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C M Moore

Publications and source records attributed to C M Moore.

At least 145 records · Page 8Linked to original sources
PubMed1972

Factors affecting recognition and disjunction of chromosomes at distributive pairing in female Drosophila melanogaster. I. Total length vs. arm length.

The behavior of a compound metacentric fourth chromosome (see PDF) has been examined to determine whether arm length or total length is the basis for recognition in distributive pairing. Recognition was judged by the frequency with which the (see PDF) nondisjoined from a series of X duplications (Dp), ranging in size from </= 0.3 to > 4 times the size of a single fourth chromosome. Dp, (see PDF) nondisjunction was measured in the absence and in the presence of a competitor, a compound metacentric X. In both situations, total length and not arm length, was found to confer the characteristic recognition property to the (see PDF). A comparison of Dp, (see PDF) nondisjunction curves for both the noncompetitive and competitive situations with analogous Dp, 4 curves previously obtained, show the Dp, (see PDF) curves to be similar in shape to those obtained earlier but displaced one unit to the right, corresponding precisely to the difference in size between the (see PDF) and the 4. Rules governing chromosome recognition for acrocentrics were found completely applicable to metacentrics; disjunctive behavior of metacentrics differed from that of acrocentrics in that two arms conferred on a chromosome the capacity to act as the intermediate of a trivalent when size no longer warranted this attribute. This capacity, itself, is size-dependent.

Aneuploidy↗
PubMed1972

Factors affecting recognition and disjunction of chromosomes at distributive pairing in female Drosophila melanogaster. II. The effect of a second arm.

The behavior of heterozygously inverted X chromosomes that were members of the distributive pool at least 70% of the time was studied when the other pool members were either two free 4's or one compound 4. The X's were structurally modified by additions or deletions of heterochromatin, so that the two homologues differed in both size and configuration or in size alone. In the noncompetitive situation, with two free 4's, recognition between the X's remained high despite the modifications, and primary X nondisjunction was low. In the competitive situation, with the compound 4, distributive nondisjunction of the X's increased approximately two orders of magnitude, and trivalent formation was indicated. Disjunction from the trivalent varied with X size and configuration. When both X's were acrocentric, the smaller X directed the larger X and the very small (see PDF) to the same pole; when the larger X carried a second arm, it assumed the directing role; when the size ratio of the smaller, one-armed X to the larger, two-armed X became less than approximately 5/9, the smaller X again directed the other two.

Aneuploidy↗
PubMedDate not supplied

Rapid extraction of anti-inflammatory drugs in whole blood for HPLC analysis.

A rapid and efficient procedure is described for the extraction and analysis of anti-inflammatory drugs in whole blood. Red blood cells were fragmented by sonication and the blood sample extracted by passing through a bonded silica column (Bond-Elut). The adsorbed drugs were washed and eluted followed by analysis by HPLC. Recoveries were in excess of 80% at 5 micrograms/ml concentrations.

Acetaminophen↗
PubMedDate not supplied

Solid-phase cation exchange extraction of basic drugs from the urine of racing greyhounds.

The extraction of basic drugs from the urine of racing greyhounds has been carried out for many years using solvent extraction. This paper describes an extraction method that utilises a cation exchange column. Fourteen basic drugs were introduced onto the column in their ionised form, washed with organic and aqueous solvents and eluted with basic methanol. Three drugs, cyclizine, procaine and quinine were determined in authentic samples from racing greyhounds. A comparison of the solid-phase method with liquid-liquid extraction showed an increase in the efficiency of extraction and a reduction in both the time and cost incurred. The method requires a minimal volume of sample and solvent, little glassware and is easily automated.

Animals↗
PubMedDate not supplied

Rapid screening for 100 basic drugs and metabolites in urine using cation exchange solid-phase extraction and high-performance liquid chromatography with diode array detection.

A system involving the combination of cation exchange solid-phase extraction and gradient elution HPLC with diode array detection was developed for the isolation and identification of basic drugs in urine. At least 100 basic drugs commonly encountered in urinary drug screening and many popular illicit drugs could be identified. Detailed study of 30 of these compounds showed that in addition to the procedure's simplicity and convenience, recoveries were close to 100% in most cases. The method was compared with an existing liquid-liquid extraction/capillary gas chromatography procedure and was at least as effective for extracting and identifying basic drugs, and especially effective at extracting problematic compounds including morphine, benzoylecgonine, temazepam, and oxazepam. Additional advantages of solid-phase extraction technology include the elimination of time-consuming shaking and centrifugation steps, elimination of reusable glassware, and reduced sample-operator contact with potentially infectious samples.

Cations↗
PubMedDate not supplied

Solid-phase extraction of drugs from biological tissues--a review.

Solid-phase extraction procedures are being used increasingly in all types of drug testing laboratories, including those which carry out forensic toxicology. The advantages of such procedures include decreased operator time, reduced solvent volumes, and increased extraction efficiency. The application of existing techniques, however, has not yet been applied to tissues that are widely available postmortem. Tissue analysis can make the task of interpretation of drug levels much easier, particularly where unstable drugs are involved (e.g., cocaine). This review article explores the possibilities of applying existing extraction procedures to human tissue samples. We also consider the reasons why forensic laboratories have been slow to investigate such possibilities. We hope to stimulate interest in alternative tissue extraction procedures by reviewing reported procedures that apply to both human and animal tissue.

Animals↗
PubMedDate not supplied

Meconium analysis for cocaine: a validation study and comparison with paired urine analysis.

We established the validity of a drug-screening method to detect the presence of cocaine or benzoylecgonine or both in meconium and then undertook an analysis of results from urine and meconium specimens obtained concurrently from neonates within 3 days of birth. Meconium specimens from 82 consecutive newborns were analyzed using fluorescence polarization immunoassay (FPIA), Kinetic Interaction of Microparticles in Solution (KIMS), and gas chromatography-mass spectrometry (GC-MS). Each meconium specimen was analyzed by all three methods. Fifty-four paired urine and meconium specimens were obtained over a 13-month period from a neonatal intensive care unit. Urine drug testing was performed by immunoassay (enzyme multiplied immunoassay [EMIT] technique), whereas meconium specimens utilized FPIA with GC-MS confirmation on all but one specimen (due to insufficient quantity). Ten true positives were found by GC-MS, 10 positives were found by FPIA, and 70 positives were found by KIMS. Of the 54 paired samples, 39 samples tested negative for cocaine in both urine and meconium; four specimens were positive by both routes; 10 specimens were negative in urine but positive in the meconium; and one specimen tested positive in urine but negative in the meconium. Thus, 9.3% of the urine specimens tested positive, and 25.9% of meconium samples tested positive (p = .011; McNemar's Test). We conclude that screening meconium specimens by FPIA followed by GC-MS confirmation of screened positives yields highly accurate determinations of the presence of cocaine or benzoylecgonine or both in meconium and that testing of meconium for cocaine and its metabolites is more sensitive than testing of urine.

Cocaine↗