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Biomedical subjects

C M CARPENTER

Publications and source records attributed to C M CARPENTER.

16 recordsLinked to original sources
PubMed1958

Tissue culture studies on bacterial allergy in experimental brucellosis. I. The effect of Brucella suis whole antigen on cultures of spleen from normal and brucella-infected guinea pigs.

Tissue culture methods have been used to investigate infectious allergy in experimental brucellosis. A study was made of the effect of whole cell Brucella antigen on cultures of spleen from normal and Brucella-infected guinea pigs. The degree of toxicity was based upon the inhibition of migration of wandering cells and upon the morphologic appearance of stained sections of tissue cultures at different periods of incubation. A suspension of heat-killed Br. suis was more toxic for splenic cells from guinea pigs infected with Br. suis than for normal splenic cells. Macrophages were more sensitive than leucocytes to the toxic action of the antigen. The degenerative changes observed in Brucella-sensitive cells exposed to the antigen were similar to the degeneration previously observed in cultures of tuberculin-sensitive cells in the presence of tuberculin. The specific toxicity of the whole Brucella antigen, however, was more marked than that of tuberculin. Preliminary experiments indicate that serum and plasma containing specific antibodies obtained from Brucella-infected guinea pigs reduce the toxic effect of the antigen in cultures of both normal and Brucella-sensitive cells. The protective action of the homologous antiserum was greater for Brucella-sensitive cells than for normal cells.

Animals↗
PubMed1958

Reiter protein complement fixation test; a preliminary comparison of the TPI test with the complement fixation test employing a soluble protein antigen derived from the Reiter strain.

A comparative study of the specificity of the Reiter protein complement fixation (RPCF) test and the Treponema pallidum immobilization (TPI) test on 180 sera showed that the results in 178 instances or 98.9 per cent were in agreement. The sensitivity of the RPCF test, when compared to the TPI test, on 189 sera from patients known to have syphilis was in agreement in 182 or 96.3 per cent, assuming a correlation exists between positive TPI and both the "reactive" or "weakly reactive" RPCF test results. As to reproducibility of results, the RPCF test results agreed in 84 of the 87 sera tested (95.4 per cent). The sera were tested at least three times on different days. Anticomplementary reactions were observed in three of 91 normal sera.

Complement Fixation Tests↗
PubMed1956

Serologic diagnosis of syphilis; the use of Treponema pallidum immobilization and immune adherence tests.

TPI tests were carried out on 4,060 sera. Among 3,934 patients with reactive STS and no history of syphilis, 2,148 or 54.6 per cent had negative reaction to TPI tests, 1,695 or 43.1 per cent had positive reaction and 91 or 2.3 per cent had doubtful reaction. Two hundred and ninety-two or 73.0 per cent of 400 pregnant women with reaction to STS in the absence of a history of syphilis showed negative results by TPI test, 103 or 25.8 per cent had positive results and five or 1.2 per cent had doubtful reaction.Ninety-five or 75.4 per cent of 126 patients with a history of treated syphilis had positive reaction to TPI tests, 20 or 20.2 per cent had negative reaction and nine or 9.1 per cent had doubtful reaction.TPI and TPIA tests were done on 143 sera carefully selected for the study. Among 102 sera subjected to the TPI test, 46 or 100 per cent of these positive were also positive by TPIA tests, while 52 or 94.5 per cent of 55 TPI-negative sera were also nonreactive by TPIA test. One serum gave doubtful TPI test reaction and positive TPIA test reaction.

Female↗
PubMed1955

Ratbite fevers.

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Humans↗
PubMed1952

Coxsackie viruses; a review of pathologic, epidemiologic, diagnostic and etiologic observations.

Coxsackie disease comprises three clinical entities-herpangina, so-called non-paralytic poliomyelitis, and epidemic pleurodynia. Several strains of antigenically-related viruses, Groups A and B, designated as Coxsackie virus have been isolated from stool specimens and from material from the throat of many patients with the diseases mentioned. Inasmuch as the virus has also been recovered from normal persons, there is as yet uncertainty as to causal relationship between the presence of the virus and the disease. Reports of the isolation of Coxsackie virus and poliomyelitis virus from the same patient make difficult the interpretation of the findings. The diagnosis of Coxsackie disease entails animal inoculation and serologic procedures. Emphasis is placed on the necessity of obtaining stool specimens, throat washings, and "paired" blood specimens from patients suspected of the disease.

Animals↗