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Biomedical subjects

C Lundqvist

Publications and source records attributed to C Lundqvist.

At least 55 records · Page 3Linked to original sources
PubMed1990

Spinal arteriovenous malformations: neurological aspects and results of embolization.

Twenty consecutive patients with spinal arteriovenous malformations (AVMs) were found to have either a progressive, remitting or remitting-progressive course of disease. A characteristic progressive neurological decline was observed in 11 patients with dural fistulas and a similar decline was seen in 5 of the 9 patients with intradural AVMs. In addition to paraparesis and mixed sensory disorder, which ascended to a level of about T10, areas with spared sensation were occasionally found below this level. All cases with this progressive longitudinal myelopathy showed radiological evidence of a dilated medullary venous plexus. All patients were treated by embolization with only a low frequency of complications. Follow-up data verified a significant improvement of motor and sensory function, mainly during the first year after embolization. Cases with progressive neurological decline (dural and intradural AVMs) stopped deteriorating or improved and cases with a history of acute incidents (intradural AVMs) probably run less risk of acute deterioration.

Adult↗
PubMed1989

Liquid chromatographic separation of cerebrospinal fluid amino acids after precolumn fluorescence derivatization.

Sampling and HPLC analysis procedures for CSF amino acid determination were evaluated. It is recommended to analyse native CSF. The sample should be taken from the first 12 ml obtained by lumbar puncture. Amino acid concentrations are stable for short periods over a wide range of temperature however, storage at -70 degrees to -80 degrees C is recommended. With the precolumn derivatization and a 30 cm reverse-phase column, the separation and reproducibility are reliable for analyses in the daily hospital routine.

Adult↗
PubMed1988

Monoclonal antibodies to the 27-34K insulin-like growth factor binding protein.

Monoclonal antibodies were prepared against the 27-34K insulin-like growth factor (IGF)-binding protein purified from human placenta/decidua and designated placental protein 12 (PP12). Four different antibodies were characterized. Each recognized the major band at 32K on immunoblots of the purified PP12 preparation and amniotic fluid. In liquid phase RIA, IGF-I did not affect the binding of [125I] PP12 to one antibody (Mab 6303), it slightly increased the binding to two antibodies (Mab 6301 and 6304), and it slightly decreased the binding to one antibody (Mab 6302). All antibodies immunoprecipitated the cross-linked PP12-[125I] IGF-I complex, but Mab 6302 considerably less effectively than the others. Preincubation of PP12 with Mab 6302 completely inhibited the binding of [125I] IGF-I to PP12, whereas preincubation with Mab 6303 had no effect, and Mab 6301 as well as Mab 6304 increased it. These results suggest that Mab 6302 binds to an epitope at or near to the IGF-binding site, whereas the other antibodies react at other sites of the PP12 molecule. Conformational changes in PP12 probably account for the IGF-I-induced increase in the binding of Mabs 6301 and 6304 to [125I] PP12, and vice versa, for Mabs 6301- and 6304-induced increase in the binding of [125I] IGF-I to PP12.

Antibodies, Monoclonal↗
PubMed1987

Influence of halo vest treatment on vital capacity.

Respiratory function (vital capacity) was studied in 20 consecutive patients with unstable cervical spine injuries treated with a halo vest. Eight patients were neurologically intact. Twelve patients had incomplete spinal cord injuries that were classified on a neurologic function scale (Sunny-brook) immediately and 3 months after injury. Spirometric tests were done within 1 week of halo vest fixation, after 3 months of treatment, and 1 week after dismounting of the halo vest. The results showed that initial vital capacity was smaller than predicted normal in all patients and 30% less in neurologically impaired patients. Both groups improved during the treatment and somewhat more after removal of the halo vest. In neurologically intact patients, the halo vest caused a respiratory restriction of 10%, which was fully regained after removal of the halo vest. The difference between the groups remained throughout the study. There was no evidence that the halo vest itself affects the vital capacity more in patients with incomplete cord lesions than in neurologically intact patients. All of the cervical spine injuries healed uneventfully.

Adolescent↗
PubMed1983

Assignment of gene(s) coding for antigen defined by monoclonal antibody 2B2.

A mouse monoclonal antibody (2B2) recognizes an antigen which is present on most human peripheral blood leukocytes but is absent from most proliferating cells. The antibody precipitated two surface-labeled membrane glycopolypeptides with molecular weights of 86,000 and 145,000, and it was strongly mitogenic to normal human lymphocytes. Somatic cell hybrids have been used for assigning the genes coding for these membrane glycoproteins to human chromosome 21. The assignment was based on correlation of antigen expression on mouse-human T-lymphocyte hybrids with the presence of human chromosomes in the same hybrid clones.

Animals↗
PubMed1982

Specificity of monoclonal antibodies to an EBV transformed B-cell line.

Monoclonal antibodies were produced against an Epstein Barr virus (EBV) transformed human B-cell line with the following HLA specificities: HLA A2, B27, Cw2, Dr3,2. Antibodies from three clones, Mab B1, Mab B2 and B3 reacted with human Ia-like molecules on peripheral blood B cells, some monocytes, CLL cells, lymphoblastoid B-cell lines and some mixed leukocyte culture (MLC)-activated T cells, but were unreactive with leukoagglutinin (LA) and Concanavalin A (Con A)-activated T blasts and T-cell lines. Antibodies obtained from three other clones (Mab 4, 5 and 6) reacted with a mw 80,000 protein present on peripheral lymphocytes and on most of the lymphoblastoid T-and B-cell lines tested.

Antibodies, Monoclonal↗
PubMed1981

The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas.

A monoclonal mouse antibody to human chorionic gonadotropin (hCG) was used in a modified unlabeled antibody enzyme-bridge staining method to demonstrate the localization of hCG in normal human placenta, pituitary gland, and six pituitary chromophobe adenomas. Mouse ascitic fluid containing monoclonal antibody could be diluted up to 1:500,000 for detection of hCG in the syncytiotrophoblast, whereas no staining was observed in the pituitary or adenomas even with high antibody concentrations (dilutions from 1:500 upward). Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies are suitable for immunohistochemical localization of antigens in tissues.

Adenoma↗
PubMed1979

Heteromorphic X chromosomes in 46,XX males?

This paper reports an attempt to determine whether the short arm of one of the X chromosomes in XX males is longer than normal. In a blind study comparing coded photomicrographs of 15 G-banded mitoses from each of five XX males and five control females, the results were ambiguous and somewhat contradictory, but gave the impression of, or were compatible with, an XXp+ phenomenon in at least two of the five XX males. Measurements of the X chromosomes from the above cells and, in addition, from 15 mitoses from each of six XXY males, failed to disclose any XXp+ phenomenon. Statistical analysis indicated that in the five XX males there was no difference in the lengths of the two Xp arms. The reasons for the apparent discrepancy between the results of ocular inspection and measurement are discussed. The putative heteromorphism might be an alteration in shape, staining intensity, or position of bands, neither of which necessarily leads to an increase in length. We conclude that our results do not indicate any XXp+ phenomenon in the five XX males tested. However, the presence or absence of XXp+ is not in itself evidence for or against interchange betweenthe X and Y in the paternal meiosis. Our results emphasize that the etiology of XX males is likely to be heterogeneous.

Female↗
PubMed1978

Stimulation of human fetal lymphocytes by lipopolysaccharide B in culture. Studies on cells circulating in maternal blood.

Lipopolysaccharide B (LPS) was used in attempts to stimulate fetal cells circulating in the maternal blood during pregnancy. Crystalline leukoagglutinin (LA), the mitogenic properties of which are identical with those of phytohemagglutinin, was used as a reference mitogen. When artificial mixtures of varying proportions of lymphocytes from mothers (XX) and their newborn male infants (XY) were co-cultured in the presence of these mitogens. LPS brought about a definite enrichment of XY mitoses, indicating that even under these conditions of co-culture, LPS preferentially, though not exclusively, stimulates the infant cells. When cells from the blood of 13 women pregnant in the second trimester with a male fetus were cultured with LPS as a mitogen, all mitoses were found to be XX. Since interphase Y chromatin occurred in uncultured lymphocytes from these women (cells containing Y chromatin were found in 5 out of 6 women tested), we conclude that LPS is unable to stimulate, or at least induce mitoses, in fetal cells circulating in the blood of pregnant women. The nature of these cells is discussed.

Adult↗
PubMed1978

Cell surface markers in cord blood leucocytes after stimulation with lipopolysaccharide B.

T- and B-lymphocyte markers were studied in cord blood cells cultured with lipopolysaccharide B (LPS). Cells cultured with leucoagglutinin (LA) and pokeweed mitogen (PWM) were used as controls. LPS-induced lymphoblasts were negative for surface Ig, positive for intracellular Ig and did not form rosettes with sheep red blood cells (SRBC). LA-activated cells formed rosettes with SRBC, while PWM cultures showed a varying proportion of surface Ig-positive or SRBC rosetting cells, dependent on the time of culture. About 50% of both LA- and LPS-activated lymphoblasts formed EA rosettes (specific for Fc receptors) and EAC rosettes (specific for complement receptors). The response of foetal cells to LPS was reduced when lymphocytes obtained from Isopaque-Ficoll gradients were passed through nylon wool columns, whereas this procedure led to an increased response to LA. Thus LPS-activated foetal leucocytes are B lymphocytes expressing intracellular but not surface Ig.

B-Lymphocytes↗
PubMed1978

Activation of lymphocytes in CLL by protein A from Staphylococcus aureus.

The mitogenic activity of protein A (PA), leucoagglutinin (LA) and pokeweed mitogen (PWM) was tested in six cases of chronic lymphatic leukaemia (CLL), and the response to PA and LA in six healthy controls. The effect of foetal calf serum (FCS) and pooled human AB serum in the cultures was tested in two patients. The response to LA in the patients was about one tenth of that in the controls. The response to PA was strong both in patients and in healthy controls. However, in lymphocytes from CLL patients this response was serum-dependent, being very strong with FCS in the culture medium, but absent when the medium contained human AB serum. Lymphocytes from normal controls responded to PA in the presence of AB serum.

Agglutinins↗
PubMed1977

Stimulation of human fetal lymphocytes by lipopolysaccharide B in culture.

Cultures of Isopaque-Ficoll-isolated lymphocytes from three human sources were compared with respect to the effect of mitogens. The cell sources were maternal blood immediately after delivery, cord blood, and blood obtained by heart puncture of 10-20-week aborted fetuses. Lipopolysaccharide B (LPS) induced incorporation of tritiated thymidine, blastic transformation, and mitotic activity in cord and fetal, but not maternal, cells. The stimulation reached a maximum on days 4-8 of culture. It was stronger than the spontaneous transformation often displayed by fetal cells. If fetal cells spontaneously occurring in the blood of pregnant women were to react in a similar way, it should be possible to selectively stimulate the fetal cells with LPS. Such transformed fetal cells could then be isolated from cultures of maternal blood samples and used for antenatal diagnosis of fetal disease.

Cells, Cultured↗