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Biomedical subjects

C Marchal

Publications and source records attributed to C Marchal.

At least 145 records · Page 8Linked to original sources
PubMed1982

[The detection of deep venous thrombosis by fibrinogen labelled with iodine 125. Specificity and sensibility in comparison with standard phlebography (author's transl)].

During the last 6 years, more than 4,000 tests using 125I fibrinogen have been performed on orthopedic and traumatic patients who were undergoing a thromboembolic treatment by means of subcutaneous heparin. The high accuracy of this method has been confirmed using phlebography. This test still remains a simple, faithful, and non traumatic diagnosis tool in the early detection of thrombosis of the limbs of patients undergoing traumatologic and orthopedic surgery. Moreover, the labeled fibrinogen test is useful in the determination of the efficiency of antithrombotic preventive therapy.

Diagnosis, Differential↗
PubMed1982

Ionic competition and 67Ga in vivo accumulation.

The effect of several citrates of trivalent cations on 67Ga citrate uptake by tumor-bearing animals has been studied. A relationship between the ionic size of the simultaneously injected cation and the 67Ga uptake inhibition indicates that the cation characteristics (ionic radius and electrical charge) seem to play the most important role in this radioactivity accumulation. The apparent agreement of this phenomenon with the isomorphous replacement hypothesis is discussed.

Animals↗
PubMed1981

[Myocardial scintigraphy using technetium-99m labelled methylene diphosphonate in coronary care units. 82 cases].

Many isotopic methods have been proposed recently for the investigation of normal and diseased myocardium in ischaemic heart disease. The results of myocardial scintigraphy with Technetium 99 m marked methylene diphosphonate in the supine, left lateral and 30 degrees or 45 degrees right anterior oblique incidences are reported. A preliminary study of the tracer's fixation on diseased myocardium was performed in 18 patients. Scintigraphy every 30 minutes over a five hour period showed the optimal time for investigation to be situated between the third and fourth hour after the injection of the tracer. Seventy eight patients were then investigated; 82 scintigraphies were performed, 58 in patients with acute myocardial infarctions confirmed by the usual biological and electrocardiographic changes. A 74% sensitivity and 67% specificity were obtained with the methylene diphosphonate method. These figures varied according to the type of necrosis, subendocardial or transmural and with its extent, chronicity and degree of CPK elevation. Methylene diphosphonate scintigraphy would appear to be less sensitive and more specific for diseased myocardium than other isotopic methods. It may be carried out at the bedside and could improve the diagnosis of acute myocardial infarction when the clinical history and electrocardiographic changes are difficult to interpret.

Adult↗
PubMed1981

[Combined hyperthermia, radiotherapy and chemotherapy: preliminary clinical results (author's transl)].

Results of treating superficial cancerous nodules by hyperthermia, alone or combined with radiotherapy or chemotherapy, over a period of one year, are presented. Twenty-five patients with multiple cutaneous or subcutaneous metastatic tumors were treated by 1 to 3 MHz ultrasound hyperthermia or 434 MHz microwave hyperthermia. Good local tolerance was observed, even in regions previously treated with high doses of irradiation. Regression of the tumors was assessed by comparison with unheated nodules. Two out of 11 tumors treated by hyperthermia alone, 10 out of 17 tumors treated by conventional chemotherapy combined with hyperthermia showed significant regression.

Hot Temperature↗
PubMed1980

Synthesis and maturation of lambda receptor in Escherichia coli K-12: in vivo and in vitro expression of gene lamB under lac promoter control.

The lambda receptor is an outer membrane protein from Escherichia coli K-12 lamB, its structural gene, is part of the maltose regulon. We have cloned this gene in a phage so that it is under the control of the lac promoter. The phage was devised in such a way that it can infect lamB mutants and that chromosomal lamB mutations can be transferred to it. In vivo, the lambda receptor is expressed under lac promoter control and is exported normally to the outer membrane, independently of the expression of the other genes of the maltose regulon. In vitro, DNA of the phage allows efficient synthesis of the lamB product. The protein--or pre-lambda-receptor--made in vitro contains an NH2-terminal sequence of about 25 amino acids not found in the lambda receptor. We have detected no inactivation of phage lambda by the pre-lambda-receptor. Conversion of the pre-lambda-receptor to a form that has the apparent molecular weight of the mature lambda receptor was achieved. A lamB mutation that blocks export in vivo also blocks conversion in vitro.

Bacterial Proteins↗
PubMed1980

DNA sequence encoding the NH2-terminal peptide involved in transport of lambda receptor, an Escherichia coli secretory protein.

lamB encodes the lambda receptor of Escherichia coli, an outer membrane protein. We have identified the beginning of the lamB gene by correlating DNA nucleotide sequence with a partial sequence of the primary translation product of lamB. We show that lambda receptor is synthesized as a precursor containing an extra 25 amino acids at its NH2 terminus. These amino acids are predominately hydrophobic and probably comprise a structure required for initiation of transport of lambda receptor from the cytoplasm to the outer membrane.

Amino Acid Sequence↗
PubMed1980

[Atypical meningocele localization in the new-born. A report on one case (author's transl)].

A case is reported of a new-born infant presenting with a voluminous laterofacial tumor which had literally blown up the right side of the face. The tumor was found to be a meningocele. Treatment was applied in such a way as to cause the least surgical trauma possible and to use the natural flexibility of the tissues in an infant of this age. The rare nature of this localization is emphasized.

Encephalocele↗
PubMed1980

[Carotid artery investigation by real time ultrasonic imaging (author's transl)].

Ultrasonography examination enables vascular lesions in the main cervical arteries to be relatively easily recognized in patients with hemodynamic disturbances, but these changes are no longer present when the vascular anomalies are not functional. A non-invasive method for detailed visualization of the carotid axis was developed, by the use of rapid continuous ultrasonography. This can reveal the presence of unsuspected atheromatous plaques during the stage when prevention is still possible, and can complement certain investigations of doubtful value that had suggested that atheromatous plaques existed.

Carotid Artery Diseases↗
PubMed1980

[Neonatal hyperparathyroidism secondary to maternal hypoparathyroidism (author's transl)].

Three cases of neonatal hyperparathyroidism are reported: 2 children born to a mother with idiopathic hypoparathyroidism and a boy born to a mother with pseudohypoparathyroidism. Severe demineralization, decreased plasma calcium levels and very low phosphorus levels were seen in all three cases. The PTH level was high in the two cases in which is was measured. In the second of these, the lack of vitamin D administration since birth led to very severe rickets lesions at the age of 7 weeks. This is an argument for the role of hyperparathyroidism-induced vitamin depletion in the lesions observed and in the maintenance of secondary hyperparathyroidism. Analysis of 10 previously reported cases reveals the severity of the calcium deficiency induced in the fetus in this manner, the frequency of prematurity, of dysmaturity. With calcium and vitamin treatment, biological and radiological findings become normal.

Adult↗
PubMed1979

Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter. Mutations Qam73 and Sam7 greatly increase the amount of beta-galactosidase synthesized by the vector bacteriophage. The lambda ZEQS vector has been certified B2 (EK2) by the French control commission "Recombinaisons génétiques in vitro".

Coliphages↗
PubMed1979

[A vector system designed to allow excretion of a protein encoded by a cloned gene].

We describe a method which should allow construction of bacterial strains able to export a given protein through the cytoplasmic membrane. The principle is to fuse the structural gene of the protein to the proximal part of gene lamB, the structural gene of the lambda receptor, an outer membrane protein of E. coli K-12.

Bacterial Proteins↗
PubMed1978

malB region in Escherichia coli K-12: specialized transducing bacteriophages and first restriction map.

By starting from an Escherichia coli K-12 strain with a lambda phage integrated in the malB region, series of transducing phages carrying part or all of the malB region have been isolated. Genetic mapping of the transduced malB fragments was accomplished by complementation and recombination with known mutations in the region. By using the DNA of these phages, it was found that the malB region is cleaved by the restriction enzymes BglII, EcoRI, HaeII, HincII, SalI, and SstI, but not BamHI, HindIII, KpnI, PstI, XbaI, or XhoI. A physical map was constructed and tentatively correlated with the genetic map.

Chromosome Mapping↗