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Biomedical subjects

C Maśliński

Publications and source records attributed to C Maśliński.

At least 19 recordsLinked to original sources
PubMed1993

The influence of Tołpa Peat Preparation (TPP) on rat liver regeneration.

The effect of Tolpa peat preparation (TPP) on the regenerative response has been examined in rats submitted to two thirds hepatectomy. The ornithine decarboxylase activity, spermidine and histamine levels, DNA and RNA content, RNA/DNA ratio and the mass of restituted liver were used to test the intensity of the regenerative processes. The action of TPP is dual: a short-term application of TPP at a dose of 20 mg/kg/day causes an inhibition of the ornithine decarboxylase activity, the decrease in spermidine formation, the levels of DNA and RNA, and liver restitution. The multiple application of TPP, on the other hand, results in a stimulation of ornithine decarboxylase, the increase in spermidine and histamine as well as RNA and DNA levels in regenerating liver; concomitantly, the liver mass tends to increase in TPP treated groups. TPP might exert its effects, at least partially, by interfering with polyamine biosynthesis.

Amino Acids↗
PubMed1993

Investigations on allergogenic properties of Tołpa Peat Preparation.

The ability of Tołpa Peat Preparation (TPP) to induce or enhance an allergic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera and IgG1 as well as IgE antibody levels in guinea pig sera were evaluated by PCA (Passive Cutaneous Anaphylaxis) tests. TPP adsorbed on aluminium hydroxide gel (alum) and introduced into BALB/c mice by several subcutaneous injections was unable to stimulate the noticeable anti-TPP IgE antibody response. TPP introduced together with ovalbumin (OA) into the mice in the course of immunization with OA did not enhance anti-OA IgE antibody response. TPP adsorbed on alum and injected subcutaneously into guinea pigs was unable to induce noticeable IgG1a, IgG1b and IgE antibody response, and mast cells obtained from lung and mesentery of these animals did not release histamine when challenged with TPP in vitro at 37 degrees C. In conclusion, our results show that under the experimental conditions used in the present experiments TPP was unable to induce or enhance an allergic sensitization of mice and guinea pigs.

Amino Acids↗
PubMed1993

Influence of Tołpa Peat Preparation on the IgE-induced anaphylactic reactions in mice.

The ability of Tołpa Peat Preparation (TPP) to affect anaphylactic sensitization and mast cell secretory function was tested in BALB/c mice treated with TPP orally for 12 days. TPP in the doses of 20 and 50 mg/kg/day reduced histamine release from mouse peritoneal mast cells challenged with anti-IgE or concanavalin A in vitro. The treatment of mice with TPP from day 1 to day 12 of immunization with Ovalbumin (OA) absorbed on aluminium hydroxide gel resulted in a decrease of antigen-induced histamine release from mast cells of these mice in vitro and in decreased IgE antibody level in their sera. TPP introduced into OA-immunized mice showing developed IgE antibody response was less effective in decreasing anaphylactic histamine release from mast cells of these mice. In all experiments low doses of TPP used for oral treatment were more effective than high doses in inhibiting anaphylactic events in the mice.

Amino Acids↗
PubMed1992

The immunomodulating preparation Ukrain does not induce anaphylactic sensitization in mice and guinea pigs.

The ability of Chelidonium majus L. alkaloids derivative Ukrain to induce an anaphylactic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera, and IgG1a, IgG1b as well as IgE antibody levels in guinea pig sera, were evaluated by passive cutaneous anaphylaxis (PCA) tests. Ukrain alone or adsorbed on aluminium hydroxide gel (alum) introduced into BALB/c mice in several subcutaneous injections was unable to stimulate measurable anti-Ukrain IgE antibody response. Moreover, Ukrain introduced together with ovalbumin (OA) into mice in the course of immunization with OA induced lower anti-OA antibody response as compared to the response induced by OA alone. Ukrain adsorbed on alum and injected subcutaneously into guinea pigs did not induce measurable IgG1a, IgG1b and IgE antibody response. The present results suggest that the immunomodulating preparation Ukrain could be therapeutically safe at least as far its inability to induce anaphylaxis is concerned.

Adjuvants, Immunologic↗
PubMed1990

Neurotransmitters in hepatic encephalopathy.

The pathogenesis of hepatic encephalopathy (HE), a neuropsychiatric disorder resulting from liver failure, is still a matter of debate. Recently attention has been focused on brain neurotransmitters. The accumulating evidence indicates that the imbalanced metabolism and turnover as well as the altered functions of neurotransmitters (dopamine, noradrenaline, serotonin, GABA) and false neurotransmitters (phenylethanolamine, octopamine, synephrine) may be of high importance in the pathomechanism of HE. Our data permit adding histamine (HA) to the multifactorial pathogenesis of HE. The enhanced rate of histidine influx into the brain following portocaval anastomosis and a dramatic increase in the HA content in the hypothalamus may suggest the involvement of HA in the symptomatology of hepatic encephalopathy.

Animals↗
PubMed1988

The response of histamine degrading enzymes to nematode infection.

The response of intestinal mucosal enzymes which metabolize histamine i.e. diamine oxidase (DAO), histamine N-methyltransferase (HMT), and monoamine oxidase (MAO), to infection with Nippostrongylus brasiliensis has been examined in mice and compared to the changes evoked by in vivo administration of compound 48/80. Infection with the parasite resulted in a significant decrease in the concentration of both amine oxidases, followed by recovery of MAO and an overshoot in DAO activity. HMT activity was enhanced at the beginning of infection, then decreased markedly by days 11 to 15, and sharply increased thereafter. Histamine levels were on average only 20% higher than the basal levels over the entire period studied, except on day 4 when they were slightly reduced. Histamine is alleged to be a potential inducing factor for degrading enzymes. Consistently, the histamine releaser 48/80 significantly elevated intestinal mucosal DAO and in some of the mice also increased HMT activity.

Amine Oxidase (Copper-Containing)↗
PubMed1988

Participation of aldehyde dehydrogenase in the oxidative deamination pathway of histamine and putrescine.

Some guinea pig tissue homogenates have shown the ability to catabolize, in vitro, imidazoloacetaldehyde (ImAAL) and gamma-aminobutyraldehyde (GABAL) via NAD-dependent aldehyde dehydrogenase (ALDH, EC 1.2.1.3). The liver, kidney, small intestine and gastric mucosa are the richest sources of ALDH activity towards ImAAL. The liver, kidney, small intestine and pancreas also show ALDH activity with GABAL as a substrate. All tissues tested have shown low Km and high Km ALDH activity with propionaldehyde as substrate. The guinea pig liver ALDH which is able to oxidize ImAAL and GABAL is located exclusively in the cytoplasm.

Aldehyde Dehydrogenase↗
PubMed1987

Causal relationship between a tumour growth and the changes in histamine metabolism in tissues of sarcoma-bearing rat.

The relationship between malignancy and histamine metabolism in the liver and the small intestine has been examined in sarcoma-bearing Wistar rats two weeks after subcutaneous implantation of a transplantable methylcholanthrene sarcoma Sa1828 and on the 3, 7 and 14th days after tumour extirpation. Two weeks after tumour implantation, the histamine level was increased by 100% and 50% in the liver and the small intestine, respectively. On the 3rd day after extirpation of the tumour the level of histamine had returned to the control values and remained unchanged during the next 10 days. Neither of the histamine catabolizing enzymes, diamine oxidase with a putrescine as a substrate or histamine methyltransferase were influenced by the existing tumour or by its extirpation except on the 14th day where a high increase in diamine oxidase activity was found. Some changes in the distribution of histamine metabolites suggest an involvement of an oxidative pathway of histamine catabolism as well as the aldehyde catabolizing enzymes in tumour development.

Amine Oxidase (Copper-Containing)↗
PubMed1986

Activation of hamster mast cells for IgE-mediated histamine release.

The conditions for active sensitization of hamster peritoneal and pleural mast cells and IgE-induced histamine release as well as cell desensitization were defined. Immunization of hamsters with ovalbumin (5 micrograms) in Al/OH/3 gel (5 mg) with several boosters resulted in sensitization of peritoneal and pleural mast cells; in the presence of extracellular Ca++, pH of medium 7.2 and at 37 degrees C these cells released up to 70% of histamine on the challenge with specific antigen. Partial release was observed when the cells were challenged with antigen in the absence of extracellular calcium. The rate of release is high during the first seconds of activation and is complete at 1 min. 30 min preincubation of peritoneal and pleural mast cells in calcium-free conditions (in the presence of 4 mM EDTA) resulted in complete desensitization of cells to subsequent action of antigen in optimal conditions. The present experiments demonstrate, that hamster peritoneal and pleural mast cells can be a useful model system for in vitro studies of the mechanisms of IgE-induced cell activation.

Animals↗
PubMed1986

Tritoqualine and some aspects of histamine metabolism.

The influence of in vivo tritoqualine (Inhibostamin) given to mice as a single dose (100 mg/kg) or subchronically (40 mg/kg daily for 5 days) has been examined on 1. histamine release from peritoneal mast cells, 2. histamine level in some tissues, and 3. intestinal diamine oxidase activity. The effects of tritoqualine and alpha-hydrazinohistidine injected into eggs on the 16th, 17th or 18th day of chick embryo age on hatching time, histamine level and diamine oxidase (DAO) activity in brood's tissues were also examined. After a single administration of tritoqualine there was a drop of histamine level in brain, a similar tendency being observed in kidney and lung, and an opposite effect in intestine. With the latter coincides the reduced intestinal DAO. Subchronical treatment changes pattern of anti-IgE induced histamine release from mast cells, slightly reduces histamine content in brain, kidney and lung, and transiently increases intestinal DAO. Neither tritoqualine nor alpha-hydrazinohistidine influenced time of hatching. Both drugs differ in respect to direction and intensity of changes they evoke in histamine content and DAO activity in examined organs.

Amine Oxidase (Copper-Containing)↗
PubMed1984

Histamine and its catabolism in tumour-bearing rat and mouse.

The hypothesis that failure of histamine (Hi)-mediated inter and intracellular cell-cell communication may be involved in the control of cellular growth has been tested in leukaemia-bearing mice and fibrosarcoma-bearing rats. In all examined tissues of mice bearing leukaemia L1210 cells and rats bearing methylcholanthrene fibrosarcoma histamine content was higher than in controls. Tissues of fibrosarcoma-bearing rats more intensively metabolized 14C-Hi and oxidative pathway was predominant. Histamine metabolic activity as well as histamine catabolic pathways do not differ in leukaemic mice as compared with the controls.

Animals↗
PubMed1983

Kinetics of specific IgE antibody and total IgE responses in mice: the effect of immunosuppressive treatment.

The IgE antibody and total IgE responses were studied in three mouse strains immunized with low doses of ovalbumin. BALB/c and C57BL/6J mice were considered as 'high' and 'intermediate' IgE antibody responders, respectively; during immunization the peritoneal mast cells of mice from these two strains became sensitized. In identical conditions of immunization, very low IgE antibody response and lack of mast cell sensitization was observed in CFW mice. After exposure of these CFW mice to low-dose X-irradiation (250 R) or a moderate dose of cyclophosphamide (100 mg/kg), a detectable IgE antibody response appeared and peritoneal mast cells became sensitized. In all three strains the evolution of total serum IgE levels during immunization did not correlate with IgE antibody response; an early transient elevation of total IgE level appeared soon after first immunization, at a time when IgE antibody responses were not detectable or were very low. After X-irradiation or cyclophosphamide pretreatment, this elevation of total IgE was potentiated. The possibility of differences in regulation of specific IgE antibody response and IgE of unknown specificity in the mice is discussed.

Animals↗
PubMed1982

Diamine oxidase activity and imidazoleacetic acid formation in the foetal and maternal guinea pig liver.

Diamine oxidase activity and imidazoleacetic acid formation in the foetal and maternal guinea pig liver during gestation were examined. DAO activity and IMAA formation in the foetal liver increased continuously, while maternal enzyme activity and ImAA formation in the second half of pregnancy simultaneously decreased. The roles of GABA and ImAA are discussed.

Amine Oxidase (Copper-Containing)↗
PubMed1982

Inhibition of plant and mammalian diamine oxidases by hydrazine and guanidine compounds.

1. Pig kidney and pea seedling diamine oxidases have similar sensitivity to methylhydrazine and phenylhydrazine as inhibitors. 2. Inhibition of pig kidney and pea seedling enzymes by hydrazine and guanidine compounds is time dependent. To reveal full inhibitory potency, methylhydrazine and aminoguanidine need longer preincubation with plant diamine oxidase as compared with mammalian diamine oxidase. 3. Impromidine, a known H2 histamine receptor agonist with guanidine and imidazole structures, and aminoguanidine have higher inhibitory activity towards pig kidney enzyme in comparison with the pea seedling one. 4. Impromidine inhibits pig kidney diamine oxidase in a noncompetitive manner. The Ki value is 6.6 muM. 5. The 24 hr dialysis of rat intestinal diamine oxidase preincubated with phenylhydrazine or impromidine only partially recovered the enzymic activities. 6. Impromidine inhibits mouse intestinal diamine oxidase in vivo.

Amine Oxidase (Copper-Containing)↗
PubMed1982

Gamma-aminobutyric acid (GABA) formation from putrescine in guinea-pig liver during ontogenesis.

1. The changes in hepatic diamine oxidase (DAO) activity of the foetal and maternal origin and their relations to GABA formation during pregnancy in guinea-pigs are described. 2. Foetal DAO activity continuously increased while the maternal enzyme from the 45th day of gestation onwards decreased. 3. Conversion of putrescine to GABA via oxidative deamination has been detected in the earliest studied day i.e. the 34th.

Amine Oxidase (Copper-Containing)↗
PubMed1982

The relationship between specific IgE antibody and non-specific IgE in anaphylactic sensitization of mice and rats.

The mice from inbred strains BALB/c, 129, C3H/A, C57BL/6J and outbred Wistar rats were tested for their capacity for reaginic sensitization on the response to antigenic stimuli. The total serum IgE level measured before immunization was highest in 129 mice and increased (2.5-3-fold) after immunization. BALB/c mice also showed an increase of the total serum IgE after immunization, whereas in two other strains such effect was not observed. All mouse strains tested produced IgE antibodies after immunization and the results suggest, that these IgE antibodies form only minor part of the total IgE content during immunization; in 129 and BALB/c mice beside IgE antibody response the production of non-specific IgE may be potentiated and these IgE may interfere in passive sensitization of mast cells with IgE antibodies. The examination of rats also showed, that mast cells with lower concentration of non-specific IgE are better acceptors for sensitization with IgE antibodies.

Age Factors↗
PubMed1981

In vivo formation of histamine phosphopyridoxal cyclic compounds.

A possibility of in vivo formation of cyclic compounds between histamine (Hi) given i.p. and endogenous pyridoxal (PL) or pyridoxal 5'-phosphate (PLP) has been studied. Cyclic compounds of Hi with PL or PLP were found in all tissues examined. Although an increase in Hi levels in tissues enhances the formation of cyclic compounds, no simple relationship between the rate of formation and Hi concentration has been observed. The reaction seems to be limited by endogenous PLP. The cyclic products Hi-PL and Hi-PLP were discovered in urine. It is suggested that the process of cyclic compound formation may reduce PLP resources, resulting in a modification of PLP-enzyme activities.

Animals↗