have evaluated the use of intravital fluorescence microscopy to assess human endothelial cell interaction with vascular grafts. Five fluorescent stains, mithramycin, Hoechst 33342, sulfofluorescein diacetate, Nile red, and rhodamine 123 were evaluated for their ability to fluorescently label human endothelial cells. The staining capability of each dye was also evaluated with respect to accuracy in determining seeded cell number and cell spreading. Of the stains evaluated, rhodamine 123 produced the most desirable characteristics. We observed excellent cell visualization after a 30-min incubation. Unlike the other four stains, rhodamine 123 exhibited a bright orange fluorescence emission at a 510-nm excitation wavelength while the underlying dacron or expanded polytetrafluoroethylene demonstrated minimal autofluorescence. Rhodamine 123 also exhibited no inhibitory effect on cell attachment to plastic or subsequent cell growth in culture. Intravital fluorescence microscopy could be easily utilized in the operating room to visualize part or all of an endothelial cell-seeded graft prior to implantation and would permit a quantitative as well as qualitative evaluation of the seeding process. Since intravital fluorescence imaging does not require tissue fixation, the same surface as that evaluated for seeding efficiency can be directly implanted.

Blood Vessels↗